Isolation of Nucleic Acids from Aspergillus fumigatus for Sequencing.

Invasive aspergillosis is a severe fungal infection that usually affects the immunocompromised, e.g., transplant patients. Sequence-based analysis has become increasingly important for understanding fungal virulence, fungal identification, and monitoring drug resistance. In this chapter, we describe methods for the isolation of fungal DNA from different sample types and quality control of sequence data.