Isolation and Culture of Leptospira from Clinical and Environmental Samples.

Leptospires, the etiological agents of leptospirosis, are fastidious, slow-growing organisms. Here, we describe the isolation and routine maintenance of leptospires from clinical (blood, urine, or tissue) and environmental (water or soil) samples. Using combinations of filtration, agar plating, and selective agents, leptospires can be isolated in pure cultures even from complex contaminated sources in standard EMJH culture medium.