The PARK2_e01(-697) Polymorphism does not Associate with Susceptibility to Typhoid in Punjabi Population, Pakistan: A Case Control Study.

BACKGROUND

SNP based association studies have revolutionized the field of biomedicines. Enteric fever is a systemic disease with etiologic agent Salmonella enterica serovar typhi and paratyphi. It is a serious health issue worldwide and presents wide variations in incidence, rates, and severity. Previous investigations revealed that genetic variations may lead to susceptibility to typhoid fever. The current study was performed to investigate the potential association of PARK2_e01(-697) polymorphism with the susceptibility to typhoid in the Punjabi population.

METHODS

For this case-control study, blood samples obtained from typhoid patients with positive Typhidot or blood culture test (n=72) and healthy controls (n=73) were processed for DNA extraction. The polymorphism PARK2_e01(-697) analysis was carried out by using PCR and RFLP.

RESULTS

No allelic association was found between PARK2_e01(-697) and susceptibility to typhoid fever in the understudy population.

CONCLUSION

This case control study is the demonstration of the non-association of PARK2_e01(-697) with typhoid in the Pakistani population. Future research, using larger population size, will help to elucidate the role of PARK2_e01(-697) polymorphism in typhoid pathogenesis.