Journal of ethnopharmacology · 2026
The potential of Kadsura longipedunculata against herb and chemical induced liver injury.
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Qin Ma-Long, Qian Yan-Ling, Wen Xi, Zhao Yun-Li, Luo Xiao-Dong
Аннотация
ETHNOPHARMACOLOGICAL RELEVANCE: Kadsura longipedunculata is a Yi ethnic herb for hepatic disorders treatment in China. However, its effects on herb- and chemical-induced liver injury remain unknown.
AIM OF THE STUDY: To validate traditional ethnomedicinal applications of K. longipedunculata and characterize its bioactive constituents.
MATERIALS AND METHODS: The hepatoprotective effect was evaluated using a triptolide-induced mice liver injury model. Bio-guided isolates were screened in an acetaminophen (APAP)-induced HepG2 cell model. Underlying signaling pathways were investigated using transcriptomics, network pharmacology, molecular docking, molecular dynamics simulations, and subsequent Western blotting validation.
RESULTS: Abnormal biochemical indices, antioxidant defenses, and liver pathological necrosis in triptolide-induced mice were all adjusted by treatment with K. longipedunculata extract, exhibiting effects superior to those of the positive control, silymarin. The PI3K/AKT/NF-κB signaling pathway was identified as a core therapeutic pathway through transcriptomic profiling, supported by the upregulation of phosphorylated AKT and MTOR concurrent with the downregulation of NF-κB and TNF-α. Four compounds were isolated, among which catechin-5-O-β-D-glucopyranoside (Catechin GP) showed potent anti-apoptotic and antioxidative bioactivities in APAP-induced hepatocytes through the same pathway. The formation of a stable Catechin GP-AKT1 complex was further supported by computational analyses of binding stability and safety.
CONCLUSION: The protective effect of K. longipedunculata and its bioactive compound, Catechin GP, against liver injury triggered by both herbal (triptolide) and chemical (acetaminophen) agents is regulated via the PI3K/AKT/NF-κB pathway.
AIM OF THE STUDY: To validate traditional ethnomedicinal applications of K. longipedunculata and characterize its bioactive constituents.
MATERIALS AND METHODS: The hepatoprotective effect was evaluated using a triptolide-induced mice liver injury model. Bio-guided isolates were screened in an acetaminophen (APAP)-induced HepG2 cell model. Underlying signaling pathways were investigated using transcriptomics, network pharmacology, molecular docking, molecular dynamics simulations, and subsequent Western blotting validation.
RESULTS: Abnormal biochemical indices, antioxidant defenses, and liver pathological necrosis in triptolide-induced mice were all adjusted by treatment with K. longipedunculata extract, exhibiting effects superior to those of the positive control, silymarin. The PI3K/AKT/NF-κB signaling pathway was identified as a core therapeutic pathway through transcriptomic profiling, supported by the upregulation of phosphorylated AKT and MTOR concurrent with the downregulation of NF-κB and TNF-α. Four compounds were isolated, among which catechin-5-O-β-D-glucopyranoside (Catechin GP) showed potent anti-apoptotic and antioxidative bioactivities in APAP-induced hepatocytes through the same pathway. The formation of a stable Catechin GP-AKT1 complex was further supported by computational analyses of binding stability and safety.
CONCLUSION: The protective effect of K. longipedunculata and its bioactive compound, Catechin GP, against liver injury triggered by both herbal (triptolide) and chemical (acetaminophen) agents is regulated via the PI3K/AKT/NF-κB pathway.