Медицинская библиотека · Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology · 2001

[Two virus strains of isolated in China are recombinant alphaviruses].

He H, Lu X, Yang Y

Аннотация на английскомСтатьяПоддержка исследования: вне правительства СШАПеревод в очереди

Аннотация

OBJECTIVE

To determine the classification, phylogenetic and genetic type of XJ-90260 and XJ-91006 viruses isolated in China.

METHODS

Two pairs of specific primers were designed to amplify the NSP4 and E1-3' UTR genes of XJ-90260 and XJ-91006 viruses and the nucleotide sequences between them and with that of other Alphavirus were compared.

RESULTS

The nucleotide sequences identity between XJ-90260 and XJ-91006 viruses was 100% and has showed the highest with WEE, comparing with the other 26 Alphaviruses in 3'-UTR. NSP4 gene of XJ-90260 and XJ-91006 viruses high homologous with EEE virus and E-1 gene high homologous wih SIN virus. XJ-90260 and XJ-91006 viruses belong to the B group of WEE and are closest to the Russian strain (Y62-33) by phylogenetic analysis.

CONCLUSION

XJ-90260 and XJ-91006 viruses isolated in China are recombinant Alphaviruses, they belong to the same genetic type as WEE does.

Для цитирования: He H, Lu X, Yang Y. [Two virus strains of isolated in China are recombinant alphaviruses].. Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology. 2001.

Предметные рубрики MeSH

Alphavirus Animals Base Sequence Cells, Cultured Encephalomyelitis, Western Equine Phylogeny Sequence Homology, Nucleic Acid

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