Inhibitory effects of surface pre-reacted glass-ionomer eluate on Candida albicans and commensal oral streptococci dual-species biofilm.

Surface pre-reacted glass-ionomer (S-PRG) fillers, a bioactive material, possess various biological functions. Various studies have investigated the bioactive effects of S-PRG fillers; however, their effects on biofilms formed by multiple microorganisms remain unclear. Recently, several cases of rampant caries onset and severe caries in childhood caused by coinfections with cariogenic bacteria and commensal oral fungi have been reported. This study evaluated the effects of S-PRG eluate on complex biofilms involved in the onset of rampant and severe caries. The amounts of biofilm formed by co-culturing cariogenic bacteria and fungus was suppressed in a concentration-dependent manner by the S-PRG eluate. Furthermore, intracellular oxidative stress increased in proportion to the S-PRG eluate. The results of this study suggest that oxidative stress generated by S-PRG eluate suppresses the formation of complex microbial biofilms.