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Unusual presentation of pulmonary inflammatory pseudotumor (plasma cell granuloma) as esophageal obstruction.

A 12-year old boy had a five-year course of dysphagia secondary to lower esophageal obstruction by a posterior mediastinal extension of an inflammatory pseudotumor of the lung. The case illustrates the capacity of pseudotumor to behave in a locally aggressive manner and to present in extrapulmonic sites.

An 'artificial beta cell' for control of diabetes mellitus: effect on plasma glucagon levels.

We have investigated the use of a glucose-controlled insulin infusion system, or artificial beta cell. On the feedback day, mean plasma glucose was significantly effect of improved control on plasma glucagon levels. Five insulin-requiring diabetic subjects in stable control were hospitalized for two 24 h periods. During one, they were given their usual dose(s) of subcutaneous insulin. In the other, the 'feedback' day, insulin administration was under feedback control by the artificial beta cell. One the feedback day, mean plasma glucose was significantly-lower in all subjects. Variability in p

Plasma cell neoplasia with osteosclerotic lesions. A study of five cases and a review of the literature.

Sixty-eight patients with plasmacytic neoplasia and osteosclerotic lesions were analyzed. Men predominated in this series. Mean age was 55.3 years and 26 patients were younger than 51 years at diagnosis. Early onset of disease was statistically different from multiple myeloma in general. Thirty patients had peripheral polyneuropathy and often neurological manifestations preceded other symptoms. Skeletal pain was less common, whereas hepatomegaly, splenomegaly, and lymphadenopathy were more common than in myeloma in general. Incidence of azotemia, hypercalcemia, high ESR, and anemia was lower t

Complement activation and phagocytosis of cryoglobulin particles in a patient with plasma cell proliferative disease.

The serum of a patient with a non-aggressive plasma cell proliferative disorder contained two monoclonal immunoglobulins: IgG3 lambda in moderate concentration and having cryoglobulin features, and IgA kappa in low concentration and without cryoprecipitability. The patient's serum had low complement concentration and C3 was partly converted into split products in vivo. Complement (C3) together with cryoglobulin and fibrinogen was found by immunofluorescence in sections from skin showing vasculitis. The cryoglobulin particles which formed at room temp. were vividly phagocytized in vitro by neut

Endobronchial plasma cell granuloma (xanthomatous pseudotumor); a light and electron microscopic study.

Plasma cell granuloma (xanthomatous pseudotumor) is polymorphic at light as well as electron microscopic examination. At light microscopy the endobronchial variant of this entity was rich in plasma cells and interwoven, whorl-like, or concentrically arranged spindle cells. Foamy histiocytes and macrophages usually abundant in the intrapulmonary variant were rare. At electron microscopy particles 20 to 50 nm. in size were found at the bronchial mucosal surface but not elsewhere in the lesion. Plasma cells near the bronchial surface contained cytoplasmic fibrils, mitochondria with concentric cri…

The bone marrow in multiple myeloma: correlation of plasma cell ultrastructure and clinical state.

The bone marrow plasma cells of 52 patients with various kinds of monoclonal gammopathies were studied by electron microscopy, and compared to the bone marrow plasma cells of 22 patients with reactive plasmacytosis. Virtually every marrow from patients with myeloma and macroglobulinemia contained plasma cells with disparity between the nuclear maturation and cytoplasmic differentiation. This asynchronous development was not present in plasma cells of reactive marrows nor in plasma cells from patients with megaloblastic anemias. The degree of asynchrony observed in myeloma and macroglobulinemia…

Plasma exosomes from children with juvenile dermatomyositis are taken up by human aortic endothelial cells and are associated with altered gene expression in those cells.

BACKGROUND: The pathology of juvenile dermatomyositis (JDM) is characterized by prominent vessel wall and perivascular inflammation. This feature of the disease has remained unexplained and under-investigated. We have hypothesized that plasma exosomes, which play an important role in inter-cellular communication, may play a role in the vascular injury associated with JDM. OBJECTIVE: To characterize the circulating exosomes of children with JDM and determine whether the small RNA cargoes within those exosomes are capable of altering transcriptional programs within endothelial cells. DESIGN/ME

Development, validation, and implementation of the antibody-secreting cell maturity index: Universal prediction of human plasma cell maturity.

Defining the maturity of long-lived antibody-secreting cells (ASCs) is important for vaccine optimization and research into autoimmune diseases, but current assessment of plasma cell maturity is limited. We developed a universal, robust method to define plasma cell maturity using a meta-analysis of public, human cytometry data, with harmonized expression of ASC maturity markers across samples. Vaccination or infection samples were selected to train a random forest-based machine learning prediction model (performance of r = 0.866). After evaluation (performance of r = 0.616), the final model, n

De novo pyrimidine synthesis controls germinal center B cell and plasma cell fates and systemic autoimmunity.

Whether and how pyrimidine metabolites promote systemic autoimmunity is unknown. Here, metabolomics and 15N-amide glutamine tracing show enhanced flux through de novo pyrimidine synthesis in systemic lupus erythematosus (SLE)-prone B cells. Temporal inhibition of pyrimidine synthesis dampens SLE-prone but not foreign antigen-specific germinal center (GC), plasma cell (PC), and antibody responses. Uridine monophosphate synthase (UMPS) conditional deletion, however, reveals a B cell-intrinsic requirement of de novo pyrimidine synthesis in foreign antigen-driven and SLE-prone GC, PC, and antibody

Automated B-cell and plasma cell identification using unsupervised clustering by FlowSOM and an excel-based classification model.

Manual gating for plasma cell (PC) identification in multiparametric flow cytometry (MFC) is time-consuming and operator-dependent, especially when PCs are scarce. Artificial intelligence approaches such as unsupervised clustering (e.g., FlowSOM) map high-dimensional data that still require expert interpretation. The primary aim of this work was to develop a lightweight, transparent, spreadsheet-based algorithm for a classification model that integrates with automated clustering outputs for standardized B-cell and PC identification in research flow cytometry datasets. Bone marrow aspirates wer

Comparison of Plasma- and Adipose Tissue-Derived Stromal Cell Extracellular Vesicles for Schwann Cell Proliferation and Cost-Effectiveness.

BACKGROUND: Preparation of Extracellular vesicles (EVs) from cell cultures is labor-intensive. Plasma-derived EVs have demonstrated proliferative effects. The aim of this study was to compare the proliferative effect of plasma EVs on Schwann cells (SC) and to assess the cost structure of EVs derived from plasma versus Adipose tissue-derived stromal cells (AdSCs). METHODS: EVs isolated from rat platelet-rich plasma (PRP), platelet-poor plasma (PPP), and AdSC cell culture were assessed by quantity, size, and phenotypic markers. Both plasma-EVs in three dosages were added to primary rat SC cultu

Translating B-Cell and Plasma-Cell Targeting from Oncology to Autoimmunity: Modalities, Quantitative Bridging, and a Development Roadmap.

B cells drive B-cell malignancies and diverse autoimmune diseases through autoantibody production, antigen presentation, and cytokine dysregulation, and persistence of pathogenic B-cell or plasma-cell compartments. Across autoimmune diseases, the dominant pathogenic compartment varies, ranging from precursor-dependent states to tissue-compartment inflammation and long-lived plasma cell-weighted autoantibody production. This review synthesizes the biological rationale for B-cell-axis targeting across oncology and autoimmunity and proposes a pathobiological framework spanning precursor-dependent

Plasma cell identity escape drives resistance to anti-BCMA T-cell-redirecting therapy in multiple myeloma.

Chimeric antigen receptor T-cell (CART) and T-cell engager (TCE) therapies targeting B-cell maturation antigen (BCMA) are transforming the treatment landscape for relapsed multiple myeloma (MM). However, despite impressive initial response rates, most patients eventually relapse. To investigate this unmet medical need, we applied whole-genome sequencing (WGS) to MM cells from cohorts of 102 relapsed patients treated with anti-BCMA CART and TCE therapies. Several genomic alterations were associated with clinical outcomes, particularly primary refractoriness, including high genomic complexity an

Воздействие остаточных клональных плазменных клеток в фазе S во время аутологической трансплантации стволовых клеток на клинические результаты.

Автологическая трансплантация стволовых клеток (ASCT) является ключевой терапевтической стратегией для многих пациентов, у которых диагностируется миелома (ММ), однако ранние рецидивы после трансплантации остаются серьезной клинической проблемой. Испытание на распространение плазменных клеток (ПХПРО), в ходе которого определяется доля клональных плазменных клеток в костном мозге в фазе S-фазе (S-фаза %), представляет собой масштабируемую альтернативу измерению их скорости распространения по сравнению со старым индексом плазменных клеток (PCLI). Воздействие S-фазы в остаточных клональных плазме

Совпадение в аннотации оригинала
Autologous stem cell transplantation (ASCT) is a key therapeutic strategy for many patients diagnosed with multiple myeloma (MM), yet early relapses post-transplant remains a major clinical challenge. The plasma cell proliferation (PCPRO) test, which quantifies the proportion of clonal plasma cells in the bone marrow in S-phase (S-phase%) offers a scalable a…

Spatially and Clonally Multiple B-/Plasma Cell Proliferations in T Follicular Helper Cell Lymphoma: A Case Report.

T follicular helper cell lymphoma (TFHL) is a systemic T-cell lymphoma with a phenotype reminiscent of TFH cells and frequent RHOA p.G17V mu … ral pleural effusions developed. Cytology of the pleural effusion revealed abnormal lymphocytes and plasma cells. The abnormal T-cells were positive for CD3, CD4, CD10, and PD1, while the neoplastic plasma cells showed kappa-predominant light-chain restriction. Additional cutaneous biopsies from the left forearm and upper arm revealed Epstein-Barr virus-positi …

Case Report: Angioimmunoblastic T-cell lymphoma with coexisting plasma cell tumors: three cases and review of the literature.

Angioimmunoblastic T cell lymphoma (AITL) is classified as a nodal T-cell lymphoma from T follicular helper(Tfh) cells. Dysfunctional Tfh ce … riven immune dysregulation and plasma-cell proliferation. Case analysis showed that some monoclonal plasma cells originated from the interior of AITL, while others originated from the bone marrow. This indicated that the evolution of plasma cell tumors may arise from either the lymphoma microenvironment or the systemic immune status. In cases of coexistence of …

Machine learning reveals distinct T-cell receptor clusters in plasma cell dyscrasias compared to healthy controls.

T-cell receptor (TCR) repertoire diversity has been implicated in the progression and prognosis of multiple myeloma (MM). This study aimed to evaluate the association between T-cell clonality, immune response, and clinical outcomes in patients with plasma cell dyscrasias using next-generation sequencing of the TCR β chain (TCRB). TCRB sequencing was performed on peripheral blood samples from patients with monoclonal gammopathy of undetermined significance (MGUS), smoldering multiple myeloma (SMM), and newly diagnosed multiple myeloma (MM). Healthy individuals served as controls. No significant