Wei sheng yan jiu = Journal of hygiene research · 2026
[Establishment of a rat model of hepatointestinal injury induced by high-fat diet combined with dextran sulfate sodium].
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Feng Jing, Han Chao, Zhao Jinpeng, Chen Chen, Li Rongzi, Sun Chaoqun, Wang Liyuan, Zhuo Qin
Аннотация
OBJECTIVE: To establish and validate a comorbid model of non-alcoholic fatty liver disease(NAFLD) and chronic colitis induced by a combination of a high-fat diet(HFD) and dextran sulfate sodium(DSS) in drinking water.
METHODS: Thirty-six male SD rats were randomly assigned to six groups: control(CON), high-fat diet(HFD, 45% energy from fat), 1%DSS(DSS1.0), 1.5%DSS(DSS1.5), HFD combined with 1%DSS(HFD+DSS1.0), and HFD combined with 1.5%DSS(HFD+DSS1.5). After 8 weeks of feeding, serum, liver, and colon tissues were collected. Lipid profiles, liver function, hepatic oxidative stress levels, colonic mucosal integrity, and inflammatory status were assessed in each group.
RESULTS: Regarding liver injury, liver wet weight and liver index were significantly higher in the three HFD-fed groups(HFD, HFD+DSS1.0 and HFD+DSS1.5 groups) compared to the CON group. Histopathological scores were elevated, and serum levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST), total cholesterol(TC), triglycerides(TG), and low-density lipoprotein cholesterol(LDL-C) were increased, while high-density lipoprotein cholesterol(HDL-C) levels were decreased. Moreover, hepatic TC, TG, malondialdehyde(MDA), and protein carbonyl contents were also elevated, and superoxide dismutase(SOD) content was reduced. No significant differences were observed in the aforementioned indices among the three high-fat diet groups, nor were there significant differences between the two DSS-only intervention groups and the CON group. In terms of intestinal injury, compared to the CON group, the HFD+DSS1.5 group exhibited decreased colonic zonula occludens-1(ZO-1) content, alongside increased levels of tumor necrosis factor-alpha(TNF-α) and interleukin-6(IL-6), and higher histopathological scores. No statistically significant differences in the relevant indicators were observed among the HFD, DSS1.0, DSS1.5, and HFD+DSS1.0 groups compared to the control group. Furthermore, the TNF-α level in the HFD+DSS1.5 group was higher than that in the DSS1.5 group with the same DSS concentration. Compared to the HFD+DSS1.0 group, the HFD+DSS1.5 group demonstrated higher levels of TNF-α and IL-6 and a higher intestinal histology score.
CONCLUSION: An 8-week regimen of a high-fat diet(45% energy from fat) combined with 1.5% DSS in drinking water successfully induced a comorbid liver-intestine disease model in rats.
METHODS: Thirty-six male SD rats were randomly assigned to six groups: control(CON), high-fat diet(HFD, 45% energy from fat), 1%DSS(DSS1.0), 1.5%DSS(DSS1.5), HFD combined with 1%DSS(HFD+DSS1.0), and HFD combined with 1.5%DSS(HFD+DSS1.5). After 8 weeks of feeding, serum, liver, and colon tissues were collected. Lipid profiles, liver function, hepatic oxidative stress levels, colonic mucosal integrity, and inflammatory status were assessed in each group.
RESULTS: Regarding liver injury, liver wet weight and liver index were significantly higher in the three HFD-fed groups(HFD, HFD+DSS1.0 and HFD+DSS1.5 groups) compared to the CON group. Histopathological scores were elevated, and serum levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST), total cholesterol(TC), triglycerides(TG), and low-density lipoprotein cholesterol(LDL-C) were increased, while high-density lipoprotein cholesterol(HDL-C) levels were decreased. Moreover, hepatic TC, TG, malondialdehyde(MDA), and protein carbonyl contents were also elevated, and superoxide dismutase(SOD) content was reduced. No significant differences were observed in the aforementioned indices among the three high-fat diet groups, nor were there significant differences between the two DSS-only intervention groups and the CON group. In terms of intestinal injury, compared to the CON group, the HFD+DSS1.5 group exhibited decreased colonic zonula occludens-1(ZO-1) content, alongside increased levels of tumor necrosis factor-alpha(TNF-α) and interleukin-6(IL-6), and higher histopathological scores. No statistically significant differences in the relevant indicators were observed among the HFD, DSS1.0, DSS1.5, and HFD+DSS1.0 groups compared to the control group. Furthermore, the TNF-α level in the HFD+DSS1.5 group was higher than that in the DSS1.5 group with the same DSS concentration. Compared to the HFD+DSS1.0 group, the HFD+DSS1.5 group demonstrated higher levels of TNF-α and IL-6 and a higher intestinal histology score.
CONCLUSION: An 8-week regimen of a high-fat diet(45% energy from fat) combined with 1.5% DSS in drinking water successfully induced a comorbid liver-intestine disease model in rats.