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Estimating unbiased haplotype frequencies from stem cell donor samples typed at heterogeneous resolutions: a practical study based on over 1 million German donors.

The human leukocyte antigen (HLA) distribution in donor registry data is typically nonrandom as, mostly for economical reasons, typing additional loci or resolving ambiguities is selectively performed based on the previously known HLA type. Analyzing a sample of over 1 million German stem cell donors, we practically show the extent of the bias caused by the restriction of the input data for HLA haplotype frequency (HF) estimation to subsets selected according to their higher HLA typing resolution and, conversely, the correctness of estimates based on unselected data with a methodology suitable

Secrets and lies and donor conceptions: what donor-conceived individuals feel about their disclosure/discovery experience.

STUDY QUESTION: What are the satisfaction levels, as defined by emotional comfort or contentment, of adult individuals conceived via donor-assisted reproduction concerning the method, timing, and circumstances surrounding the disclosure/discovery of their conception? SUMMARY ANSWER: Inadvertent discovery and older age at the time of disclosure of donor-conceived status are associated with lower rates of satisfaction among donor-conceived individuals. WHAT IS KNOWN ALREADY: The proliferation of commercial DNA testing has resulted in many donor-conceived people learning inadvertently of their

Recruitment of aged donor heart with pharmacological stress echo. A case report.

BACKGROUND: The heart transplant is a treatment of the heart failure, which is not responding to medications, and its efficiency is already proved: unfortunately, organ donation is a limiting step of this life-saving procedure. To counteract heart donor shortage, we should screen aged potential donor hearts for initial cardiomyopathy and functionally significant coronary artery disease. Donors with a history of cardiac disease are generally excluded. Coronary angiography is recommended for most male donors older than 45 years and female donors older than 50 years to evaluate coronary artery st

A Narrative Review of Anterior Cruciate Ligament Reconstruction Graft Choice and Its Influence on Return to Sport.

PURPOSE OF REVIEW: Anterior cruciate ligament (ACL) rupture is among the most common knee injuries in active individuals and can lead to persistent instability, secondary intra-articular damage, and an elevated risk of osteoarthritis. Because return to sport (RTS) is a major goal for most active patients and graft selection remains one of the most debated aspects of ACL reconstruction, this narrative review summarizes the contemporary evidence on how graft choice influences RTS, focusing on the four most common grafts: bone-patellar tendon-bone (BTB), hamstring tendon (HT), quadriceps tendon (

Outcomes of germ line testing for children with hematologic malignancies undergoing hematopoietic cell transplantation.

Recent studies reveal that a growing proportion of children with hematologic malignancies (HM) harbor germ line pathogenic or likely pathoge … to ensure timely counseling and germ line testing to detect a hereditary predisposition and inform donor selection for HCT.

A 2026 Update in DSEK Graft Delivery Techniques and Devices.

PURPOSE: Corneal transplantation has evolved from penetrating keratoplasty to selective component keratoplasty, with endothelial keratoplasty (EK) becoming the preferred treatment for most endothelial disorders. EK offers faster visual rehabilitation, reduced immunologic rejection, and longer graft survival. This review intends to describe various Descemet stripping endothelial keratoplasty (DSEK) graft insertion techniques and devices, clinical outcomes, and surgical ease, to facilitate evidence-based technique selection. METHODS: This review critically appraises graft insertion techniques a

Новальная граница в исследовании оксиса Гут-Брэйна: трансплантация фекалий микробиота в неевродегенеративных расстройствах.

Ось кишечника (GBA) представляет собой сложную двунаправленную систему связи, соединяющую центральную нервную систему (CNS) и желудочно-кишечный тракт (GI). Такое взаимодействие происходит главным образом через нейронные, иммунные и метаболические пути. Дисбиоз в кишечной микробиоте связан с многочисленными нейродегенеративными заболеваниями, такими, как болезнь Паркинсона (PD), болезнь Альцгеймера (AD), множественный склероз (MS) и амиотропический боковой склероз (ALS). В последние годы в качестве новаторского терапевтического подхода к трансплантации фекальной микробиоты (ФМТ) была привлечен

Совпадение в аннотации оригинала
The gut-brain axis (GBA) represents a sophisticated bidirectional communication system connecting the central nervous system (CNS) and the g … ry and neuromuscular functions in ALS. Despite these findings, several challenges remain, including donor selection complexities, uncertainties about long-term safety, and inconsistencies in clinical outcomes. Innovations such as synthetic microbial communities, engineered probiotics, and AI-driven analysis of the microbiome hold the potential to improve the prec …

Differential immunogenicity of paternal HLA Class I antigens in pregnant women.

More insight into the differential immunogenicity of human leukocyte antigen (HLA) mismatches will be beneficial for donor selection in clinical transplantation. In this study the immunogenicity of HLA antigens was analyzed by examining the antibody profiles in women who have been pregnant. In total 888 women, who had pregnancy induced HLA alloantibodies, were included in this study, while 413 women who had not been immunized by their pregnancy, served as controls. First it was analyzed whether women expressing particular HLA antigens are more likely to produce HLA alloantibodies. Next we dete…

Acute Alcohol-Associated Hepatitis: The Role of Liver Transplantation.

Severe alcohol-associated hepatitis (AH) remains one of the most challenging syndromes in hepatology and critical care, with high short-term mortality despite advances in supportive management. Although corticosteroids remain the only guideline-endorsed pharmacologic therapy shown to improve early survival, their benefit is modest, limited to selected patients, and frequently complicated by infection. Consequently, contemporary management extends well beyond corticosteroid therapy and requires meticulous supportive care, nutritional optimization, infection surveillance, management of portal hy

Predicting graft thickness in ultrathin DSAEK using a mechanical microkeratome system.

PURPOSE: To examine pre-cut factors associated with post-cut graft thickness in ultrathin Descemet stripping automated endothelial keratoplasty (UT-DSAEK) prepared with a mechanical microkeratome system and to develop a nomogram to support blade selection. METHODS: Donor corneas processed at a single eye bank were included. All tissues were dissected using a standardised mechanical microkeratome protocol with fixed pressure and cutting speed. Pre-cut variables included central pachymetry, microkeratome blade size, endothelial cell density, cause of death and demographic characteristics. Multi

Applying Intraoperative Portal Venography in Liver Transplantation Vascular Surgery.

Background/Aim: Adequate portal inflow is essential for liver graft regeneration following transplantation. Intraoperative portal venography (IOPV) provides real-time assessment of portal vein patency, stenosis, thrombus formation, and portosystemic collaterals. In addition to imaging, portal vein pressure gradient (portal vein pressure minus inferior vena cava pressure) was also measured. This study assessed the impact of IOPV on surgical decision-making and post-transplant outcomes to establish criteria for patient selection. Methods: From November 2016 to November 2024, 34 liver transplant

Management of deep sacral and ischial pressure injuries with free-style local perforator flaps: A D+P+DPD model.

BACKGROUND: Previous reports on the treatment of sacral and ischial pressure injuries have not provided clear algorithms for surgical therapies. The objective of this study was to establish a reconstruction algorithm to guide the selection of an ideal free-style perforator flap that can be tailored to the defect in question. METHODS: We used 23 perforator flaps to reconstruct 14 sacral and 8 ischial defects in 22 patients over 5 years. A reconstruction algorithm system was developed based on the anatomical features of the perforator vessels (diameter, D; pulsatility [++∼+++], P) and their pos

Liver Transplantation for Peri-hilar Cholangiocarcinoma.

BACKGROUND: Liver transplantation for peri-hilar cholangiocarcinoma (pCCA) following neoadjuvant chemoradiation achieves excellent long-term survival in carefully selected patients with early-stage unresectable pCCA and patients with primary sclerosing cholangitis (PSC)-associated pCCA. Strict adherence to selection criteria, aggressive neoadjuvant therapy, operative staging prior to transplantation, and several technical accommodations during the transplant operation are necessary for success. In this review, we provide a contemporaneous overview of liver transplantation for pCCA, including s

Formula versus donor breast milk for feeding preterm or low birth weight infants.

BACKGROUND: When sufficient maternal breast milk is not available, alternative sources of enteral nutrition for preterm or low birth weight infants are donor breast milk or artificial formula. Donor breast milk may retain some of the non-nutritive benefits of maternal breast milk for preterm or low birth weight infants. However, feeding with artificial formula may ensure more consistent delivery of optimal levels of nutrients. Uncertainty exists about the balance of risks and benefits of feeding formula versus donor breast milk for preterm or low birth weight infants. OBJECTIVES: To determine

Interaction of bromine vapor with nitrogen-based electron-donor molecules: thermodynamic, kinetic, and DFT investigations.

CONTEXT: Molecular bromine (Br₂) is an important halogen in atmospheric chemistry and is also associated with environmental contamination. Nitrogen-containing donor molecules in methanolic solutions interact selectively with Br₂, leading to the formation of donor-bromine complexes. The diffusion and reactivity of bromine vapor were investigated using UV-visible spectroscopy in the 200-600 nm range. Bromine concentrations and reaction products were quantified through calibration curves and mathematical modeling. Photophysical and thermodynamic parameters, including molar extinction coefficients

Survival and rehabilitation after orthotopic liver transplantation.

Between May, 1968, and April 27, 1980, 94 patients have been treated by orthotopic liver transplantation. During this time operative techniques and the management and selection of patients have changed. Healing of the biliary-tract anastomoses has been better since the introduction of a gallbladder conduit procedure and early irrigation of the donor biliary tract largely prevents damage to biliary-tract mucosa and sludge formation. Partial cardiopulmonary bypass in selected cases can provide control of the circulation during surgery. Many patients have been operated on too late, and an earlier

Sleep Anxiety and Influencing Factors in Patients After Kidney Transplant: A Latent Profile Analysis.

OBJECTIVES: Our objective was to investigate the average Kidney Donor Profile Index in the Serbian kidney donor population and its influence on kidney transplant function. MATERIALS AND METHODS: We conducted a noninterventional retrospective study that included brain dead donors with data between 2010 and 2016. We analyzed the following donor data: sex, age, body mass, body height, race, hypertension and diabetes mellitus, cause of death, terminal creatinine level, hepatitis C positivity, existence of circulatory death before donation, and calculated Kidney Donor Profile Index. We analyzed th

Utilization of Hepatitis C Virus-Infected Donor Hearts in Two Children and Two Young Adults: Initial Experience at a Pediatric Transplant Center.

BACKGROUND: Although adult transplant centers are successfully transplanting organs from hepatitis C virus (HCV)-infected donors with detectable viral load by nucleic acid testing (NAT+) into HCV-negative recipients, this practice has not yet been adopted widely by the pediatric heart transplant community. METHODS: We present a case series of four patients who received heart transplants from HCV NAT+ donors at a pediatric transplant center, including two pediatric patients < 18 years of age. RESULTS: All recipients tolerated a 12-week course of glecaprevir/pibrentasvir and achieved a sustain

Patient and Kidney Allograft Survival with National Kidney Paired Donation.

BACKGROUND AND OBJECTIVES: In the United States, kidney paired donation networks have facilitated an increasing proportion of kidney transplants annually, but transplant outcome differences beyond 5 years between paired donation and other living donor kidney transplant recipients have not been well described. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: Using registry-linked data, we compared National Kidney Registry (n=2363) recipients to control kidney transplant recipients (n=54,497) (February 2008 to December 2017). We estimated the risk of death-censored graft failure and mortality usi

Donor-to-recipient weight ratio is a risk factor for hepatic artery thrombosis after whole-liver transplantation in children under 25 kg.

Hepatic artery thrombosis (HAT) following pediatric liver transplantation increases morbidity and risk of graft failure. We performed a retrospective chart review of all patients who underwent deceased-donor liver transplantation from August 2002 to July 2016. Multi-organ transplant recipients were excluded. We examined the incidence of HAT at our institution and sought to identify associated donor or recipient risk factors. A total of 127 deceased-donor liver transplant patients with a median age of 1.7 years (IQR 0.67-6.7) were identified. Of those, 14 developed HAT, all weighing under 25 kg