Magnetic resonance in medicine · 2026
Using Manganese-Enhanced DCE-MRI to Assess Blood-Cerebrospinal Fluid Barrier Transport.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Perera Charith, Feng Zhiping, Nizari Shereen, Muza Phillip, Katsiva Christina, Kalber Tammy L, Lythgoe Mark F, Harrison Ian F, Stuckey Daniel J, Wells Jack A
Аннотация
PURPOSE: We developed a dynamic, heavily T2-weighted (hT2w) FLAIR protocol to resolve acute Mn2+ transport kinetics, as a proxy for Ca2+, across the choroid plexus (ChP)-cerebrospinal fluid (CSF) interface, in vivo. By quantifying intercompartmental Mn2+ dynamics, and comparing these with Gd-DOTA, we sought to characterize blood-CSF-barrier (BCSFB)-mediated tracer transport, combining high in-plane resolution and 4-min temporal sampling.
METHODS: hT2w-FLAIR DCE MRI was conducted in anesthetized C57BL/6 mice, receiving intravenous Mn2+ or Gd-DOTA. Semi-quantitative tracer kinetic modeling permitted regional and inter-tracer comparisons.
RESULTS: Mn2+ rapidly crossed from the vasculature into the CSF, across the ChP, with similar arrival times and time-to-peak across compartments. Mn2+ delivery into the ChP exhibited a 5.7-fold steeper uptake rate (p = 0.031) and a 5.6-fold higher peak signal amplitude (p = 0.0028) than in the LV-CSF. In contrast, Gd-DOTA exhibited a 2.2-fold shorter ChP plateau duration (p = 0.034) and 2.2-fold faster ChP clearance rate (p = 0.0047), compared to Mn2+. Within the CSF, there were significant temporal delays with Gd-DOTA relative to Mn2+: a lengthened arrival (eight-fold, p = 0.045) and time-to-peak (six-fold, p = 0.024).
CONCLUSION: Our hT2w-FLAIR DCE-MRI approach captured the early BCSFB transport window for Mn2+, providing distinct ChP and LV-CSF kinetics at a higher temporal resolution and with reduced CSF signal contamination compared to prior approaches. These findings highlight that MRI tracer choice strongly shapes measured BCSFB kinetics, and supports the use of Mn2+ as a tool for probing compartment-specific ChP transport.
METHODS: hT2w-FLAIR DCE MRI was conducted in anesthetized C57BL/6 mice, receiving intravenous Mn2+ or Gd-DOTA. Semi-quantitative tracer kinetic modeling permitted regional and inter-tracer comparisons.
RESULTS: Mn2+ rapidly crossed from the vasculature into the CSF, across the ChP, with similar arrival times and time-to-peak across compartments. Mn2+ delivery into the ChP exhibited a 5.7-fold steeper uptake rate (p = 0.031) and a 5.6-fold higher peak signal amplitude (p = 0.0028) than in the LV-CSF. In contrast, Gd-DOTA exhibited a 2.2-fold shorter ChP plateau duration (p = 0.034) and 2.2-fold faster ChP clearance rate (p = 0.0047), compared to Mn2+. Within the CSF, there were significant temporal delays with Gd-DOTA relative to Mn2+: a lengthened arrival (eight-fold, p = 0.045) and time-to-peak (six-fold, p = 0.024).
CONCLUSION: Our hT2w-FLAIR DCE-MRI approach captured the early BCSFB transport window for Mn2+, providing distinct ChP and LV-CSF kinetics at a higher temporal resolution and with reduced CSF signal contamination compared to prior approaches. These findings highlight that MRI tracer choice strongly shapes measured BCSFB kinetics, and supports the use of Mn2+ as a tool for probing compartment-specific ChP transport.