Dataset of RNA-seq profiles from THP-1-derived macrophages pretreated with AZD7648 and stimulated with CT DNA.

This article describes an RNA-seq dataset generated from THP-1-derived macrophages under four treatment conditions: untreated control, DNA-dependent protein kinase catalytic subunit (DNA-PKcs) inhibitor AZD7648 alone, calf thymus DNA (CT DNA) alone, and AZD7648 pretreatment followed by CT DNA stimulation. Each condition included three biological replicates, yielding 12 transcriptome samples in total. Libraries were sequenced on the Illumina NovaSeq 6000 platform using paired-end 150-bp reads. The dataset includes raw sequencing reads, gene-level expression matrices, and associated quality control metrics. All RNA samples exhibited RIN values between 9.5 and 9.9. Clean reads ranged from 54.83 to 70.25 million per sample, with Q30 values of 97.18%-97.34%, total mapping rates of 98.24%-98.51%, and unique mapping rates of 95.55%-95.81%. This dataset provides a resource for comparative transcriptomic analyses of cytosolic DNA stimulation and DNA-PKcs inhibition in macrophage cells and may support reuse in studies of innate immune signaling, cytosolic DNA sensing, and pathway-focused reanalysis.