Archives of dermatology · 1993
Rapid diagnosis of coccidioidomycosis with a DNA probe to ribosomal RNA.
Клинический случайСтатьяНазвание и аннотация: Перевод в очереди
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Beard J S, Benson P M, Skillman L
Аннотация
BACKGROUND: Although nucleic acid probe hybridization assays were previously exclusively used as a tool in the research setting, such assays have recently become commercially available for the detection of a variety of infectious microorganisms.
OBSERVATIONS: We used a commercially available DNA hybridization probe test that targets organism-specific ribosomal RNA sequences to rapidly diagnose a patient with disseminated coccidioidomycosis. The natural amplification inherent to such DNA:RNA probe systems obviates the need for electrophoretic separation and amplification steps, which are often required in more traditional DNA:DNA probe assays. With this probe, culture confirmation was obtained within 48 hours after the clinical specimens were received.
CONCLUSION: Rapid DNA hybridization probe techniques have wide application in infectious diseases, especially those characterized by slow culture growth of pathogens such as deep fungi and atypical mycobacteria.
OBSERVATIONS: We used a commercially available DNA hybridization probe test that targets organism-specific ribosomal RNA sequences to rapidly diagnose a patient with disseminated coccidioidomycosis. The natural amplification inherent to such DNA:RNA probe systems obviates the need for electrophoretic separation and amplification steps, which are often required in more traditional DNA:DNA probe assays. With this probe, culture confirmation was obtained within 48 hours after the clinical specimens were received.
CONCLUSION: Rapid DNA hybridization probe techniques have wide application in infectious diseases, especially those characterized by slow culture growth of pathogens such as deep fungi and atypical mycobacteria.