Capacitation In Vitro Maturation (CAPA-IVM) of Human Oocytes.

Oocyte in vitro maturation (IVM) is a patient-friendly technique that generates mature oocytes in vitro from patients who receive minimal or no gonadotrophin stimulation. Although it was discovered a long time ago, the clinical application of IVM has been constrained by its lower success rates compared with conventional IVF, principally due to suboptimal oocyte competency. Recent advancements in the culture system for immature oocytes have significantly enhanced both maturation efficiency and embryo quality. Consequently, this technique is experiencing a resurgence in interest among clinicians and embryologists globally. This chapter details the laboratory protocols employed in a novel form of IVM, called capacitation (CAPA) IVM.