Frontiers in bioengineering and biotechnology · 2026
Establishment of an immortalized porcine erythroid progenitor cell line using species-adapted papillomavirus oncogenes.
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Tran Tra My, Lee Hyeon Ho, Hwang Tae Hyeon, Kim Min Hyuk, Dang Nhat Minh, Lee Min Guk, Shim Joohyun, Hwang Jeong Ho, Kim Jae Young
Аннотация
INTRODUCTION: Critically low blood donation rates and recurrent shortages in the blood supply have become pressing challenges in transfusion medicine. Xenotransfusion using porcine red blood cells (pRBCs) represents a promising alternative; however, risks of immune rejection and infection necessitate the development of genetically engineered porcine models - a process that is time-consuming, costly, and technically complex. In this study, we established an immortalized porcine erythroid progenitor cell line as a platform for genetic manipulation and the evaluation of xenotransfusion potential.
METHODS: Porcine hematopoietic progenitor cells (CD117+) were isolated from bone marrow mononuclear cells and immortalized via lentiviral transduction with E6/E7 oncogenes from Human papillomavirus type 16 (HPV16) and Sus scrofa papillomavirus (SPV2).
RESULTS: The resulting cells were optimized for long-term culture and exhibited stable proliferation. The immortalized porcine erythroid progenitor cells retained the capacity to differentiate into red blood cells.
DISCUSSION: Our findings demonstrate that E6/E7-mediated immortalization facilitates the generation of porcine erythroid progenitor cells capable of differentiating into red blood cells, offering a valuable tool for genetic engineering and a promising avenue toward safe, sustainable heterologous blood transfusion strategies.
METHODS: Porcine hematopoietic progenitor cells (CD117+) were isolated from bone marrow mononuclear cells and immortalized via lentiviral transduction with E6/E7 oncogenes from Human papillomavirus type 16 (HPV16) and Sus scrofa papillomavirus (SPV2).
RESULTS: The resulting cells were optimized for long-term culture and exhibited stable proliferation. The immortalized porcine erythroid progenitor cells retained the capacity to differentiate into red blood cells.
DISCUSSION: Our findings demonstrate that E6/E7-mediated immortalization facilitates the generation of porcine erythroid progenitor cells capable of differentiating into red blood cells, offering a valuable tool for genetic engineering and a promising avenue toward safe, sustainable heterologous blood transfusion strategies.