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Semen characterization, seasonality of production, and in vitro sperm quality after chilled storage and cryopreservation in the king penguin (Aptenodytes patagonicus).

Research was conducted to examine seasonal seminal traits and to establish short-term and long-term sperm preservation methods in the king penguin (Aptenodytes patagonicus) for use in genome banking and artificial insemination (AI). Spermic ejaculates (n = 87) obtained using a cooperative method were collected across multiple (n = 6, Male 1) and a single (Male 2) breeding season(s). Non-contaminated ejaculates (n = 69) were 0.36 ± 0.32 ml at 56.3 ± 62.7 × 10(7)  sperm/ml with 85.3 ± 10.6% total motility (TMot), 52.5 ± 12.9% progressive motility (PMot), 86.6 ± 24.3 µm/sec average path velocity

Possibilities of using the European bison (Bison bonasus) epididymal spermatozoa collected post-mortem for cryopreservation and artificial insemination: a pilot study.

BACKGROUND: European bison is the largest mammal in Europe with the population of approximately 4000 individuals. However, there is no report of post-mortem spermatozoa collection and cryopreservation from this species and the aim of this study was to test if the epididymal spermatozoa collected post-mortem from European bison are suitable for cryopreservation and artificial insemination (AI). METHODS: Epididymides were collected post-mortem from two European bison bulls at age of 8 (bull 1) and 11 year (bull 2). Epididymal sperm was harvested by making multiple incisions in caudae epididymid…

Semen coagulum liquefaction, sperm activation and cryopreservation of capuchin monkey (Cebus apella) semen in coconut water solution (CWS) and TES-TRIS.

The objectives of the present study were to test the effect of coconut water solution and TES-TRIS on the seminal coagulum liquefaction, sperm activation in fresh diluted semen, and on the cryopreservation of semen from capuchin monkeys (Cebus apella). Semen was collected from six males by electro-ejaculation, diluted in TES-TRIS or coconut water solution (CWS), and incubated at 35°C until the coagulated fraction of the semen was completely liquefied. In the experiment I, after liquefaction, samples were diluted in TES-TRIS or CWS, plus 6 and 10mM/mL of caffeine. Sperm motility and vigor were …

Recovery, cryopreservation and fertilization potential of bovine spermatozoa obtained from epididymides stored at 5 degrees C by different periods of time.

The objetive of the present study was to evaluate the effect of the interval between animal's death and sperm recovery on the freezability a … storage periods. Conversely, all sperm parameters, except progressive motility, were reduced after cryopreservation. Embryo production was less (P<0.05) in the treatments than in the reference group. However, there was no differences (P>0.05) in blastoycst rate among experimental groups. Considering all the embryos produced by epididymal spermatozoa a greater pro …

The effect of egg yolk, low density lipoproteins, methylxanthines and fertilization diluent on cryopreservation efficiency of northern pike (Esox lucius) spermatozoa.

The effect of egg yolk, low density lipoproteins (LDL) as well as methylxanthines (caffeine and theophylline) and fertilization diluent on cryopreservation efficiency of northern pike, Esox lucius, spermatozoa was tested. Milt was cryopreserved in pellets on dry ice then stored in liquid nitrogen. The extender consisted of 0.6 M sucrose + 15% DMSO supplemented with egg yolk or LDL fractions. The most effective results (77.3% hatched larvae vs 74.1% in the control group) were obtained from extender that contained only 0.6 M sucrose + 15% DMSO and was used for freezing, while the fertilization d…

Immunophenotypic Assessment of Natural Killer Cell Degranulation and Monocyte Function from Cryopreserved Peripheral Blood Mononuclear Cells.

Natural killer (NK) cells and monocytes are central components of the innate immune system, playing critical roles in host defense, immune s … ear cells (PBMCs) are commonly used to enable standardized analyses across time and sites; however, cryopreservation and thawing can adversely affect cell viability and function. This chapter presents a reproducible protocol for the cryopreservation, thawing, and functional assessment of NK cells and monocytes from human PBMCs. PBMCs are cryopreserved using a 9:1 …

Study of the effect of breeding programs in dairy production on cryopreserved ram (Ovis aries) sperm parameters of Manchega sheep.

Breeding programs in dairy sheep (Ovis aries) have significantly increased milk production but have led to a decline in other functional asp … rameters were subtle but detectable. Our results indicate that environmental alterations during the cryopreservation process, specifically the duration of storage, seem to be responsible for the differences between generations, as there were no genetic changes due to selection for the trait "milk production."

Cryopreserved vs Liquid-Stored Platelets for the Treatment of Surgical Bleeding: The CLIP-II Randomized Noninferiority Clinical Trial.

IMPORTANCE: Liquid-stored platelets have a shelf-life of 5 to 7 days, limiting availability and resulting in wastage. OBJECTIVE: To assess the effectiveness and safety of dimethyl sulfoxide-cryopreserved platelets, which have a shelf-life of 2 years, as a treatment for cardiac surgery bleeding. DESIGN, SETTING, AND PARTICIPANTS: The Cryopreserved vs Liquid Platelets II (CLIP-II) trial was a multicenter, randomized, double-blind, parallel-group noninferiority trial, which enrolled patients between August 2021 and April 2024 at 11 Australian tertiary hospitals, with follow-up completed in July

Isolation, Expansion and Characterization of Mesenchymal Stromal Cells from Fresh and Cryopreserved Umbilical Cord Tissues in Horse and Dog.

Mesenchymal stromal cells (MSCs) derived from perinatal tissues represent a promising resource for regenerative medicine due to their proliferative, immunomodulatory, and differentiation properties. Among these tissues, the umbilical cord is considered an accessible and ethically acceptable source of MSCs. This study evaluated the feasibility of whole umbilical cord cryopreservation in canine and equine species and assessed the biological properties of MSCs isolated from cryopreserved tissues compared with fresh samples. Equine Wharton's jelly and canine umbilical cord tissues were processed e…

Cryopreserved vascular allografts for venous lengthening after robot-assisted living donor nephrectomy: a single institution experience.

INTRODUCTION: Living-donor kidney transplantation (LDKT) is the gold standard for end-stage renal disease. Traditionally, the left kidney is preferred for its longer vein. However, the "donor safety first" principle, combined with the transition to laparoscopic and robotic donor nephrectomy, has increased the frequency of using right-sided grafts or encountering "iatrogenically" shortened veins due to mechanical stapling. In this study, we report our preliminary experience evaluating the efficacy of cryopreserved vascular grafts for renal vein lengthening in LDKT to overcome anatomical vascula

Efficacy of Cryopreserved Amniotic Membrane Allograft in the Management of Refractory Chronic Venous Leg Ulcers: A Randomized Controlled Trial.

Background and aims Chronic venous leg ulcers present a clinical challenge due to persistent inflammation, proteolytic imbalance, and senescence of the extracellular matrix. Dehydrated amniotic products are utilized; however, cryopreserved amniotic membrane retains the native heavy chain hyaluronic acid and viable growth factors that are often altered during heat dehydration processing. This study evaluated the efficacy of cryopreserved amniotic membrane allografts in promoting wound closure and reducing pain in patients with refractory venous ulcers compared to standard compression therapy. M

Криопрезентация против пересадки свежих стволовых клеток в автоматической трансплантации для множественной миеломы: воздействие на раннюю токсичность и пересадку.

ВВЕДЕНИЕ: Аутологическая трансплантация стволовых клеток (ASCT) по-прежнему является краеугольным камнем терапии для имеющих на это право пациентов с множественной миеломой. Криопирование гематополиетических трансплантаций обеспечивает логистическую гибкость, но может способствовать повышению токсичности, связанной с процедурой, и использованию ресурсов. Мы провели two- Фаза, неслучайное двухнациональное исследование в высших центрах Сантьяго, Чили, и Севилье, Испания, сравнивая криопрезентированные и некриопретрофированные трансплантаты у пациентов, проходящих ACCT. На ретроспективном этапе20

Совпадение в аннотации оригинала
INTRODUCTION: Autologous hematopoietic stem cell transplantation (ASCT) remains a cornerstone therapy for eligible patients with multiple myeloma. Cryopreservation of hematopoietic grafts provides logistical flexibility but may increase procedure-related toxicity and resource utilization. METHODS: We conducted a two-phase, non-randomized binational study at…

Micro-CT and machine learning: a high-throughput alternative to histology for follicle reserve assessment in cryopreserved ovarian tissue.

BACKGROUND: Ovarian tissue cryopreservation followed by transplantation after cancer remission is a fertility preservation strategy available for certain patient groups, such as pre-pubertal and adolescent girls, as well as adult females requiring urgent gonadotoxic therapy. Quantitative assessment of follicular density in cryopreserved cortical tissue is critical for evaluating tissue quality and estimating its reproductive potential. Conventional analysis, based on manual follicle counts in serial histological sections, is time-consuming, labor-intensive, and prone to variability from uneven…

Application of Cryopreserved Decellularized Human Amniotic Membrane for Healing of Treatment-resistant Cesarean Section Wounds: A Case Series.

Considering the increase in cesarean surgeries in recent years and, therefore, the complications related to this type of surgical wound, attention has been drawn to treatment methods such as biological wound dressings. In this case series, the authors describe the treatment of infected cesarean wounds with impaired healing in 4 women. For this aim, human placentas were collected under sterile conditions and amniotic membranes were separated from the placenta. To decrease immunogenic response, the membrane was decellularized using 1% Triton-x-100, 1 mmol/L EDTA, and Trypsin 0.05. Then, using gl

Punicalagin enhances cryopreserved sheep sperm quality via activation of the Nrf2/PGC-1α pathway and metabolic reprogramming.

Cryopreservation-induced damage remains a major limitation in ram semen preservation, particularly impairing sperm motility and structural integrity. Punicalagin, a natural polyphenol with potent antioxidant properties, has demonstrated potential in mitigating oxidative stress. However, its role in ruminant sperm cryoprotection remains incompletely understood. This study investigated the effects of punicalagin on cryopreserved sheep sperm and explored its underlying molecular mechanisms. A comprehensive evaluation of cryodamage indicators was conducted before and after cryopreservation. The ef…

Comprehensive evaluation of cryopreserved aortic homografts: Long-term mechanical and histological preservation across human and porcine models.

This study aimed to examine the histological and mechanical effects of cryopreservation on human aortic tissues, focussing on storage duration and conditions. Assessments included smooth muscle cell integrity, elastic fibre preservation, and endothelial viability. Cryopreservation with dimethyl sulphoxide (DMSO) significantly reduced smooth muscle cell nuclei loss and maintained elastic fibre integrity. However, elastic fibre thickness increased after 12 months. Isolectin B4 staining showed reduced endothelial cell viability across all groups. No significant changes were observed in mucoid ext…

DNA fragmentation, antioxidant activity and histological structure of cryopreserved testicular tissue depending on sexual maturity and immunological status.

The objective of this work was to determine a relationship between a frequency of DNA fragmentation, a level of antioxidant activity and a preservation of histological structure depending on initial status of fragments of seminiferous tubules of testes (FSTT) of rats at the stages of cryopreservation. FSTT of animals of different ages (immature, mature), as well as animals with changed immunological status (adjuvant arthritis) were cryopreserved. Slow uncontrolled freezing was used in a cryomedium of fibrin gel with 0.7 M glycerol. The results showed that viability, TAS, γGGT and G6PD activiti…

Evaluation of Cryopreserved Ram Sperm with Nano-Ozone Solution and Post-Thaw Life Span by Flow Cytometric Analysis.

Ozone has been used as a therapy tool in medical science for conditions such as ulcers, peritonitis, wounds, and mostly joint problems. Ozone therapy strengthens the resistance to infections by kick-starting antioxidant, anti-inflammatory, and immune modulation systems. Ozone creates a defensive response against oxidative stress in membranes and protects metabolism against reactive oxygen species (ROS). Sperm membranes are one of ROS's main targets; therefore, the cells' cryopreservation process requires more defensive elements for better results. This study aimed to investigate the protective…

Randomized Clinical Trial to Compare Cryopreserved and Lyopreserved Umbilical Cord Tissue to Treat Complex Diabetic Foot Wounds.

To compare the incidence of infection, wound closure and time to wound closure in patients treated with cryopreserved (CPUT) and lyopreserved umbilical tissue (LPUT) in complex diabetic surgical wounds. This single-blinded 12-week randomized clinical trial compared cryopreserved and lyopreserved amniotic cord tissue to treat complex diabetic foot wounds. LPUT or CRAT was applied at baseline and again after four weeks. We enrolled subjects with UT2A-D and 3A-D wounds (depth to tendon, muscle, or bone with infection and/or PAD) and excluded subjects with ABI < 0.5 or TBI < 0.3, untreated osteomy

Establishment of human intestinal organoids derived from commercially available cryopreserved intestinal epithelium and evaluation for pharmacokinetic study.

Human intestinal organoids (HIOs) have been reported to exert their functions in a way that mimics living organs, and HIOs-derived monolayers are expected to be applied to in vitro intestinal pharmacokinetic studies. However, HIOs are established from human tissue, which raises issues of availability and ethics. In the present study, to solve these problems, we have established intestinal organoids using commercially available cryopreserved human intestinal epithelial cells (C-IOs), and compared their functions with biopsy-derived human intestinal organoids (B-IOs) from a pharmacokinetic point