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False aminoglycoside resistance in Enterobacterales and non-fermenters by an automated testing system: a descriptive study.

Antimicrobial sensitivity data are important to guide antimicrobial therapy. In microbiological laboratories, routine sensitivity measurements are typically performed with automated testing systems such as VITEK2 and Phoenix. Using data from the Dutch national surveillance system for antimicrobial resistance over a 6-year period, we found that the measured minimum inhibitory concentrations for aminoglycosides in Enterobacterales and non-fermenters were too high for the Phoenix system. In addition, we observed a yearly increase in resistance for several species measured by Phoenix. These findin

Influences of Vibrio cholerae Lipid A Types on LPS Bilayer Properties.

Lipopolysaccharides (LPS) present in the outer leaflet of Gram-negative bacterial outer membranes protect the bacteria from external threats and influence antibiotic permeability as well as immune system recognition. The structure of lipid A, the anchor of an LPS molecule to the outer membrane, can make direct influences on membrane properties. Particularly, in Vibrio cholerae, a Gram-negative bacterium responsible for cholera, a severe diarrheal disease, modifications of lipid A structures grant antibiotic resistance and are a primary factor that led to the current cholera pandemic. However, …

[Comparative study of binding power of polymyxin B and its simulating peptide to lipopolysaccharides lipoid A].

OBJECTIVE: To observe the binding power of polymyxin B (PMB) and its simulation peptide to lipopolysaccharide (LPS) and lipoid A. METHODS: LPS and lipoid A were separately coated on biosensor. 5 microl of PMB (0.01 microg/L) 5 microl of its simulating peptide 1 (PMBSP1 0.01 microg/L) and 5 microl of its simulating peptide 2 (PMBSP2, 0.01 microg/L) were respectively added into the hydrophobic sample pool. The combining power of PMB and its simulating peptides PMBSP1 and PMBSP2 to LPS and lipoid A was compared. RESULTS (1) PMBSP1 almost did not bind LPS and lipoid A, while PMB and PMBSP2 posses

MEN-10700 menarini.

Menarini is investigating the penem beta-lactam antibiotic, MEN-10700, for the potential control of bacterial infections. MEN-10700 compares favorably with other oral beta-lactams against Haemophilus influenzae strains as well as other respiratory pathogens [341073]. The compound has also showed activity against multiple drug-resistant Gram-negative pathogens [341074]. MEN-10700 has a broad spectrum of activity against Gram-positive and Gram-negative bacteria and demonstrates a potency greater than that of imipenem [174404].

Isoprenoid quinones of "Afipia" spp.

The isoprenoid quinone contents of seven strains of "Afipia felis," the type strains of "A. clevelandensis" and "A. broomeae," and reference strains of three unnamed "Afipia" genospecies were determined by reverse-phase high-performance liquid chromatography. The quinone profiles of all "Afipia" strains were essentially identical, with ubiquinone 10 as the major component. The identity of ubiquinone 10 was confirmed by mass spectrometry.

Bacteriological Profile and Antibiotic Susceptibility Patterns of Late-Onset Neonatal Sepsis in Levels III and IV Neonatal Intensive Care Units.

Late-onset sepsis (LOS), which occurs 72 hours after birth, remains an important cause of mortality and morbidity in the neonatal intensive … (75%), with coagulase-negative Staphylococcus (CoNS) being the most common bacteria in both units. Gram-negative bacteria were more frequently isolated from the level IV NICU (36.2%) compared to the level III NICU (8.8%). Escherichia coli (E. coli) and Enterobacter sp. were the most frequently isolated gram-negative bacteria. All gram-positive bacteria were susceptible …

Этап II - испытание параллельной химиорадиотерапии с использованием L-аспарагиназы и MIDLE химиотерапии для недавно диагностированной стадии I/II экстранодал NK/T-клеток лимфомы носового типа (CISL-1008).

Всего 372 Иксоды рицины тикают101 женщины, 122 мужского пола; и 149 Нимфы), собранные с помощью пометок 6 В зависимости от культуры были изучены смешанные лесные массивы восточной Польши на предмет наличия внутренних отрицательных бактерий, помимо бурдорфери Борелия. Галочка для взрослых была изучена в пулах 2 Каждый образец и каждый имфл были изучены в пулах 3-5 Каждый образец. Тики были дезинфицированы в 70 % этанол и гомогенизированный 0.85% NaCl. Разбавленный гомогенат был привит на 3 Виды агарной среды: буферная добыча дрожжей из древесного угля (BCYE-alpha) для изоляции стойко-отрицатель

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A total of 372 Ixodes ricinus ticks (101 females, 122 males, and 149 nymphs) collected by flagging in 6 mixed woodlands of eastern Poland were examined by culture for the presence of internal Gram-negative bacteria other than Borrelia burgdorferi. Adult ticks were examined in pools of 2 specimens each and nymphs were examined in pools of 3-5 specimens each. …

Microbiological quality assessment of potential pathogenic bacteria and multidrug resistance patterns in commercial electrolyte drinks in Dhaka, Bangladesh.

Local electrolyte drinks in Bangladesh are produced without approval from the Bangladesh Standards and Testing Institution (BSTI). Hence, th … screened for antimicrobial susceptibility. Multidrug resistance varied among all Gram-positive and Gram-negative bacterial species. The highest level of resistance was seen in broad-spectrum antibiotics (amoxicillin, ampicillin, ceftriaxone, cefotaxime, and cefepime). About 28% of Gram-positive and 19% of Gram-negative bacteria were hemolytic. Coagulase was found in 34 …

Quorum quenching pathoblockers from bacteria: an alternative approach for bacterial infection management.

Antibiotic resistance in bacterial pathogens has reached a crisis stage and has led to fears of a “post-antibiotic era”. To combat this, cur … sistance. Gram-positive bacteria utilise peptide derivatives of these signalling molecules, whereas Gram-negative bacteria employ fatty acid derivatives. Majority of bacteria utilise both types to regulate expression of target gene. Recent research has prioritised identifying several compounds from diverse sources that can hinder the signalling systems responsible for b …

Rapid pathogen identification directly from positive blood cultures using AUTOF MS1000 MALDI-TOF MS compared to conventional methods: A prospective observational study.

This prospective study, conducted at Al Amiri Hospital, Kuwait, between July and November 2024, assessed the AUTOF MS1000 MALDI-TOF MS syste … , achieving 100 % concordance and species-level identification accuracy for Gram-positive bacteria, Gram-negative bacteria, and yeasts, with a TAT of <30 min-significantly faster than the 24-48 h required by traditional methods. This swift identification process facilitates quicker, more targeted antimicrobial therapy, potentially improving therapeutic outcomes. The stu …

Trends in Bacterial Distribution and Antimicrobial Resistance in Intensive Care Units of Hubei Province, China: A Four-year Surveillance Study (2020-2023).

OBJECTIVE: To investigate the distribution characteristics of common bacteria and changes in antimicrobial resistance in intensive care unit … .5% (3190/15 585), 21.2% (4089/19 258), 21.6% (5067/23 423) and 21.6% (4 831/22 395), respectively. Gram-negative bacteria accounted for 79.5% (12 395/15 585), 78.8% (15 169/19 258), 78.4% (18 356/23 423) and 78.4% (17 564/22 395) of the bacteria, respectively. The top 5 isolates of gram-positive bacteria were Staphylococcus aureus, Enterococcus faecium, Streptococcus p …

Diversity of endophytic bacteria with antimicrobial potential isolated from marine macroalgae from Yacila and Cangrejos beaches, Piura-Peru.

Endophytic bacteria found in marine macroalgae have been studied for their potential antimicrobial activity, consequently, they could serve … ccus faecalis, Staphylococcus epidermidis, S. aureus, and Listeria monocytogenes), and 12 inhibited gram-negative bacteria (Escherichia coli and Salmonella enteric sv typhimurium). Bacteria with better activity belong to Bacillus sp., Kluyvera ascorbata, Pantoea agglomerans, Leclercia adecarboxylata, and Enterobacter sp., which only four showed antifungal activities aga …

Medical Evaluation of the Antimicrobial Activity of Rose Oil on Some Standard Bacteria Strains and Clinical Isolates.

INTRODUCTION: Considerable interest has developed concerning the alternative utilization of aromatic plants rich in essential oils as antiba … hus, it was determined that rose oil could exhibit antimicrobial activity in both Gram-positive and Gram-negative bacteria. DISCUSSION/CONCLUSIONS: Different percentages of diluted solutions of rose oil might be used as a preventive and therapeutic treatment for infections caused by E coli, B subtilis, and S aureus, provided that this is supported by evidence from clin …

In vitro antibacterial activities of DQ-113, a potent quinolone, against clinical isolates.

The antibacterial activity of DQ-113, formerly D61-1113, was compared with those of antibacterial agents currently available. MICs at which 90% of the isolates tested are inhibited (MIC90s) of DQ-113 against clinical isolates of methicillin-susceptible and -resistant Staphylococcus aureus and methicillin-susceptible and -resistant coagulase-negative staphylococci were 0.03, 0.008, 0.03, and 0.06 microg/ml, respectively. Moreover, DQ-113 showed the most potent activity against ofloxacin-resistant and methicillin-resistant S. aureus, with a MIC90 of 0.25microg/ml. DQ-113 inhibited the growth of

The threat of resistance to the new oral cephalosporins.

Phenotypic resistance to beta-lactam antibiotics is mainly due to the prevalence of beta-lactamases. The most common enzymes responsible for resistance to aminopenicillins and older (1st generation) cephalosporins are the TEM-1, TEM-2, OXA-1 and SHV-1 enzymes, all of which are plasmid-mediated. The recent development of oral cephalosporins sharing structural features and an antimicrobial spectrum comparable to that of 3rd-generation cephalosporins has provided highly active compounds for the management of outpatient infections. Two principal mechanisms, however, may cause resistance to these n

Shifting trends in bacterial keratitis in Toronto: an 11-year review.

OBJECTIVE: To review the distribution, current trends, and resistance patterns of bacterial keratitis isolates in Toronto over the last 11 y … 6). The most common isolate overall was coagulase-negative Staphylococcus (CNS) and the most common Gram-negative bacteria isolated was Pseudomonas aeruginosa. Methicillin-resistant Staphylococcus aureus (MRSA) was present in 1.3% of the S aureus isolates, whereas methicillin-resistant CNS (MRCNS) was present in 43.1% of the CNS isolates. There was a trend toward increa …

Microbiological analysis of epidermal growth factor receptor inhibitor therapy-associated paronychia.

BACKGROUND: Paronychia is a well-known, but difficult to treat cutaneous toxicity associated with epidermal growth factor receptor (EGFR) in … etuximab. RESULTS: We identified 20 different species, among these 72% Gram-positive bacteria, 23% Gram-negative bacteria and 5%Candida species. About half of the microbes identified may be considered as residential bacterial flora of the skin, but isolation of microbes from paronychia may indicate a pathogenic relevance for this type of reaction. Eight of our patients …

Postoperative mediastinitis in children: epidemiology, microbiology and risk factors for Gram-negative pathogens.

BACKGROUND: Mediastinitis, although an infrequent complication of median sternotomy, represents a significant source of morbidity and mortality. OBJECTIVE: To determine the incidence and describe the epidemiology and microbiology of mediastinitis in children after cardiac surgery and to identify risk factors for the development of Gram-negative mediastinitis. STUDY DESIGN: This was a retrospective case-control study nested within the cohort of children, birth to 18 years of age, undergoing median sternotomy between January 1, 1995 and December 31, 2003. RESULTS: Forty-three cases of mediast

Ejaculate for Microbiological Culture: To Wash or Not To Wash?

Bacteria can be associated with male infertility. Antibacterial substances (e.g., zinc-containing proteins, antimicrobial peptides) in ejaculates might impair the growth of bacteria in culture. We therefore wanted to test if removing antibacterial substances by washing the ejaculate could improve the detection of bacteria in culture. All ejaculates from patients ≥18 years old, which were obtained for routine diagnostics to assess male infertility were included in this study (no exclusion criteria were applied). Test samples were diluted with 2 mL sterile 0.45% saline, vortexed, and centrifuged