Cross-Species Translation of Drug-Induced Toxicity in the Small Intestine Using Mathematical Modeling.

We previously detailed a quantitative systems toxicology modeling framework for early prediction of chemotherapeutic-induced clinical intestinal adverse effects. The model describes stem and progenitor cell dynamics in the small intestinal epithelium and integrates epithelial-related processes, such as transcriptional profiles, citrulline kinetics, and the probability that the organism will have diarrhea. This protocol describes a step-by-step guide to the examples detailed in the original manuscript, namely 5-FU- and doxorubicin-induced intestinal toxicity.