Microbial pathogenesis · 2025
Genomic characterization and antimicrobial resistance profile of Salmonella spp. clinical isolates: A single-centre hospital-based exploratory study from Uttarakhand, India.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Bhatia Mohit, Shamanna Varun, Nagaraj Geetha, Gupta Pratima, Omar Balram Ji, Singh Arpana, Anand Priyal, Gk Harshitha, Ravikumar K L
Аннотация
OBJECTIVES: This single-centre, hospital-based study from Uttarakhand, India, aimed to describe the genomic characterization data of Salmonella spp. isolated from clinical samples.
METHODS: This study was performed between January 2019 and January 2023 and included eighteen clinical Gram-negative bacterial isolates which were identified as Salmonella species as per standard microbiological guidelines. The test isolates were sent to the Central Research Laboratory (CRL), Bengaluru, India, for characterization and whole-genome sequencing (WGS). At CRL, the test isolates were revived and subjected to preliminary identification and antibiotic susceptibility testing (AST) using the VITEK 2 Compact system, the results of which were interpreted according to the Clinical Laboratory Standards Institute (CLSI) guidelines 2023.
RESULTS: Of the 18 Salmonella spp. test isolates, twelve belonged to serovar Typhi, five to serovar Paratyphi A, and one isolate to serovar Typhimurium. The serotyping results were further confirmed by WGS in which no disparity was observed. Genotyping results were determined for twelve S. Typhi isolates, of which 41.7 % (5/12) belonged to genotype 4.3.1.2 (H58 LII) and one isolate belonged to genotype 4.3.1.1 (H58 LI), respectively. All isolates were susceptible to ampicillin, chloramphenicol, co-trimoxazole, ceftriaxone and azithromycin. Ciprofloxacin resistance and intermediate susceptibility were observed in 14 and four isolates, respectively. Genes conferring resistance to fluoroquinolones were detected in all test isolates and were mainly associated with mutations on the gyrA and parC genes. Triple mutants bearing gyrA-S83F, gyrA-D87N, and parC-S80I genes were observed in three isolates belonging to genotype 4.3.1.2.1. Genes encoding resistance to other antimicrobial drug classes were not observed in the dataset. The major Pathogenicity islands, SPI-1, SPI-2, SPI-3, SPI-5 and SPI-9, were detected in all test isolates.
DISCUSSION: This study indicates that Salmonella strains are susceptible to most antibiotics for enteric fever, except fluoroquinolones. However, gathering data from diverse Indian regions is essential to understanding regional variations in Salmonella antimicrobial resistance emergence.
METHODS: This study was performed between January 2019 and January 2023 and included eighteen clinical Gram-negative bacterial isolates which were identified as Salmonella species as per standard microbiological guidelines. The test isolates were sent to the Central Research Laboratory (CRL), Bengaluru, India, for characterization and whole-genome sequencing (WGS). At CRL, the test isolates were revived and subjected to preliminary identification and antibiotic susceptibility testing (AST) using the VITEK 2 Compact system, the results of which were interpreted according to the Clinical Laboratory Standards Institute (CLSI) guidelines 2023.
RESULTS: Of the 18 Salmonella spp. test isolates, twelve belonged to serovar Typhi, five to serovar Paratyphi A, and one isolate to serovar Typhimurium. The serotyping results were further confirmed by WGS in which no disparity was observed. Genotyping results were determined for twelve S. Typhi isolates, of which 41.7 % (5/12) belonged to genotype 4.3.1.2 (H58 LII) and one isolate belonged to genotype 4.3.1.1 (H58 LI), respectively. All isolates were susceptible to ampicillin, chloramphenicol, co-trimoxazole, ceftriaxone and azithromycin. Ciprofloxacin resistance and intermediate susceptibility were observed in 14 and four isolates, respectively. Genes conferring resistance to fluoroquinolones were detected in all test isolates and were mainly associated with mutations on the gyrA and parC genes. Triple mutants bearing gyrA-S83F, gyrA-D87N, and parC-S80I genes were observed in three isolates belonging to genotype 4.3.1.2.1. Genes encoding resistance to other antimicrobial drug classes were not observed in the dataset. The major Pathogenicity islands, SPI-1, SPI-2, SPI-3, SPI-5 and SPI-9, were detected in all test isolates.
DISCUSSION: This study indicates that Salmonella strains are susceptible to most antibiotics for enteric fever, except fluoroquinolones. However, gathering data from diverse Indian regions is essential to understanding regional variations in Salmonella antimicrobial resistance emergence.