Clinica chimica acta; international journal of clinical chemistry · 2003
Tamoxifen might influence the affinity of LPL for heparin-sepharose.
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Kawano Mikihiko, Hozumi Yasuo, Itoh Kouichi
Аннотация
BACKGROUND: We previously reported that the tamoxifen treatment caused a decrease in lipoprotein lipase (LPL) activity and an increase in LPL mass. We hypothesized that tamoxifen may increase the quantity of inactive LPL.
METHODS: Lipoprotein lipase in post-heparin plasma usually exists in both monomeric and dimeric forms, which may be separated on a heparin-Sepharose column with different salt concentrations. Lipoprotein lipase in post-heparin plasma from postmenopausal patients with hypertriglyceridemia treated with or without tamoxifen was incubated with or without 4-hydroxy-tamoxifen (4-OHT), the monomers and dimers were separated on a heparin-Sepharose column and their masses were measured.
RESULTS: The masses of total LPL and dimeric LPL of tamoxifen-treated patients were significantly higher than those of control subjects. Monomeric LPL of tamoxifen-treated patients passed more slowly through the heparin-Sepharose column compared with that of control subjects. The ratio of monomeric LPL to dimeric LPL of tamoxifen-treated patients was 0.61, significantly lower than that of control subjects, which was 1.45 (p<0.01). In addition, monomeric LPL incubated with 4-OHT passed more slowly through the heparin-Sepharose column compared with that incubated without 4-OHT.
CONCLUSION: Tamoxifen influences the affinity of LPL for heparin.
METHODS: Lipoprotein lipase in post-heparin plasma usually exists in both monomeric and dimeric forms, which may be separated on a heparin-Sepharose column with different salt concentrations. Lipoprotein lipase in post-heparin plasma from postmenopausal patients with hypertriglyceridemia treated with or without tamoxifen was incubated with or without 4-hydroxy-tamoxifen (4-OHT), the monomers and dimers were separated on a heparin-Sepharose column and their masses were measured.
RESULTS: The masses of total LPL and dimeric LPL of tamoxifen-treated patients were significantly higher than those of control subjects. Monomeric LPL of tamoxifen-treated patients passed more slowly through the heparin-Sepharose column compared with that of control subjects. The ratio of monomeric LPL to dimeric LPL of tamoxifen-treated patients was 0.61, significantly lower than that of control subjects, which was 1.45 (p<0.01). In addition, monomeric LPL incubated with 4-OHT passed more slowly through the heparin-Sepharose column compared with that incubated without 4-OHT.
CONCLUSION: Tamoxifen influences the affinity of LPL for heparin.