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LC-MS determination and pharmacokinetic studies of ursolic acid in rat plasma after administration of the traditional chinese medicinal preparation Lu-Ying extract.

Sambucus chinensis L. is a native perennial herb distributed throughout China. In traditional Chinese medicine (TCM), this herb is known as Lu-Ying. Ursolic acid is the major effective constituent of Lu-Ying. A rapid, sensitive, and accurate liquid chromatography-mass spectrometry (LC-MS) method for the determination of ursolic acid in rat plasma was developed and validated. Plasma samples taken from rats that had received Lu-Ying extract orally were acidified with acetic acid and then extracted with a mixture of hexane-dichloromethane-2-propanol (20:10:1, v/v/v). Separation of ursolic acid wa…

Medicinal Plants Used Traditionally for Skin Related Problems in the South Balkan and East Mediterranean Region-A Review.

A review research was conducted to provide an overview of the ethnobotanical knowledge of medicinal plants and traditional medical practices … nly used species are Plantago major L. (Albania, Turkey), Hypericum perforatum L. (Greece, Turkey), Sambucus nigra L. (Cyprus, Greece), Ficus carica L. (Cyprus, Turkey), Matricaria chamomilla L. (Cyprus, Greece), and Urtica dioica L. (Albania, Turkey), while many medicinal plants reported by interviewees were common in all four countries. Finally, to relat …

[Effects of ursolic acid on liver-protection and bile secretion].

OBJECTIVE: To study the liver-protection and promoting bile secretion of ursolic acid. METHODS: Ursolic acid was administrated to the mice with liver injury induced by CCl4 or APIT. ALT, AST levels in serum were examined. The bile flow rate and the concentration of the main components in the bile of rats administrated with ursolic acid were estimated. RESULTS: Ursolic acid could decrease the serum ALT, AST, SB levels in the mice treated with CCl4 or APIT, promote the secretion of bile and increase the concentration of bilirubin in the bile. CONCLUSION: Ursolic acid exhibited significant liv

Ethnobotany for food security and ecological transition: wild food plant gathering and consumption among four cultural groups in Kurram District, NW Pakistan.

BACKGROUND: In traditional food systems, especially those of rural populations around the world, wild food plants remain crucial. These reso … luded Fragaria nubicola, Lepidium draba, Pinus wallichiana, Podophyllum emodi, Prunus jacquemontii, Sambucus nigra, Sideroxylon mascatense, and Thymus linearis. Four wild edible mushrooms are also reported for the area for the first time: Calvatia gigantea, Morchella esculenta, Pisolithus albus, and Tulostoma squamosum. The cross-cultural analysis of wild …

Asymmetric effects of native and exotic invasive shrubs on ecology of the West Nile virus vector Culex pipiens (Diptera: Culicidae).

BACKGROUND: Exotic invasive plants alter the structure and function of native ecosystems and may influence the distribution and abundance of arthropod disease vectors by modifying habitat quality. This study investigated how invasive plants alter the ecology of Culex pipiens, an important vector of West Nile virus (WNV) in northeastern and midwestern regions of the United States. METHODS: Field and laboratory experiments were conducted to test the hypothesis that three native leaf species (Rubus allegheniensis, blackberry; Sambucus canadensis, elderberry; and Amelanchier laevis, serviceberry)…

Historical texts as a potential resource for plant-based antiviral agents against SARS-CoV-2: the example of the Receptarium of Burkhard III von Hallwyl from 16th-century Switzerland.

BACKGROUND: In search for effective prophylactic and possibly curative therapeutics against SARS-CoV-2, an historical-ethnopharmacological a … ell viability (> 75%): Artemisia vulgaris L. (aerial parts), Geranium robertianum L. (arial parts), Sambucus nigra L. (leaves) and Viola odorata L. (leaves). The crude extracts were partitioned in aqueous and organic pre-fractions and further analyzed. The ethyl acetate pre-fractions of G. robertianum, S. nigra, and V. odorata expressed significant antivir …

Distribution of sialic acid receptors and experimental infections with different subtypes of influenza A viruses in Qinghai-Tibet plateau wild pika.

BACKGROUND: The plateau pika (Ochotona curzoniae) is a small rabbit-like mammal that lives at high altitudes in the Qinghai-Tibet plateau an … METHODS: The sialic acid receptor distribution tropism in pika was investigated using fluorescent Sambucus nigra and biotinylated Maackia amurensis I and II. Furthermore, the replication of three influenza A viruses H1N1, H3N2, and H5N1 in this animal was examined by immunohistochemistry and RT-PCR. Morphological and histopathological changes caused by i …

Effects of immersion in various beverages, polishing and bleaching systems on surface roughness and microhardness of CAD/CAM restorative materials.

BACKGROUND: The aim of this study was to investigate the effect of an immune-boosting beverage with Sambucus nigra (SAM), an energy beverage (ENE), an in-office bleaching agent with a novel composition, and a fine-grain professional dental prophylaxis (PDP) paste on the surface roughness (Ra) and microhardness (MH) of CAD/CAM restorative materials over time. METHODS: Feldspathic ceramic (FC) and polymer infiltrated ceramic network (PICN) specimens were prepared (N = 126). Glazing, polishing, bleaching and immersion in beverages groups were formed. Bleaching gel containing 25% Hydrogen Peroxid…

Characterization of galactose-containing glycoconjugates in the human retina: a lectin histochemical study.

Seven specimens of morphologically normal formalin-fixed and paraffin-embedded human retina were studied using a panel of fourteen biotinyla … ry epithelium reacted with the same lectins as photoreceptor cells, but it was also labeled by DBA. Sambucus nigra agglutinin (SNA) did not specifically bind to any intraocular structure. These findings favor the theory that, in unpretreated specimens, Gal(beta 1----3)GalNAc (BPA and PNA) is mainly responsible for labeling of cones, while Gal(beta 1----3/4 …

Glycosylation Variability of Serum α1-Acid Glycoprotein in the Context of Developing Inflammation and Oxidative Stress in Patients with Severe COVID-19.

In COVID-19 (coronavirus disease 2019), multi-organ complications depend on the immune system's activity. α1-Acid glycoprotein (AGP) is a hi … and degree of AGP glycosylation were analyzed using lectin-ELISA with lectins: sialo-specific from Sambucus nigra (SNA) and Maackia amurensis (MAA), fucose-specific from Lotus tetragonolobus (LTA) and Aleuria aurantia (AAL). The static and capacitive ORP (sORP and cORP, respectively) were measured using MiOXSYS C+® device (Caerus Biotechnologies, Vilnius, …

Localized reduction of gingival inflammation using site-specific therapy with a topical gingival patch.

METHODS: Sites of inflammation were identified on subjects with moderate-to-severe chronic periodontitis, and were allocated to either patch placement or untreated controls, both for 24 hours. Conventional treatment with scaling and root planing was postponed during the study period. Inflammation was evaluated measuring neutrophilic activity using gingival crevicular fluid (GCF) beta-glucuronidase (b-glu) levels, and clinical response was evaluated using the gingival index (GI). RESULTS: A total of 26 patients were recruited and 36 sites examined, with 22 sites on which the patch was placed a

IgG Glycosylation Profiling of Peripheral Artery Diseases with Lectin Microarray.

BACKGROUND: Inflammation plays a key role in the progression of atherosclerotic plaque for peripheral artery disease (PAD). Immunoglobulin G (IgG) glycosylation could modulate immunological effector functions and has been explored as biomarkers for various diseases. METHODS: Lectin microarray was applied to analyze the expression profile of serum IgG glycosylation in patients with lower-extremity peripheral artery disease (LEPAD), carotid artery stenosis (CAS), abdominal aortic aneurysm (AAA), and healthy controls. Lectin blot was performed to validate the differences. RESULTS: SNA (Sambucus…

Traditional food and herbal uses of wild plants in the ancient South-Slavic diaspora of Mundimitar/Montemitro (Southern Italy).

BACKGROUND: In Europe, only a limited number of cross-cultural comparative field studies or meta-analyses have been focused on the dynamics … cballium elaterium) juice for treating malaria in humans; the aerial parts of the elderberry tree ( Sambucus nigra) for treating erysipelas in pigs; the aerial parts of pellitory ( Parietaria judaica) in decoctions for treating haemorrhoids. CONCLUSIONS: The fact that half of the most salient species documented in our case study - widely available both in …

The hemagglutinin proteins of clades 1 and 2.3.4.4b H5N1 highly pathogenic avian influenza viruses exhibit comparable attachment patterns to avian and mammalian tissues.

UNLABELLED: The global spread of the A/goose/Guangdong/1/96-lineage H5N1 highly pathogenic avian influenza (HPAI) viruses is accompanied by … s where α2,3-linked and α2,6-linked sialyl glycans were detected by Maackia amurensis lectin II and Sambucus Nigra Lectin, respectively. Taken together, the HA proteins of clade 1 and 2.3.4.4b H5N1 viruses generally share comparable attachment patterns to avian and mammalian tissues, despite of evolving into antigenically distinct clades over the past 3 de …

Antiviral potential of medicinal plants against HIV, HSV, influenza, hepatitis, and coxsackievirus: A systematic review.

Viral infections are being managed therapeutically through available antiviral regimens with unsatisfactory clinical outcomes. The refractor … significantly. The current review consolidates the data of the various medicinal plants, those are Sambucus nigra, Caesalpinia pulcherrima, and Hypericum connatum, holding promising specific antiviral activities scientifically proven through studies on experimental animal models. Consequently, the original research addressing the development of novel nutr …

HIV type-1 entry inhibitors with a new mode of action.

BACKGROUND: The development of antiviral drugs has provided crucial new means to mitigate or relieve the debilitating effects of many viral pathogens. Regular use of these drugs has led to generation of resistant strains, making the control of many viral infections very difficult, particularly in HIV-seropositive and AIDS patients. A rich source for the discovery of new HIV infection inhibitors has been, and continues to be, the 'mining' of the large diversity of compounds already available in nature, and specifically those from botanical extracts. METHODS: Using a newly developed direct bind

A novel treatment of gingival recession using a botanical topical gingival patch and mouthrinse.

BACKGROUND AND AIM: Current treatment of gingival recession (GR) is limited to surgical procedures. We describe a case series of 18 patients with GR who were treated with a botanical patch and rinse following standard conservative therapy. CASE SERIES DESCRIPTION: A total of 22 sites with GR > 1 mm were studied. Following scaling and root planing (SRP) and oral hygiene instruction, patients received two courses of patch treatment (3 days each) and botanical rinse administered twice daily throughout the treatment period. Outcome measures (GR, gingival index (GI) and gingival thickness (GT) were

Effect of an herbal mouth rinse in preventing periodontal inflammation in an experimental gingivitis model: a pilot study.

BACKGROUND: Gingivitis is a chronic inflammatory condition, resulting from gingival bacteria and bacterial byproducts. Antiplaque oral rinses reduce inflammation by removing or inhibiting plaque formation. The purpose of this pilot study was to examine the anti-inflammatory effects of HM-302, a mouth rinse based on natural products, on gingival inflammation. METHODS: A prospective, double-blinded, randomized parallel-group controlled trial involving 62 patients was conducted to assess efficacy and safety. During a 2-week period with no dental hygiene, subjects were randomized to receive eithe

Reduced α-2,6 sialylation regulates cell migration in endometriosis.

STUDY QUESTION: Is endometriosis associated with aberrant sialylation patterns and what is the potential impact of such anomalies on cell mi … ients and controls and by western-blot in primary endometrial and endometriotic cell cultures using Sambucus nigra agglutinin (SNA), an α-2,6 sialic acid-binding lectin. A transwell migration assay after incubation of the cells with neuraminidase was also performed to evaluate the impact of desialylation on eutopic endometrial stromal cell migration. MAIN …

Повышенное гликозилирование автоантител во время лечения истощения клеток B.

Для ряда хронических аутоиммунных заболеваний характерна повышенная аутоантибодиозная гликозилация Fab. Остается неясным, связаны ли Fab glycans со состоянием заболевания или развитием, но могут ли они служить его показателем. Многие аутоиммунные заболевания лечатся с помощью методов лечения истощения клеток В, что в первую очередь приводит к сокращению числа автоантител. Возникает вопрос, может ли терапия разрушения клеток B оказать воздействие на гликозилацию Fab. Здесь мы изучили продольное воздействие терапии истощения клеток B на гликозилацию Fab для общего ИгГ и IgG автоантифитов в ревма

Совпадение в полном тексте источника
… Lectin affinity chromatography Sialylated proteins were purified from patient samples by Sambucus nigra agglutinin (SNA) affinity chromatography as described previously 40 . …