Journal of oral microbiology · 2025
Distinct trajectory of gut microbiota driven by a human oral commensal: insights from a murine study.
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Lin Wei-Ting, Lee Shiao-Pieng, Li Chin, Chang Chia-Bin, Chien Hsiu-Chuan, Wang Jann-Tay, Hsieh Song-Chou, Wu Shu-Fen, Tseng Yu-Chao
Аннотация
BACKGROUND: Oral microbes modulate the gut microbiota. Haemophilus parainfluenzae, a core human oral commensal with immunomodulatory properties, is reduced in autoimmune diseases, while mitigating Sjögren's syndrome-like disease with improved oral microbiota in female NOD mice. However, whether it modulates the gut microbiota remains unknown.
OBJECTIVE: To study the modulatory effect of oral H. parainfluenzae inoculation on the gut microbiota.
DESIGN: Female NOD mice were orally inoculated with H. parainfluenzae following antibiotic treatment. Fecal samples were collected pre- and post-inoculation for 16S rRNA gene sequencing. Splenic antigen-presenting cells were analyzed for systemic immunomodulation.
RESULTS: Despite prominent convergence of diversity and beta dissimilarity within each group, H. parainfluenzae led to distinct core microbiota and overall microbial community. While reducing the Firmicutes-to-Bacteroidetes ratio, H. parainfluenzae enriched Bacteroidaceae and its genus Bacteroides. Bacteroides acidifaciens, a beneficial gut commensal, was enriched in ASV-level analyses. The splenic dendritic cells were reduced. Notably, neither did H. parainfluenzae establish ectopic gut colonization, nor was sustained oral colonization required, indicating that non-viable microbes may be sufficient to direct these responses.
CONCLUSIONS: H. parainfluenzae drives a distinct gut microbiota reconstitution trajectory, characterized by B. acidifaciens enrichment without establishing notable colonizations, supporting its role in the oral-gut axis and warranting future postbiotic research.
OBJECTIVE: To study the modulatory effect of oral H. parainfluenzae inoculation on the gut microbiota.
DESIGN: Female NOD mice were orally inoculated with H. parainfluenzae following antibiotic treatment. Fecal samples were collected pre- and post-inoculation for 16S rRNA gene sequencing. Splenic antigen-presenting cells were analyzed for systemic immunomodulation.
RESULTS: Despite prominent convergence of diversity and beta dissimilarity within each group, H. parainfluenzae led to distinct core microbiota and overall microbial community. While reducing the Firmicutes-to-Bacteroidetes ratio, H. parainfluenzae enriched Bacteroidaceae and its genus Bacteroides. Bacteroides acidifaciens, a beneficial gut commensal, was enriched in ASV-level analyses. The splenic dendritic cells were reduced. Notably, neither did H. parainfluenzae establish ectopic gut colonization, nor was sustained oral colonization required, indicating that non-viable microbes may be sufficient to direct these responses.
CONCLUSIONS: H. parainfluenzae drives a distinct gut microbiota reconstitution trajectory, characterized by B. acidifaciens enrichment without establishing notable colonizations, supporting its role in the oral-gut axis and warranting future postbiotic research.