Adsorption of dibutyl phthalate by multi-walled carbon nanotubes aggravates hemogram, liver and kidney damage in rats.

Studies have shown that environmental pollutants, DBP and MWCNTs, are physiologically toxic. To investigate whether MWCNTs with adsorbed DBP would aggravate this toxicity, we exposed rats to this combination via tail vein injection for two weeks. The results showed the numbers of total WBCs, LYMs, MONs, GRAs, RBCs, PLTs were significantly higher, decrease in GSH, and increase in IL-6, TNF-α, and IgE. Tissue sections revealed hepatic steatosis, sinusoidal sludge, and dilated glomerular capillaries filled with erythrocytes. Blood glucose regulation was impaired, and serum TP, ALT, Cr, and BUN levels were elevated. GO and KEGG analyses showed DEPs were mainly enriched in the catabolism of lipids and proteins, hepatobiliary system development, acute inflammatory response, and metabolic pathways. These findings demonstrate that MWCNTs with adsorbed DBP are more toxic, inducing inflammatory factor release and abnormal metabolic protein expression in the liver and kidney through oxidative damage.