Interactions between eosinophils and IL-5Rα-positive mast cells in nonadvanced systemic mastocytosis.
Обработка статьи · этапы, ошибки и управление
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Lefèvre Guillaume, Gibier Jean-Baptiste, Bongiovanni Antonino, Lhermitte Ludovic, Rossignol Julien, Anglo Emilie, Dendooven Arnaud, Dubois Romain, Terriou Louis, Launay David, Barete Stéphane, Esnault Stéphane, Frenzel Laurent, Gourguechon Clément, Ballul Thomas, Dezoteux Frédéric, Staumont-Salle Delphine, Copin Marie-Christine, Rignault-Bricard Rachel, Maciel Thiago Trovati, Damaj Gandhi, Tardivel Meryem, Crinquette-Verhasselt Marie, Dubreuil Patrice, Maouche-Chrétien Leila, Bruneau Julie, Lortholary Olivier, Duployez Nicolas, Behal Hélène, Molina Thierry Jo, Hermine Olivier
Аннотация
OBJECTIVE: We described blood and BM eosinophil characteristics in SM.
METHODS: A large collection of BM biopsy samples was analyzed using immunohistochemical staining and whole-slide imaging. Eosinophil and extracellular granules were detected by eosinophil peroxidase (EPX) staining and MCs by KIT staining. Complementary analyses were conducted using flow cytometry and immunofluorescence.
RESULTS: Eosinophil infiltrates and large areas of eosinophil degranulation were observed within or around BM MC infiltrates in SM. EPX staining surface, highlighting intact eosinophils and eosinophil degranulation, was higher in nonadvanced SM (n = 37 BM biopsy samples) compared with both controls (n = 8, P = .0003) and advanced SM (n = 24, P = .014). In nonadvanced SM, positive correlations were observed between serum tryptase levels and percentages of eosinophil counts in BM aspirations (Spearman r coefficient r = 0.38, P = .038), eosinophils count in BM biopsy samples (r = 0.45, P = .007), EPX staining (r = 0.37, P = .035), and eosinophil degranulation (r = 0.39, P = .023). Eosinophil counts in BM biopsy samples also correlated with MC counts (r = 0.47, P = .006) and KIT staining surface (r = 0.49, P = .003). BM MCs expressed IL-5 receptor and other usual eosinophil cytokine/chemokine receptors, and blood eosinophils displayed several increased surface markers compared with controls, suggesting an activated state.
CONCLUSION: Our data suggest possible cross talk between MCs and eosinophils, supporting MC tryptase release and MC activation-related symptoms. This suggests a rationale for targeting eosinophils in nonadvanced SM not fully controlled by other therapies.