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Хуангки Гуизхи Вуву декокассирует экспериментальный аутоиммунный энцефаломиэлит, пропагандируя INK1/Parkin-посредованно митофагию и подавляя микроглиальный пироптоз.

Множественный склероз (MS) — хроническое аутоиммунное расстройство центральной нервной системы. Хуангки Гуйцхи Уву Декокац (HGWD), классическая традиционная китайская медицина, оказывает защитное воздействие на MS, однако основополагающий механизм, связанный с митофагией и микроглиальным пироптозом, остается недостаточно изученным. В ходе этого исследования изучалось положительное воздействие ГГВД на экспериментальный аутоиммунный энцефаломиэлит (EAE) и его молекулярный механизм. С помощью UHPLC-Q-Orbitrap HRMS были выявлены компоненты HGWD, и потенциальные цели против MS были предсказаны с по

Совпадение в аннотации оригинала
Multiple sclerosis (MS) is a chronic autoimmune disorder of the central nervous system. Huangqi Guizhi Wuwu Decoction (HGWD), a classic trad … rly elucidated. This study investigated the ameliorative effects of HGWD on experimental autoimmune encephalomyelitis (EAE) and its molecular mechanism. HGWD components were identified via UHPLC-Q-Orbitrap HRMS, and potential anti-MS targets were predicted by network pharmacology and molecular docking. EAE mice and LPS-stimulated microglia were treated with HGWD, w …

Managing Energy, and Shaping Care: Insights from Adults with Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Through Co-Production Workshops.

BACKGROUND: Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a complex, debilitating condition characterized by severe fatigue that is not relieved by rest and is often exacerbated by physical or mental activity. A key challenge for individuals with ME/CFS is energy management and to date, the only recommended strategy is "activity pacing." This approach involves balancing activity and rest to avoid overexertion and minimize the risk of symptom exacerbation, commonly known as "post-exertional malaise". A recent systematic review highlighted significant shortcomings in activity pa…

Improving myalgic encephalomyelitis population sampling: Applying an online respondent-driven method to address biases in G93.3 register data.

With widespread late- and under-diagnosing, health register code G93.3 data cannot offer an unbiased sampling frame for myalgic encephalomyelitis, complicating prevalence and demographic distribution assessments. It also remains unclear if all G93.3 cases would meet the Canada Consensus Criteria (CCC). This article describes a novel methodological approach to addressing selection bias when estimating a CCC population's characteristics, applying an online respondent-driven sampling approach and validated DePaul University algorithms. In a sample of 660 respondents, we assess possible bias in th…

Gender and Age Differences in Seronegative Pediatric Acute Disseminated Encephalomyelitis Profiles: Results and Insights from a Tertiary Center.

OBJECTIVES: Acute disseminated encephalomyelitis (ADEM) is a rapid-onset inflammatory central nervous system (CNS) disorder in children, causing demyelination, encephalopathy, and neurological deficits, often following infections. MATERIALS & METHODS: This 10-year retrospective study evaluated pediatric patients with seronegative acute disseminated encephalomyelitis (ADEM), focusing on clinical, laboratory, and imaging profiles. The various profiles were assessed to determine age- and/or sex-based differences. RESULTS: The study reviewed 36 patients, with an average age of 6.08 years and pre…

Unravelling the Connection Between Energy Metabolism and Immune Senescence/Exhaustion in Patients with Myalgic Encephalomyelitis/Chronic Fatigue Syndrome.

Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a debilitating disease, characterized by a diverse array of symptoms including post-exertional malaise (PEM), severe fatigue, and cognitive impairments, all of which drastically diminish the patients' quality of life. Despite its impact, no curative treatments exist, largely due to the limited understanding of the disease's underlying pathophysiology. Mitochondrial dysfunction, leading to impaired energy production and utilization, is believed to play a key role in the onset of fatigue and PEM, positioning it as a potential key pat…

Временное развитие аксоновой дегенерации в визуальной системе в экспериментальной аутоиммунной энцефаломиэлите: Отчёты от нейропатологии с высоким разрешением.

Множественный склероз (MS) характеризуется воспалением, обезвоживанием и аксоновой дегенерацией в CNS, что приводит к постепенной неврологической инвалидности, как правило, рассматривается как аутоиммунное расстройство. Визуальное расстройство, частый симптом, является результатом повреждения клеток сетчатки (КЖК) и их аксонов на переднем визуальном пути. Используя экспериментальную модель аутоиммунного энцефаломиелита (EAE) в мышах, мы использовали несколько методов, включая нейропатологию с высоким разрешением с использованием новой иммуногистохимической технологии на сечениях ультратина, дл

Совпадение в аннотации оригинала
Multiple sclerosis (MS) is characterized by inflammation, demyelination, and axonal degeneration in the CNS, leading to progressive neurolog … lion cells (RGCs) and their axons in the anterior visual pathway. Using the experimental autoimmune encephalomyelitis (EAE) model in mice, we used several methods, including high-resolution neuropathology with a novel immunohistochemical technique on ultrathin sections, to characterize axonal pathology and demyelination, terminal disruption, perikaryal degeneration …

CRTH2 имеет решающее значение для клеток IL-1β, производящих B, во время экспериментального аутоиммунного энцефаломиелита у мышей с помощью сигнала p38.

Клетки B играют решающую роль в патогенезе аутоиммунных подстрекательских заболеваний, таких, как множественный склероз (МС). Как рецептор простагландина D2, молекула гомологового рецептора, выраженная на клетках TH2 (CRTH2), как известно, участвует в активации клеток TH2, но ее функция в B лимфоцитах не ясна. Здесь мы показываем, что CRTH2 является критическим для подмножества клеток, производящих IL-1β. Удаление мышей из клеток B-специализированного типа Crth2 свидетельствует о сокращении количества клеток IL-1β-производящих B, что приводит к улучшению экспериментального аутоиммунного энцефа

Совпадение в аннотации оригинала
B cells play a critical role in the pathogenesis of autoimmune inflammatory diseases such as multiple sclerosis (MS). As a receptor of prost … it reduced numbers of IL-1β-producing B cells, resulting in amelioration of experimental autoimmune encephalomyelitis (EAE), the principal animal model of MS. Compared to wild-type B cells, adoptive transfer of Crth2-deficient B cells attenuates EAE disease severity in B-cell-deficient recipient mice. The IL-1β-producing B cell subpopulation was mainly transitional …

Нейропохранительная роль аллопрегнанолона в смягчении депрессивного поведения путем модуляции функции мозга в экспериментальной аутоиммунной модели энцефаломиелита.

ГОЛОСОВАНИЕ: Депрессия относится к числу наиболее распространенных коморбидов, связанных с MS, что существенно снижает качество жизни пациентов и снижает прогноз. В этом исследовании мы изучили терапевтические последствия аллопрегнанолона (АП) для депрессивного поведения в крысиной модели MS. МЕТОДЫ: Для создания крысиной модели экспериментального аутоиммунного энцефаломиелита (EAE) использовались женские крысы Льюиса (6-8 недель, 160-180 г). Тесты на поведение дали оценку депрессивному поведению. Внутренняя активность мозга оценивалась с помощью функционального магнитного резонансного изображ

Совпадение в аннотации оригинала
BACKGROUND: Depression is among the most common comorbidities associated with MS, significantly impairing patients' quality of life and prog … ewis rats (6-8 weeks old, 160-180g) were used to establish the rat model of experimental autoimmune encephalomyelitis (EAE). The behavioral tests evaluated depression-like behaviors. The intrinsic activity in the brain were assessed using functional magnetic resonance imaging (fMRI). For pathological and molecular expression detection, hematoxylin-eosin (HE) and Lu …

Оксиматрин регулирует микроглию для производства IFN-β путем активации траектории STING/TBK1/IRF3 против экспериментального аутоиммунного энцефаломиелита.

Оксиматрин — алкалоид с иммуномодуляцией. Недавние исследования показали, что оксиматрин препятствует экспериментальному аутоиммунальному энцефаломиелиту (EAE) — животной модели множественного склероза (MS), поощряя производство интерферона-β (IFN-β). Однако механизм, с помощью которого оксиматрин регулирует производство ИФН-β, остается неясным. Цель этого исследования заключалась в изучении фармакологических последствий и связанных с ними молекулярных механизмов оксиматина при лечении EAE в ходе экспериментов в vivo и in vitro. Оксиматрин смягчил неврологическую дисфункцию, обезвоживание и во

Совпадение в аннотации оригинала
Oxymatrine is an alkaloid with the property of immunomodulation. Recent studies have demonstrated that oxymatrine inhibits experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS), by promoting the production of interferon-β (IFN-β). However, the mechanism through which oxymatrine regulates the production of IFN-β remains u…

Визуальное участие предшествует механической дисфункции в экспериментальном аутоиммунном энцефаломиэлите: свидетельства неинвазивной электрофизиологии и изображений.

Экспериментальная аутоиммунная энцефаломелит (EAE) модель множественного склероза связана с аномалиями в визуальном (VEP) и моторном (MEP) потенциале, а также нейроретинным разрежением в оптической томографии (OCT). Время возникновения этих дефицитов имеет решающее значение для опробования новых терапевтических стратегий. Так, VEP, MEP и OCT были выполнены на продромальных, острых и хронических фазах EAE. Группа мышей EAE приносилась в жертву для гистологического анализа в каждый момент времени. Задержка с запозданием на продромальных фазах была связана с ранним опустыниванием зрительного нерв

Совпадение в аннотации оригинала
The Experimental Autoimmune Encephalomyelitis (EAE) model of Multiple Sclerosis is associated with abnormalities in visual (VEP) and motor (MEP) evoked potentials, together with neuroretinal thinning at optical coherence tomography (OCT). The time course of these deficits is pivotal for testing novel therapeutic strategies. Thus, VEP, MEP, and OCT were perfo…

Graded exercise therapy compared to activity management for paediatric chronic fatigue syndrome/myalgic encephalomyelitis: pragmatic randomized controlled trial.

The MAGENTA pragmatic parallel groups randomized controlled trial compared graded exercise therapy (GET) with activity management (AM) in treating paediatric myalgic encephalomyelitis or chronic fatigue syndrome (ME/CFS). Children aged 8-17 years with mild/moderate ME/CFS and presenting to NHS specialist paediatric services were allocated at random to either individualised flexible treatment focussing on physical activity (GET, 123 participants) or on managing cognitive, school and social activity (AM, 118 participants) delivered by NHS therapists. The primary outcome was the self-reported sho…

Dry eye symptoms and signs in United States Gulf War era veterans with myalgic encephalomyelitis/chronic fatigue syndrome.

BACKGROUND: To examine ocular symptoms and signs of veterans with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) diagnosis, ME/CFS symptoms, and controls. METHODS: This was a prospective, cross-sectional study of 124 South Florida veterans in active duty during the Gulf War era. Participants were recruited at an ophthalmology clinic at the Miami Veterans Affairs Hospital and evaluated for a diagnosis of ME/CFS, or symptoms of ME/CFS (intermediate fatigue, IF) using the Canadian Consensus criteria. Ocular symptoms were assessed via standardised questionnaires and signs via compreh…

Developing a Blood Cell-Based Diagnostic Test for Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Using Peripheral Blood Mononuclear Cells.

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is characterized by debilitating fatigue that profoundly impacts patients' lives. Diagnosis of ME/CFS remains challenging, with most patients relying on self-report, questionnaires, and subjective measures to receive a diagnosis, and many never receiving a clear diagnosis at all. In this study, a single-cell Raman platform and artificial intelligence are utilized to analyze blood cells from 98 human subjects, including 61 ME/CFS patients of varying disease severity and 37 healthy and disease controls. These results demonstrate that Ra…

Does physical exercise improve or deteriorate treatment of multiple sclerosis with mitoxantrone? Experimental autoimmune encephalomyelitis study in rats.

BACKGROUND: Mitoxantrone has proved efficacy in treatment of multiple sclerosis (MS). The fact that physical exercise could slow down the progression of disease and improve performance is still a debatable issue, hence; we aimed at studying whether combining mitoxantrone with exercise is of value in the management of MS. METHODS: Thirty-six male rats were divided into sedentary and exercised groups. During a 14-day habituation period rats were subjected to exercise training on a rotarod (30 min/day) before Experimental Autoimmune Encephalomyelitis (EAE) induction and thereafter for 17 consecu…

A Spontaneous Model of Experimental Autoimmune Encephalomyelitis Provides Evidence of MOG-Specific B Cell Recruitment and Clonal Expansion.

The key role of B cells in the pathophysiology of multiple sclerosis (MS) is supported by the presence of oligoclonal bands in the cerebrospinal fluid, by the association of meningeal ectopic B cell follicles with demyelination, axonal loss and reduction of astrocytes, as well as by the high efficacy of B lymphocyte depletion in controlling inflammatory parameters of MS. Here, we use a spontaneous model of experimental autoimmune encephalomyelitis (EAE) to study the clonality of the B cell response targeting myelin oligodendrocyte glycoprotein (MOG). In particular, 94% of SJL/j mice expressing…

Multimodal MRI of myalgic encephalomyelitis/chronic fatigue syndrome: A cross-sectional neuroimaging study toward its neuropathophysiology and diagnosis.

INTRODUCTION: Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), is a debilitating illness affecting up to 24 million people worldwide but concerningly there is no known mechanism for ME/CFS and no objective test for diagnosis. A series of our neuroimaging findings in ME/CFS, including functional MRI (fMRI) signal characteristics and structural changes in brain regions particularly sensitive to hypoxia, has informed the hypothesis that abnormal neurovascular coupling (NVC) may be the neurobiological origin of ME/CFS. NVC is a critical process for normal brain function, in which gluta…

Clinical effects of wasabi extract containing 6-MSITC on myalgic encephalomyelitis/chronic fatigue syndrome: an open-label trial.

BACKGROUND: Wasabi (Eutrema japonicum) is a common pungent spice used in Japan. 6-Methylsulfinylhexyl isothiocyanate (6-MSITC) found in the rhizome of wasabi has been shown to have anti-inflammatory and antioxidant effects, as well as improve neuroinflammation and memory. Therefore, we hypothesized that these effects would be beneficial for treating myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). The present study was conducted to investigate the effectiveness of wasabi extract containing 6-MSITC on ME/CFS in an open-label trial. METHODS: Fifteen patients (3 males, 12 females, 20…

Naringenin attenuates experimental autoimmune encephalomyelitis by protecting the intact of blood-brain barrier and controlling inflammatory cell migration.

Targeting pathogenic immune cell trafficking poses an attractive opportunity to attenuate autoimmune disorders such as multiple sclerosis (MS). MS and its animal model, experimental autoimmune encephalomyelitis (EAE), are characterized by the immune cells-mediated demyelination and neurodegeneration of the central nervous system (CNS). Our previous study has proven that dietary naringenin ameliorates EAE clinical symptoms via reducing the CNS cell infiltration. The present study examined the beneficial effects of naringenin on maintaining the blood-brain barrier in EAE mice via dietary naringe…

Reductions in Cerebral Blood Flow Can Be Provoked by Sitting in Severe Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Patients.

INTRODUCTION: In a large study with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) patients, we showed that 86% had symptoms of orthostatic intolerance in daily life and that 90% had an abnormal reduction in cerebral blood flow (CBF) during a standard tilt test. A standard head-up tilt test might not be tolerated by the most severely affected bed-ridden ME/CFS patients. Sitting upright is a milder orthostatic stress. The present study examined whether a sitting test, measuring cerebral blood flow by extracranial Doppler, would be sufficient to provoke abnormal reductions in cerebr…