Vaccines · 2025
Visual Detection of Malaria Parasite-Parasitized Erythroblasts in Peripheral Blood via Immunization-Based Model.
СтатьяНазвание и аннотация: Перевод в очереди
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Ito Kumpei, Tateishi Yuki S, Imai Takashi, Miyazaki Shinya, Miyazaki Yukiko, Kagaya Wataru, Nakashima Mai, Sase Miho, Yoshioka-Takeda Misato, Shimokawa Chikako, Hayashi Kyoko, Itokawa Kentaro, Komagata Osamu, Ngo-Thanh Ha, Shimo Aoi, Araki Tamasa, Annoura Takeshi, Murakami Takashi, Hisaeda Hajime
Аннотация
BACKGROUND: Erythroblasts have recently been identified as host cells for malarial parasites, revealing a previously underappreciated host-parasite interaction. However, their extremely low abundance in peripheral blood has hindered progress, especially in elucidating the biological significance of parasitized erythroblasts (pEBs) in vivo.
METHODS: Here, we visualized pEBs in a murine model and established a method to increase their number in peripheral blood by immunizing mice with live Plasmodium yoelii 17XNL, followed by challenge with P. berghei ANKA.
RESULTS: Immunized mice were protected from cerebral malaria and survived longer, during which pEBs appeared in circulation and were detected using Giemsa-stained smears. All blood-stage parasite forms were identified within pEBs, including enucleating erythroblasts.
CONCLUSIONS: This model enables in vivo/ex vivo analysis of pEB biology without bone marrow/spleen isolation, thus lowering technical/ethical barriers for the field.
METHODS: Here, we visualized pEBs in a murine model and established a method to increase their number in peripheral blood by immunizing mice with live Plasmodium yoelii 17XNL, followed by challenge with P. berghei ANKA.
RESULTS: Immunized mice were protected from cerebral malaria and survived longer, during which pEBs appeared in circulation and were detected using Giemsa-stained smears. All blood-stage parasite forms were identified within pEBs, including enucleating erythroblasts.
CONCLUSIONS: This model enables in vivo/ex vivo analysis of pEB biology without bone marrow/spleen isolation, thus lowering technical/ethical barriers for the field.