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Isolation of Chlamydia trachomatis from prostatic fluid in association with inflammatory joint or eye disease.

We describe two patients, one with peripheral arthritis, sacro-iliitis, positive HLA B27, and autoantibodies to smooth muscle and gastric parietal cell; the other with aphthoid ulcers, geographical tongue, conjunctivitis, anterior uveitis, peripheral arthralgia, and diarrhoea with distal proctocolitis. Neither patient would have been diagnosed as having urethritis on the basis of accepted microscopic criteria. In both patients, however, Chlamydia trachomatis was isolated from the prostatic fluid, but not the urethra.

Host response to primary Chlamydia trachomatis infection of the fallopian tube in pig-tailed monkeys.

Experimental acute salpingitis was produced in four pig-tailed monkeys, Macaca nemestrina, by intratubal inoculation with Chlamydia trachomatis (serotypes E or F). The organisms were reisolated from both the endosalpinx and endocervix as early as 1 week after the original inoculation. Endosalpinx cellular responses to the infection were examined by light, transmission, and scanning electron microscopy. A moderate lymphocyte infiltration was detected in the submucosa on day 7. By days 14 and 21 the lymphocytic infiltration was heavy and extended into both the submucosa and the mucosa; the infil…

Correlation of host immune response with quantitative recovery of Chlamydia trachomatis from the human endocervix.

We studied 95 women with uncomplicated Chlamydia trachomatis cervical infection. Quantitative isolation of C. trachomatis was performed in HeLa 229 cells, and the results were correlated with serum immunoglobulin M and immunoglobulin G antibody to the organism. We found that quantitative cultures for C. trachomatis can provide a meaningful measurement by which to evaluate the effect of the acquired immune response. In particular, secretory immunoglobulin A antibody to C. trachomatis in cervical secretion demonstrated a striking and inverse correlation with recovery of the organism from the cer…

Homologues of the Chlamydia trachomatis and Chlamydia muridarum Inclusion Membrane Protein IncS Are Interchangeable for Early Development but Not for Inclusion Stability in the Late Developmental Cycle.

Chlamydia trachomatis is an obligate intracellular bacterium, which undergoes a biphasic developmental cycle inside a vacuole termed the inclusion. Chlamydia-specific effector proteins embedded into the inclusion membrane, the Inc proteins, facilitate inclusion interaction with cellular organelles. A subset of Inc proteins engages with specific host factors at the endoplasmic reticulum (ER)-inclusion membrane contact site (MCS), which is a discrete point of contact between the inclusion membrane and the endoplasmic reticulum (ER). Here, we report that the C. trachomatis Inc protein CTL0402/Inc…

In vitro inhibitory effect of Hydrocotyle bonariensis Lam. extracts over Chlamydia trachomatis and Chlamydia pneumoniae on different stages of the chlamydial life cycle.

Chlamydial infections in humans are widely distributed and are responsible for a variety of acute and chronic diseases. Both Chlamydia trachomatis and Chlamydia pneumoniae can lead to chronic conditions that have been linked to complications and sequelae. This study aimed to develop a culture method in order to detect in vitro antichlamydial activity of different extracts obtained from native Argentinian plants used as antimicrobials in local ethnomedicine and to evaluate their inhibitory activity over Chlamydia trachomatis and Chlamydia pneumoniae growth. The inhibitory activity over differen…

Antibodies to Variable Domain 4 Linear Epitopes of the Chlamydia trachomatis Major Outer Membrane Protein Are Not Associated with Chlamydia Resolution or Reinfection in Women.

Chlamydia trachomatis is an obligate intracellular bacterium. C. trachomatis infection is the most prevalent bacterial sexually transmitted infection and can lead to pelvic inflammatory disease and infertility in women. There is no licensed vaccine for C. trachomatis prevention, in part due to gaps in our knowledge of C. trachomatis-specific immune responses elicited during human infections. Previous investigations of the antibody response to C. trachomatis have identified immunodominant antigens and antibodies that can neutralize infection in cell culture. However, epitope-specific responses …

Evaluation of Cortez OneStep Chlamydia Rapicard™ Insta Test for the Detection of Chlamydia trachomatis in Pregnant Women at Mbare Polyclinic in Harare, Zimbabwe.

BACKGROUND: Cervical chlamydia infection poses high risk of pregnancy complications and neonatal infection. Reference methods for the detection of chlamydia infection are not available for routine use in developing countries. Point-of-care (POC) tests can bridge this gap. This study evaluated Cortez Onestep Chlamydia Rapicard™ insta test for the detection of Chlamydia trachomatis in pregnant women at Mbare Polyclinic and determined the prevalence of C. trachomatis. METHODS: This was a cross sectional study in 242 pregnant women aged ≥18 years attending their first ANC visit at Mbare polyclini…

Wnt/Notch Crosstalk Promotes Epithelial-Mesenchymal Transition During Chlamydia trachomatis Infection Under IFN-γ Treatment.

Chlamydia trachomatis (Ct) infection is a major cause of infertility, primarily through inducing tubal fibrosis. Epithelial-mesenchymal transition (EMT) plays a central role in this fibrotic process. However, the molecular mechanisms by which Ct infection triggers EMT are not fully understood. IFN-γ is a major component of the host immune pressure acting on infected epithelial cells during Ct infection. To mimic this immune environment in vitro and investigate how host signaling responses to Ct infection influence EMT, we maintained Ct-infected HeLa cells under continuous IFN-γ treatment and e…

The Chlamydia trachomatis-secreted effector protein CT181 binds to Mcl-1 and prolongs neutrophil survival.

Chlamydia trachomatis (C.t.) infections can lead to severe complications due to the pathogen's ability to evade the host immune response, often resulting in asymptomatic infections. The mechanisms underlying this immune subversion remain incompletely understood, but likely involve specific bacterial effector proteins. Here, we identify CT181 as a novel effector that binds to Mcl-1, a key regulator of neutrophil survival. While a C.t. CT181 mutant exhibited only modest defects in epithelial cell replication and inclusion development, it was required for C.t. survival in neutrophils, which corre…

Chlamydia trachomatis genovariant L2b in men who have sex with men in Taiwan, 2020-2023.

OBJECTIVES: Lymphogranuloma venereum (LGV) caused by Chlamydia trachomatis genotypes L1-L3 has been resurging among men who have sex with men (MSM) and people with HIV (PWH) in Western countries. While historically attributed to tropical regions, rectal LGV has been rarely recognised in Asia, with Taiwan recently becoming the second Asian country to report cases. METHODS: A multicentre, laboratory-based surveillance was conducted from January 2020 to December 2023 in Taiwan. Specimens were collected from MSM through syndromic testing and screening of high-risk populations. C. trachomatis was …

The second messenger signaling molecule cyclic di-AMP drives developmental cycle progression in Chlamydia trachomatis.

The obligate intracellular bacterium Chlamydia alternates between two functional forms during its developmental cycle: elementary body (EB) and reticulate body (RB). However, the molecular mechanisms governing the transitions between these forms are unknown. Here, we present evidence cyclic di-AMP (c-di-AMP) is a key factor in triggering the transition from RB to EB (i.e., secondary differentiation) in the chlamydial developmental cycle. By overexpressing or knocking down expression of c-di-AMP synthase genes, we made strains producing different levels of c-di-AMP, which we linked to changes i

По микробным характеристикам цервиковагинальной железы хламидиотрахоматис распространяется на верхние гениталии инфицированных женщин.

ВВЕДЕНИЕ: Инфекция хламидии трахоматиса (КТ) может привести к воспалительным заболеваниям таза, бесплодию и другим репродуктивным последствиям, когда она поднимается на верхний гениальный тракт. Факторы, включая хламидиальное бремя, совместное заражение с другими бактериальными патогенами, передаваемыми половым путем, и использование оральных противозачаточных средств влияют на риск распространения верхних гениталий. Цервиковагинальный состав микробиома влияет на подверженность КТ, и мы исследовали, способствует ли он распространению путем анализа вариантов ампликонной последовательности (ASV)

Совпадение в аннотации оригинала
INTRODUCTION: Chlamydia trachomatis (CT) infection can lead to pelvic inflammatory disease, infertility and other reproductive sequelae when it ascends to the upper genital tract. Factors including chlamydial burden, co-infection with other sexually-transmitted bacterial pathogens and oral contraceptive use influence risk for upper genital tract spread. Cerv…

Sexually transmitted infections (Chlamydia trachomatis, genital HSV, and HPV) and female fertility: A scoping review.

More than one million sexually transmitted infections (STIs) are acquired every day worldwide. They are commonly caused by Chlamydia trachomatis (CT), herpes simplex virus (HSV), or human papillomavirus (HPV) and are suggested to contribute to female infertility. This scoping review aims to map and summarize existing literature on the association between a pre-pregnancy infection with CT, genital HSV, or HPV and female fertility. We searched two databases (PubMed, Embase) to identify published literature on the association between CT, genital HSV, or HPV infection and female fertility. We incl…

Prevalence of Ocular Chlamydia trachomatis and Active Trachoma among Children in Merhabete District, Amhara, Ethiopia.

There have been significant reductions in the burden of trachoma worldwide. However, some districts have experienced persistently high trach … (based on clinical assessment) and ocular chlamydia (based on polymerase chain reaction to identify Chlamydia trachomatis). A total of 5,935 children were examined in 80 communities. The prevalence of trachomatous inflammation-follicular (TF) was 46.6%, trachomatous inflammation-intense (TI) was 17.5%, and ocular chlamydia was 28.0%. The correlation between TF and ocul …

Chlamydia trachomatis TmeA promotes pedestal-like structure formation through N-WASP and TOCA-1 interactions.

UNLABELLED: Chlamydia trachomatis (C.t.) is the causative agent of several human diseases, including the sexually transmitted infection chlamydia and the eye infection trachoma. As an obligate intracellular bacterial pathogen, invasion is critical for establishing infection and subsequent pathogenesis. During invasion, C.t. secretes effector proteins via its type III secretion system (T3SS), which manipulate host actin cytoskeletal regulation to promote bacterial entry. Previous studies identified the T3SS effector protein TmeA as a key factor in C.t. invasion, as it recruits and activates N-W…

Unique T cell signatures associated with reduced Chlamydia trachomatis reinfection in a highly exposed cohort.

Chlamydia trachomatis (CT) is the most common bacterial sexually transmitted infection globally. Understanding natural immunity to CT will inform vaccine design. This study aimed to profile immune cells and associated functional features in CT-infected women, and determine immune profiles associated with reduced risk of ascended endometrial CT infection and CT reinfection. PBMCs from CT-exposed women were profiled by mass cytometry and random forest models identified key features that distinguish outcomes. CT+ participants exhibited higher frequencies of CD4+ Th2, Th17, and Th17 DN CD4 T effec…

HLA-DR and HLA-DQ Polymorphism Correlation with Sexually Transmitted Infection Caused by Chlamydia trachomatis.

Background and Objectives: Chlamydia trachomatis (C. trachomatis) represents one of the most prevalent bacterial sexually transmitted diseases. This study aims to explore the relationship between HLA alleles/genotypes/haplotypes and C. trachomatis infection to better understand high-risk individuals and potential complications. Materials and Methods: This prospective study recruited participants from Transylvania, Romania. Patients with positive NAAT tests for C. trachomatis from cervical/urethral secretion or urine were compared with controls regarding HLA-DR and -DQ alleles. DNA extraction f…