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Effect of mitochondrial protein synthesis inhibitors on the trophic action of nerve stump in mice.

We studied the effect of mitochondrial protein synthesis inhibitors on the neurotrophic action of the nerve stump in mice. The sciatic nerve was cut as close to, or as far from the extensor digitorum longus muscles as possible. At 2 and 3 days after denervation, depolarization in the resting membrane potential of muscles with long nerve stumps (14-16 mm) was significantly smaller than in muscles with very short (less than 2 mm) nerve stumps. Chloramphenicol (100 mg/kg, p.o.), erythromycin (4 micrograms/kg, i.p.), ethidium bromide, (10 micrograms/kg, i.p.) or acridine (10 micrograms/kg, i.p.), …

Accumulation of lankamycin derivative with a branched-chain sugar from a blocked mutant of chalcose biosynthesis in Streptomyces rochei 7434AN4.

Lankamycin, a macrolide antibiotic produced by Streptomyces rochei 7434AN4, exhibits a moderate antimicrobial activity and acts as a synergistic pair with carbocyclic antibiotic lankacidin C by binding to the ribosome exit tunnel. Its biosynthetic gene (lkm) cluster (orf24-orf53) is located on the largest plasmid pSLA2-L (210,614 bp). Our group possesses a variety of lankamycin derivatives and macrolide-modification enzymes including P450 enzymes and glycosyltransferases, which may lead to expand the chemical library of bioactive macrolides. Here we constructed a mutant of a 3-ketoreductase ge

Synthetic anaplerotic modules for the direct synthesis of complex molecules from CO2.

Anaplerosis is an essential feature of metabolism that allows the continuous operation of natural metabolic networks, such as the citric aci … nd validated our designs by producing 6-deoxyerythronolide B (6-DEB), the C21-macrolide backbone of erythromycin. Our best design allowed the carbon-positive synthesis of 6-DEB via 54 enzymatic reactions in vitro at yields comparable to those with isolated 6-DEB polyketide synthase (DEBS). Our work showcases how new-to-nature anaplerotic modules can be designe …

Genetic and biochemical analysis of the antibiotic biosynthetic gene clusters on the Streptomyces linear plasmid.

We extensively analyzed the giant linear plasmid pSLA2-L in Streptomyces rochei 7434AN4, a producer of two structurally unrelated polyketide antibiotics, lankacidin and lankamycin. It was found that amine oxidase LkcE oxidizes an acyclic amine to an imine, which is in turn converted to the 17-membered carbocyclic lankacidin. Heterologous expression and translational fusion experiments indicated the modular-iterative mixed polyketide biosynthesis of lankacidin. Concerning to lankamycin biosynthesis, starter unit biosynthesis and the post-PKS modification pathway were elucidated by feeding and g

Infectious mononucleosis presenting as erythema multiforme.

Infectious mononucleosis is rarely associated with skin lesions. A 23-year-old woman with acute infectious mononucleosis who presented with moderately severe erythema multiforme is reported. The role of steroids in the management of this condition is briefly discussed.

The clinical spectrum of bacillary angiomatosis.

Bacillary angiomatosis is a recently recognized bacterial infectious disease that is seen mainly in patients with the acquired immunodeficiency syndrome. Including this publication, 45 patients have been described in the medical literature. In this report we describe examples of the clinical presentations of bacillary angiomatosis and review therapeutic strategies.

[Legionella infections--an underestimated disease group].

As in other countries, pneumonia due to Legionella bacteria occurs in Denmark. Even so, legionellosis is rarely diagnosed and often late in the course of illness. This may be due both to the variable and nonspecific clinical picture of the infection, and to the fact that currently available diagnostic methods are inadequate in a clinical context. Even in severe cases, the prognosis of Legionnaires' disease is quite good, if adequate antibiotic therapy is instituted early in the course of illness. The treatment of Legionnaires' disease differs from the treatment of most other pneumonias; freque

[Massive blenorrhea in a newborn infant caused by a Chlamydia trachomatis subtype].

An 8-day-old baby girl was found to be suffering from massive blennorrhoea caused by a strain of Chlamydia trachomatis. Differential diagnosis included possible bacterial (e.g. Pseudomonas, Staphylococci and Haemophilus sp.) and viral causes of the disease. A 10-day systemic therapy with erythromycin 50 mg/kg/body weight is sufficient to prevent possible complications such as pneumonia. The parents also have to be treated for this disease.

[Phylotyping and Determining the Antimicrobial Susceptibility of Cutibacterium acnes Isolated from Patients with Acne Vulgaris].

Cutibacterium acnes (formerly known as Propionibacterium acnes), obligate anaerobic gram-positive diphtheroid, is a member of normal skin mi … reaction (PCR) using specific primers. The minimum inhibitory concentrations (MICs) of clindamycin, erythromycin, azithromycin, tetracycline and doxycycline were determined by agar dilution technique recommended by Clinical and Laboratory Standards Institute (CLSI) for anaerobic bacteria. The majority of the isolates (patient; n= 47, control; n= 47) in both of …

In vitro antimicrobial susceptibility of Alloscardovia omnicolens and molecular mechanisms of acquired resistance.

All the 31 isolates of Alloscardovia omnicolens exhibited low MICs for β-lactams, glycopeptides, linezolid, tetracyclines, and cotrimoxazole. One strain showed MICs ≥256μg/mL for both erythromycin and clindamycin with a single point mutation in 23S rRNA. One strain likely had acquired fluoroquinolone resistance associated with a unique mutation in ParC.

Воздействие антибиотического лечения на активную трахому и окулярную хламидиозную трахоматию.

Антибиотики являются одним из четырёх элементов стратегии SAFE по борьбе с трахомой, которая является причиной большего числа случаев слепоты, чем любая другая болезнь, помимо катаракты. В настоящем документе рассматриваются данные об использовании тематического тетрациклина и перорального тетрациклина, доксициклина, эритромицина, котримоксазола и азитромицина в трахоме, а также ряд вопросов, включенных в число приоритетных направлений исследований и политики.

Совпадение в аннотации оригинала
Antibiotics are one of four arms of the SAFE strategy for the control of trachoma, an eye infection that is responsible for more cases of blindness than any condition other than cataract. The evidence for the use of topical tetracycline and oral tetracycline, doxycycline, erythromycin, cotrimoxazole and azithromycin in trachoma are reviewed here and a number…