Differential infection dynamics in mononuclear and polymorphonuclear cells during Salmonella Typhimurium infection and in vitro exposure to diazoxon.

S. Typhimurium bacteria cause one of the most recurrent gastrointestinal diseases worldwide. This bacterium can settle in the gastrointestinal tract and internalize into different cellular strains, causing the formation of cellular reservoirs that subsequently lead to systemic dissemination. Exogenous factors such as pesticide exposure can also cause immunological alterations, increasing susceptibility to bacterial infection. The present work evaluated the infection capacity of Salmonella Typhimurium, during a short period (1 h) on mononuclear and polymorphonuclear cells previously exposed to diazoxon (1 h, during 4 h). Mononuclear cells were infected more frequently and in greater magnitude than polymorphonuclear cells. However, when actin polymerization and the release of reactive oxygen species (ROS) were analyzed, polymorphonuclear cells showed increased activity. These processes were evidenced by conformational changes during infection. This suggests differential dynamics of S. Typhimurium infection in mononuclear and polymorphonuclear cells previously exposed to diazoxon.