The Journal of allergy and clinical immunology · 2026
CD25 expression marks an activated mast cell population in human nasal polyposis.
СтатьяПоддержка NIH: внешняя программаПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Santos Rizza U, Suber Jada, Derakhshan Tahereh, Gullapalli Sri Vidya Niharika, Matsuda Kenshiro, Zawacki Mabel C, Lai Juying, Bergmark Regan W, Lee Stella E, Maxfield Alice Z, Roditi Rachel E, Baloh Carolyn H, Laidlaw Tanya M, Boyce Joshua A, Dwyer Daniel F
Аннотация
BACKGROUND: Chronic rhinosinusitis with nasal polyps (CRSwNP) is a TH2 disease characterized by heterogeneous mast cell hyperplasia. We previously used transcriptomics to identify discrete gene expression linked with intraepithelial mast cells expressing tryptase alone (MCTs), subepithelial mast cells coexpressing tryptase and chymase (MCTCs), and immature mast cells in CRSwNP. Although MCTCs were the only subset consistently observed in both CRSwNP and chronic rhinosinusitis without nasal polyps (CRSsNP), the degree to which they differ across disease states remains unexplored.
OBJECTIVE: We sought to use transcriptomics and flow cytometry to compare MCTCs in CRSwNP with those in CRSsNP.
METHODS: MCTCs were flow-sorted from CRSwNP and CRSsNP samples for RNA-sequencing analysis to identify differentially expressed genes and pathways across disease states. In silico observations were validated using flow cytometry on cryopreserved tissue samples from CRSwNP and CRSsNP and RNA sequencing of stimulated ex vivo-differentiated MCs.
RESULTS: We found a significant increase in MCTC concentrations in CRSwNP donors, which were enriched for activation-associated pathways linked with differential expression of transcripts encoding proinflammatory mediators and cell receptors, including IL2RA. Validation studies highlighted significant cell surface expression of CD25 on MCTCs in CRSwNP. Ex vivo-stimulated primary MCs upregulated IL2RA in response to IL-33 stimulus.
CONCLUSIONS: Our data show that MCTCs in CRSwNP exhibit an activated transcriptional phenotype with expression of transcripts encoding proinflammatory mediators and IL2RA. Moreover, the CD25 upregulation on MCTCs in CRSwNP suggests its potential to serve as an in vivo marker of activated MCTCs during allergic disease.
OBJECTIVE: We sought to use transcriptomics and flow cytometry to compare MCTCs in CRSwNP with those in CRSsNP.
METHODS: MCTCs were flow-sorted from CRSwNP and CRSsNP samples for RNA-sequencing analysis to identify differentially expressed genes and pathways across disease states. In silico observations were validated using flow cytometry on cryopreserved tissue samples from CRSwNP and CRSsNP and RNA sequencing of stimulated ex vivo-differentiated MCs.
RESULTS: We found a significant increase in MCTC concentrations in CRSwNP donors, which were enriched for activation-associated pathways linked with differential expression of transcripts encoding proinflammatory mediators and cell receptors, including IL2RA. Validation studies highlighted significant cell surface expression of CD25 on MCTCs in CRSwNP. Ex vivo-stimulated primary MCs upregulated IL2RA in response to IL-33 stimulus.
CONCLUSIONS: Our data show that MCTCs in CRSwNP exhibit an activated transcriptional phenotype with expression of transcripts encoding proinflammatory mediators and IL2RA. Moreover, the CD25 upregulation on MCTCs in CRSwNP suggests its potential to serve as an in vivo marker of activated MCTCs during allergic disease.