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Comparative immunochemical and biological analysis of African and South American yellow fever viruses.

Four geographic variants (topotypes) of yellow fever (YF) virus from Africa and South America previously determined by RNA oligonucleotide mapping were analyzed for their structural, antigenic and virulence characteristics. The electrophoretic migration mobility and carbohydrate content of the envelope protein E characterized YF virus strains of South America. The NS 1 protein of South American and Central African YF virus strains was not precipitated by anti-NS 1 monoclonal antibodies (MAB) that precipitated NS 1 of West African strains. No distinction could be made among the YF virus strains…

Nucleotide sequence of yellow fever virus: implications for flavivirus gene expression and evolution.

The sequence of the entire RNA genome of the type flavivirus, yellow fever virus, has been obtained. Inspection of this sequence reveals a single long open reading frame of 10,233 nucleotides, which could encode a polypeptide of 3411 amino acids. The structural proteins are found within the amino-terminal 780 residues of this polyprotein; the remainder of the open reading frame consists of nonstructural viral polypeptides. This genome organization implies that mature viral proteins are produced by posttranslational cleavage of a polyprotein precursor and has implications for flavivirus RNA rep…

Human antibody response to immunization with 17D yellow fever and inactivated TBE vaccine.

The antibody response against flaviviruses tick-borne encephalitis (TBE), Kyasanur Forest disease (KFD), Murray Valley encephalitis (MVE), West Nile fever (WNF), Japanese B encephalitis (JE), dengue 2 (DEN-2), and yellow fever (YF) was studied in humans after administration of an inactivated TBE virus vaccine. Individuals were either prevaccinated with 17D yellow fever (experimental group) or without any previous exposure to flaviviruses (control group). The appearance of serum titres of homologous and heterologous haemagglutination inhibition (HI) antibodies, heterotypic DEN-2 neutralizing an…

Yellow fever outbreak in South Eastern State of Nigeria--virological and serological studies.

An outbreak of Yellow Fever in South Eastern State involving Uyo, Itu and Abak divisions is presented. The occurrence of few jaundiced cases with fever in places 29-35 km outside Uyo indicated that the epidemic was more widely spread than it was thought. The rural communities appeared to be more severely affected where a considerably large number of unrecorded deaths were reported. Mortality rate was about 56% among hospitalised patients. The diagnosis of Yellow Fever was confirmed by virus isolation and serology This was the first recorded outbreak in this area.

Yellow fever vaccine: direct challenge of monkeys given graded doses of 17D vaccine.

In rhesus monkeys a wide dosage range of 17D yellow fever (YF) vaccine extending to a level even below that recommended for vaccination of man elicited an immune response providing solid protection to challenge with virulent YF virus. Forty-three of 45 monkeys vaccinated with 10(2.3) or greater weanling mouse mean lethal doses of 17D vaccine were resistant to challenge 20 weeks later with virulent Asibi strain YF virus. Monkeys given graded doses of lesser amounts of vaccine were progressively more susceptible to challenge. With a vaccine dose >/= 10(2.3) weanling mouse mean lethal doses, plaq…

Международный совместный анализ международной справочной подготовки антижелтоголовой сыворотки.

В 1962 году Комитет экспертов ВОЗ по биологической стандартизации учредил сыворотку желтой лихорадки под названием " Международная справочная подготовка антижелезнокожих сывороток " , предназначенную для использования в качестве сыворотки иммунной системы при испытании на защиту мышей, используемом при контроле за вакцинами от желтой лихорадки. Результаты испытаний на нейтрализацию были последовательными, а относительная эффективность была достаточно высокой. В настоящем документе не только содержится информация о совместном анализе, но и обсуждаются ошибки в методах испытания и проб

Совпадение в аннотации оригинала
A yellow fever immune serum was established in 1962 by the WHO Expert Committee on Biological Standardization as the International Reference Preparation of Anti-Yellow-Fever Serum, intended to serve as an immune reference serum in the mouse protection test used in the control of yellow fever vaccines.Before its establishment as the International Reference Pr…

ETIOLOGY OF YELLOW FEVER : XI. SERUM TREATMENT OF ANIMALS INFECTED WITH LEPTOSPIRA ICTEROIDES.

The use ot a polyvalent immune serum ot nign potency in tne treatment of an experimental infection of guinea pigs with Leptospira icteroides … n. In man the clinical manifestations are more gradual and distinct than in the guinea pig, yet the yellow fever patient whose temperature is sub-normal, and who has reached the stage of hemorrhages from the gums, nose, stomach, and intestines, and of uremia and cholemia, would seem to have little or no chance of deriving benefit from the use of a specific imm …

ETIOLOGY OF YELLOW FEVER : III. SYMPTOMATOLOGY AND PATHOLOGICAL FINDINGS IN ANIMALS EXPERIMENTALLY INFECTED.

Studies are reported on the type of disease induced in guinea pigs, dogs, and monkeys by inoculating them (1) with the blood or organ emulsi … erved in animals experimentally infected with Leptospira icteroides closely parallel those of human yellow fever. The pathological changes occurring in human cases of yellow fever are similar to those induced by inoculation in guinea pigs and marmosets and in respect to their intensity stand intermediate between those arising in the two animals mentioned.