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Exploiting the message from cancer: the diagnostic value of extracellular vesicles for clinical applications.

Liquid biopsy is indispensable for the resolution of current medical issues, such as the cost of developing new drugs and predicting responses of patients to drugs. In this sense, not only the technology for liquid biopsy but also the target biomolecules for biomarkers need to be identified. Extracellular vesicles (EVs), which contain various proteins, including membrane-bound proteins, and RNAs, including mRNA and long/short noncoding RNAs, have emerged as ideal targets for liquid biopsy. These complex biomolecules are covered by a lipid bilayer, which can protect them from degradation. In th

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… Pavlou MP Diamandis EP The cancer cell secretome: a good source for disco- vering biomarkers? J. Proteom. 2010 73 1896 1906 10.1016/j.jprot.2010.04.003 20394844 3. …

Hematopoietic Niche Hijacking in Bone Metastases: Roles of Megakaryocytes, Erythroid Lineage Cells, and Perivascular Stromal Subsets.

Bone metastases mark a critical and often terminal phase in cancer progression, where disseminated tumor cells (DTCs) manage to infiltrate and exploit the complex microenvironments of the bone marrow. While most current therapies focus on the well-known late-stage "vicious cycle" of osteolysis, they often overlook the earlier stages, namely, tumor cell colonization and dormancy. During these early phases, cancer cells co-opt hematopoietic stem cell (HSC) niches, using them as sanctuaries for long-term survival. In this review, we bring together emerging insights that highlight a trio of undera

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… Beyond SCF, endothelial ANGPTL2 sustains HSC function, emphasizing a broader pro-hematopoietic secretome from the vascular wall [ 23 ]. Megakaryocytes co-localize with sinusoids and restrain the HSC pool in mice [ 19 , 26 , 27 ]. MKs act as negative regulators of HSC function [ 19 ]. … Proteomic and secretome profiling indicate that megakaryocytes play a role in stromal reprogramming; they store TGF-β1, PDGF, and IGF1 in α-granules and release these mediators upon activation [ 120 ]. …

Adding recombinant AAVs to the cancer therapeutics mix.

Gene therapy is a powerful biological tool that is reshaping therapeutic landscapes for several diseases. Researchers are using both non-viral and viral-based gene therapy methods with success in the lab and the clinic. In the cancer biology field, gene therapies are expanding treatment options and the possibility of favorable outcomes for patients. While cellular immunotherapies and oncolytic virotherapies have paved the way in cancer treatments based on genetic engineering, recombinant adeno-associated virus (rAAV), a viral-based module, is also emerging as a potential cancer therapeutic thr

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… The cells of a tumor that metastasize to a secondary organ express a genetic profile that is different from the primary tumor, a characteristic of tumor heterogeneity that in some ways makes metastatic cancer its own distinct disease. 130 , 132 , 134 , 136 For example, several groups report specific secretome … Global secretome analysis identifies novel mediators of bone metastasis. Cell Res. 2012;22:1339–1355. doi: 10.1038/cr.2012.89. 140. Yao Y., Fang Z.P., Chen H., Yue L., Min D.L., Tang L.N., Yu W.X., Kung H.F., Lin M.C., Shen Z. …

Skin Telocytes Could Fundament the Cellular Mechanisms of Wound Healing in Platelet-Rich Plasma Administration.

For more than 40 years, autologous platelet concentrates have been used in clinical medicine. Since the first formula used, namely platelet-rich plasma (PRP), other platelet concentrates have been experimented with, including platelet-rich fibrin and concentrated growth factor. Platelet concentrates have three standard characteristics: they act as scaffolds, they serve as a source of growth factors and cytokines, and they contain live cells. PRP has become extensively used in regenerative medicine for the successful treatment of a variety of clinical (non-)dermatological conditions like alopec

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… TCs distinctiveness resides in their particular (ultra)structural features, their microRNA expression, and their proteomics and secretomics [ 10 ]. At the skin level, TCs are found in both the papillary and reticular dermis. This has been evidenced using various techniques. … However, it will be interesting to document the interaction between pharmaceutical agents like non-steroidal anti-inflammatory drugs (NSAIDs) and PRP administration, in terms of the potential alteration of the PRP secretome induced by NSAIDs. …

Natural killer cells, macrophages and dendritic cells as innate immune therapies for blood cancers.

Innate immune cells are emerging as powerful allies in the fight against blood cancers. Natural Killer (NK) cells, macrophages, and dendritic cells (DCs)—once viewed mainly as supporting players—are now at the forefront of next-generation immunotherapies, owing to their rapid, antigen-independent tumor recognition and potent effector functions. This review highlights their distinct anti-tumor roles: NK cells’ cytotoxicity and “missing-self” recognition, the dual and adaptable nature of macrophages in the tumor microenvironment (TME), and the antigen-presenting capabilities of DCs that bridge i

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… Cho S Choi SH Maeng E Park H Ryu KS Park KS Boosting tumor homing of endogenous natural killer cells via therapeutic secretomes of chemically primed natural killer cells J Immunother Cancer 2025 13 3 010371 10.1136/jitc-2024-010371 PMC11883546 40044578 Cho S, Choi SH, Maeng E, Park H, Ryu KS, Park KS … (2025) Boosting tumor homing of endogenous natural killer cells via therapeutic secretomes of chemically primed natural killer cells. …

Novel cellular immunotherapies for hematological malignancies: recent updates from the 2021 ASH annual meeting.

Cellular immunotherapy, including the chimeric antigen receptor T (CAR-T) cell therapy and CAR- natural killer (CAR-NK) cell therapy, has undergone extensive clinical investigation and development in recent years. CAR-T cell therapy is now emerging as a powerful cancer therapy with enormous potential, demonstrating impressive anti-tumor activity in the treatment of hematological malignancies. At the 2021 ASH annual meeting, numerous breakthroughs were reported concerning acute lymphocytic leukemia (ALL), lymphoma, acute myeloid leukemia (AML), and multiple myeloma (MM). Universal CAR-T cell an

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… Caroline Diorio et al. performed comprehensive secretome profiling to measure more than 1400 serum analytes in serial samples collected from patients treated by CD19 CAR-T cells [ 107 ]. Two novel pre-infusion biomarkers, MILR1 and FLT3, were identified to predict the development of CRS. … Comprehensive secretome profiling elucidates novel disease biology and identifies pre-infusion candidate biomarkers to predict the development of severe cytokine release syndrome in pediatric patients receiving CART19. Blood. 2021;138(Supplement 1):167. doi: 10.1182/blood-2021-149477. 108. …

Cell Therapy: Types, Regulation, and Clinical Benefits.

Cell therapy practices date back to the 19th century and continue to expand on investigational and investment grounds. Cell therapy includes stem cell- and non-stem cell-based, unicellular and multicellular therapies, with different immunophenotypic profiles, isolation techniques, mechanisms of action, and regulatory levels. Following the steps of their predecessor cell therapies that have become established or commercialized, investigational and premarket approval-exempt cell therapies continue to provide patients with promising therapeutic benefits in different disease areas. In this review

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… In regenerative medicine, ASCs are considered the most promising among cell therapies, and ADSCs constitute an ideal option due to their ease of harvest requiring minimal invasiveness; multi-lineage differentiation potential; and anti-inflammatory and proangiogenic secretome ( 255 ). … These benefits are linked to the immunomodulatory actions of MSCs originating mostly from their immune inhibitory secretome ( 82 ). …

Mechanisms, Diagnosis and Treatment of Bone Metastases.

Bone and bone marrow are among the most frequent metastatic sites of cancer. The occurrence of bone metastasis is frequently associated with a dismal disease outcome. The prevention and therapy of bone metastases is a priority in the treatment of cancer patients. However, current therapeutic options for patients with bone metastatic disease are limited in efficacy and associated with increased morbidity. Therefore, most current therapies are mainly palliative in nature. A better understanding of the underlying molecular pathways of the bone metastatic process is warranted to develop novel, wel

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… In turn, the secretome of bone marrow MSCs was shown to promote the expression of the 47 kDa CXCR4 isoform and also increased MMP-9 secretion, expression of integrin α3 and integrin β1, supporting the invasive potential of neuroblastoma cells [ 199 ]. … The hypoxic cancer secretome induces pre-metastatic bone lesions through lysyl oxidase. Nature. 2015;522:106–110. doi: 10.1038/nature14492. 69. Zoni E., van der Pluijm G. The role of microRNAs in bone metastasis. J. Bone Oncol. 2016;5:104–108. doi: 10.1016/j.jbo.2016.04.002. 70. …

Influence of the Mediterranean Diet on Healthy Aging.

The life expectancy of the global population has increased. Aging is a natural physiological process that poses major challenges in an increasingly long-lived and frail population. Several molecular mechanisms are involved in aging. Likewise, the gut microbiota, which is influenced by environmental factors such as diet, plays a crucial role in the modulation of these mechanisms. The Mediterranean diet, as well as the components present in it, offer some proof of this. Achieving healthy aging should be focused on the promotion of healthy lifestyle habits that reduce the development of pathologi

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… AD is included in the group of SASP or pathologies secondary to an altered secretome (growth factors, ROS, cytokines, and metalloproteinases). In fact, late astrocytes generate increased secretion of SASP factors that deregulate physiological functions. … Initially, secretome factors could prevent tumor progression or eradicate malignant cells except in situations where there is acute aggression to SASP [ 323 ]. …

MSC-EV therapy for bone/cartilage diseases.

Mesenchymal stromal cells (MSCs) have been utilized in cell therapy for various diseases. Recent studies have demonstrated that extracellular vesicles (EVs) released by MSCs play an important role in their therapeutic activities. EVs contain a variety of bioactive molecules such as proteins, messenger RNAs (mRNAs), and micro RNAs (miRNAs) and modify the function of the recipient cells by transferring these molecules. Despite the promising potential of EV therapy as a substitute for MSC therapy, there are challenges that need to be addressed for the clinical success of EV therapy. EV quality ha

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… similarities among the immunophenotypes and differentiation abilities of these MSCs from different sources; however, further characterization revealed differences in their gene expression profiles ( Arjmand et al., 2019 ; Elahi et al., 2016 ; Roson-Burgo et al., 2014 ; Secco et al., 2009 ) and proteome/secretome … Considerable variability has been found in the secretome profiles of MSCs isolated from different donors in response to inflammatory stimuli ( Zhukareva et al., 2010 ). …

Oral Presentations

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Malignant Pleural Effusion: Palliative Managements and Indication for Pleurodesis Based on Survival Scores.

Neoplastic pleural effusion is one in which malignant neoplastic cells are detected in the pleural fluid or in the parietal pleura. When secondary, it confirms disseminated neoplastic disease and suggests a reduced life expectancy and low quality of life. This review was described by a group of physician members of the Brazilian Society of Oncological Surgery regarding the treatment of neoplastic pleural effusion, developed to guide surgeons, palliative care physicians and clinical oncologists in their clinical practice to assess patients indicated for pleurodesis based on survival scores.

Протеомический анализ показывает дисрегламентацию периферийных нейтрофилов крови у пациентов с множественным склерозом.

Множественный склероз (MS) представляет собой сложное автовоспалительное заболевание мозга и спинного мозга, которое приводит к аксоновому демилеляции и симптомам, включая усталость, боль и трудности с зрением и мобильностью. Вовлеченность иммунной системы в патологию MS хорошо известна, в частности адаптивная реакция T-клеток, и особое внимание уделяется производящему IL-17 поднабору клеток TH17 и их роли в вождении болезней. Однако важность врожденных иммунных клеток не была столь хорошо охарактеризована. Здесь мы сосредоточили внимание на нейтрофилах, которые являются врожденными иммунными

Navigating uncharted waters: assessing the impact of the COVID-19 pandemic on hematopoietic stem cell transplantation: challenges and innovations.

The COVID-19 pandemic has significantly impacted hematopoietic stem cell transplantation (HSCT), necessitating adaptations across pre-transplant, transplantation, and post-transplant phases. HSCT recipients with compromised immune systems face heightened risks of severe COVID-19 outcomes, including increased mortality. The pandemic prompted significant changes in treatment strategies, with many patients experiencing delays or deferrals in autologous stem cell transplantation (ASCT), alongside adjustments to chemotherapy regimens to prevent disease recurrence. Clinical practices have evolved to

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… Mesenchymal stromal cells (MSCs) play a crucial role in these processes, as their secretome can drive inflammaging when the cells become senescent. …

Bibliometric analysis of research trends in the relationship between frailty and neoplasms over the past decade.

BackgroundThe relationship between frailty and neoplasms has attracted increasing attention from researchers in recent years. This study aims to identify current research hotspots and status in this field through bibliometric and visualization analysis.MethodsLiterature on the relationship between frailty and neoplasms, meeting the inclusion criteria, was collected from the Core Collection. Bibliometric analysis and visualization were performed using WoS, VOSviewer, and CiteSpace.ResultsOur study included 7410 documents on frailty and neoplasms, authored by 43,605 researchers from 9478 institu

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… Justice JN, Leng XI, LeBrasseur NK, Tchkonia T, Kirkland JL, Mitin N, Liu Y, Kritchevsky SB, Nicklas BJ, Ding J (2024) Caloric restriction intervention alters specific circulating biomarkers of the senescence-associated secretome in middle-aged and older adults with obesity and prediabetes in an 18-week …

Melanoma bone metastasis-induced osteocyte ferroptosis via the HIF1α-HMOX1 axis.

Osteocytes are the main cells in mineralized bone tissue. Elevated osteocyte apoptosis has been observed in lytic bone lesions of patients with multiple myeloma. However, their precise contribution to bone metastasis remains unclear. Here, we investigated the pathogenic mechanisms driving melanoma-induced osteocyte death. Both in vivo models and in vitro assays were combined with untargeted RNA sequencing approaches to explore the pathways governing melanoma-induced osteocyte death. We could show that ferroptosis is the primary mechanism behind osteocyte death in the context of melanoma bone m

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… Fig. 2 B16F10 Secretome-Induced Osteocyte Ferroptosis. a PCA plot showing sample patterns of individual samples in the control and B16F10-CM treated MLO-Y4 cells. b KEGG pathway analysis indicating “Ferroptosis” as the significantly altered pathway. c GO pathway analysis of B16F10-CM treated vs. untreated … Venn diagram of intersecting genes between bone-related and MLO-Y4 cell-related ferroptosis genes Fig. 2 B16F10 Secretome-Induced Osteocyte Ferroptosis. a PCA plot showing sample patterns of individual samples in the control and B16F10-CM treated MLO-Y4 cells. b KEGG pathway analysis indicating “Ferroptosis …

The mutual impacts of stem cells and sleep: opportunities for improved stem cell therapy.

Sleep is an indispensable physiological function regulated by circadian rhythms, which influence the biological pathways and overall health of the body. Sleep is crucial for the maintenance and restoration of bodily systems, and disturbances can lead to various sleep disorders, which can impair both mental and physical health. Treatment options for these disorders encompass lifestyle modifications, psychotherapy, medications, and therapies such as light therapy and surgery. Not only sleep deprivation has a significant impact on essential organs, but it also influences various types of stem cel

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… In vivo Synchronizes intestinal cells; promotes stem cell circadian clock function Modulation of Wnt, Hippo, and Notch pathways [ 79 ] MSCs Recurrent airway obstruction Rat; serum from rat In vivo Induces early MSC mobilization into bloodstream; enhances MSC regenerative potential Anti-inflammatory secretome … Synchronizes intestinal cells; promotes stem cell circadian clock function | Modulation of Wnt, Hippo, and Notch pathways | [ 79 ] MSCs | Recurrent airway obstruction | Rat; serum from rat | In vivo | Induces early MSC mobilization into bloodstream; enhances MSC regenerative potential | Anti-inflammatory secretome …

The Role of Exercise in Cancer-Related Sarcopenia and Sarcopenic Obesity.

One of the most common adverse effects of cancer and its therapeutic strategies is sarcopenia, a condition which is characterised by excess muscle wasting and muscle strength loss due to the disrupted muscle homeostasis. Moreover, cancer-related sarcopenia may be combined with the increased deposition of fat mass, a syndrome called cancer-associated sarcopenic obesity. Both clinical conditions have significant clinical importance and can predict disease progression and survival. A growing body of evidence supports the claim that physical exercise is a safe and effective complementary therapy f

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… These muscle-secreted factors that constitute the muscle secretome, act in a paracrine, autocrine, or endocrine manner and mediate, directly or indirectly, some of the beneficial effects of exercise on cancer patients’ clinical outcomes. …

Cellular Senescence and the Kidney: Potential Therapeutic Targets and Tools.

Chronic kidney disease (CKD) is an increasing health burden (affecting approximately 13.4% of the population). Currently, no curative treatment options are available and treatment is focused on limiting the disease progression. The accumulation of senescent cells has been implicated in the development of kidney fibrosis by limiting tissue rejuvenation and through the secretion of pro-fibrotic and pro-inflammatory mediators termed as the senescence-associated secretory phenotype. The clearance of senescent cells in aging models results in improved kidney function, which shows promise for the op

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… In contrast, senescent cells stop expressing proliferation markers (e.g., Ki-67) and produce a senescence-associated secretome (SASP; see SASP in CKD). Another particularly interesting feature of senescent cells is the loss of the structural nuclear lamina component lamin B1. … An impaired immune system (due to aging, disease, or immunosuppressive therapy) may cause some senescent cells to evade elimination and maintain an SASP secretome ( Lujambio, 2016 ). …

Extracellular vesicles: a new frontier in diagnosing and treating graft-versus-host disease after allogeneic hematopoietic cell transplantation.

Graft-versus-host disease (GvHD) is a prevalent complication following allogeneic hematopoietic stem cell transplantation (HSCT) and is characterized by relatively high morbidity and mortality rates. GvHD can result in extensive systemic damage in patients following allogeneic HSCT (allo-HSCT), with the skin, gastrointestinal tract, and liver frequently being the primary target organs affected. The severe manifestations of acute intestinal GvHD often indicate a poor prognosis for patients after allo-HSCT. Endoscopy and histopathological evaluation remain employed to diagnose GvHD, and auxiliar

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… mesenchymal stem cells for patients with chronic graft versus host disease | Unknown status; Phase 2/3 | cGvHD | Bone marrow injection of hMSCs | NCT01526850 25 | MSC for the treatment of cGVHD after allo-HSCT | Unknown status; Phase 2 | cGvHD | Infusion of BMMSCs | NCT04692376 26 | Effect of PMSCs and their secretome … for the treatment of GvHD | Completed; Phase 3 | GvHD | Infusion /skin spray of hPMSCs and their secretome | NCT06469411 27 | Mesenchymal stem cells for the treatment of refractory chronic graft versus host disease | Unknown status; Phase 2 | cGvHD | Infusion of BMMSCs | NCT01765660 28 | Treatment of …