Effect of methanolic extract of Salix babylonica on biochemical parameters and gene expression in streptozotocin induced diabetic rat model.

BACKGROUND

Diabetes Mellitus (DM) is a metabolic syndrome characterized by hyperglycemia and associated with defects in insulin production and action.

AIM: This study aimed to delineate the antidiabetic potential of Salix babylonica.

METHODS

Streptozotocin (55 mg/kg) was used to develop a subacute model of diabetes in Sprague-Dawley rats. Ten days later, fasting and random blood glucose levels, water and food intake, and body weight (BW) were recorded. Serum insulin levels were examined to ensure the induction of diabetes mellitus. The effects of oral administration of MESB at doses of 250, 500, and 750 mg/kg on body weight; fasting and postprandial hyperglycemia; oral glucose tolerance test (OGTT); serum concentrations of HbA1c, adiponectin, amylase, tumor necrosis factor-α (TNF-α), catalase, and superoxide dismutase (SOD); histopathological changes in the pancreas, liver, and kidney; and mRNA expression of TNF-α, SIRT1, and SIRT2 were studied over 28 days.

RESULTS

MESB significantly improved FBG, RBG, BW, polyphagia, polydipsia, and glucose tolerance in a preclinical DM model. In addition, MESB significantly decreased the serum HbA1c and TNF-α levels. However, the serum adiponectin, amylase, catalase, and SOD levels increased substantially. Moreover, histopathological analysis showed that MESB-corrected DM induced pathological changes in the organs of the diabetic rats. Finally, molecular analysis revealed that MESB significantly reduced the overexpression of the TNF-α transcript, whereas the mRNA expression of SIRT1 and SIRT2 significantly increased in diseased animals.

CONCLUSION

Overall, the findings highlighted that Salix babylonica extract could be a successful therapeutic candidate for ameliorating DM.