Establishment and preliminary application of a rapid detection method for Salmonella LAMP-LFD.

INTRODUCTION

Salmonella is one of the main pathogenic bacteria causing foodborne diseases. This microorganism mainly infects food, especially meat and poultry products that have not been fully heated or cooked, and enters the human body, causing infection, or infects through the handling of contaminated food. Salmonella infection occurs worldwide, highlighting the importance of preventing such diseases. Salmonella disease poses a serious threat to public health security in low- and middle-income countries and even globally. Therefore, it is important to quickly and accurately detect Salmonella. Currently, the detection methods for Salmonella mainly include traditional detection methods as well as immunologic and molecular-based technologies. Traditional methods are cumbersome, time-consuming, and inefficient, and methods based on immunology and molecular biology require advanced equipment and technical know-how.

METHODS

Loop-mediated isothermal amplification (LAMP) has advantage of rapidity, accuracy, and economy, but it is necessary to contact the carcinogen ethidium bromide when observing the results of agarose gel electrophoresis.

RESULTS

In this experiment, a LAMP combined with lateral-flow device (LAMP-LFD) method was established that identified different serotypes of Salmonella.

DISCUSSION: This method is convenient, fast, nontoxic, and harmless. It can detect Salmonella specifically and launch preliminary evaluation and application.