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Maxillary sinus augmentation with autologous bone harvested from the lateral maxillary wall combined with bovine-derived hydroxyapatite: clinical and histologic observations.

OBJECTIVE: The aim of this study was to determine the clinical efficacy of a mixture of autologous bone harvested from the lateral wall of t … t, leading to a survival rate of 98.9%. The new bone consisted of lamellae of living bone contained osteocytes and in close contact with bovine bone particles that were partly infiltrated by newly formed bone. Bovine bone particle resorption could not be found. The histomorphometric analysis showed the following averages: 29% of newly formed bone and 21% of …

Patients with primary hyperparathyroidism have lower circulating sclerostin levels than euparathyroid controls.

OBJECTIVE: In vitro and in vivo studies in animal models have shown that parathyroid hormone (PTH) inhibits the expression of the SOST gene, which encodes sclerostin, an osteocyte-derived negative regulator of bone formation. We tested the hypothesis that chronic PTH excess decreases circulating sclerostin in humans. DESIGN: We studied 25 patients with elevated serum PTH concentrations due to primary hyperparathyroidism (PHPT) and 49 patients cured from PHPT after successful parathyroidectomy (PTx; euparathyroid controls (EuPTH)). METHODS: We measured plasma PTH and serum sclerostin levels a

Influence of hormones on osteogenic differentiation processes of mesenchymal stem cells.

Bone development, regeneration and maintenance are governed by osteogenic differentiation processes from mesenchymal stem cells through to m … he development of osteogenic offspring from mesenchymal stem cells, which end up in osteoblasts and osteocytes. Hormones are involved in fetal bone development and regeneration and, in childhood, adolescence and adulthood, they control adaptive needs for growth and reproduction, nutrition, physical power and crisis adaptation. As in other tissues, aging in m …

[Radiologic and histologic observations in central talus fractures].

In a prospective study of central fractures of the talus, radiological data (plain radiography, CT, MRI) were scrutinized in terms of correlation with histological findings (biopsy taken at time of screw removal). The study looked at nine fractures in eight patients aged 14-48 years. There was one fracture of Hawkins type I, one fracture of type II, four fractures of type III, and three corpus fractures. All were operated on within 24 h after injury; seven had screw fixation, two had adaptation with K wires. The patients were kept non-weight-bearing for 14-53 weeks. Duration of follow-up was 2

Experimental studies on osteoporosis.

Nutritional secondary hyperparathyroidism (NSH) defines a spontaneous and experimental disease in most domesticated and in some wild animals, caused by dietary calcium deficiency and/or phosphorus excess. Calcium deficiency results directly in hypocalcemia, and phosphorus excess induces hyperphosphatemia which causes hypocalcemia. Secondary hyperparathyroidism thus results and the plasma parameters return to normal and are maintained but only at the expense of progressive bone loss. The bone loss is generalized but the bones are not uniformly affected. The hierarchy of bone loss is, in decreas

The effect of Emdogain gel on periodontal regeneration in autogenous transplanted dog's teeth.

AIM: The aim of this study was to assess the enamel matrix derivative (EMD) as biomaterial used in autogenous tooth transplantation. MATERIAL AND METHODS: The study consisted of 3 beagle dogs, 5 months old, in which six incisors and six premolars were transplanted to mechanically prepared recipient alveolar sockets. One group of teeth was transplanted to a recipient bed, with EMD in the root surface. The second groups of teeth were transplanted using saline solution in the root surface. Every week, clinical examinations were done. Nine weeks later, the animals were killed and the specimens de

[Stem cells in the amniotic fluid: the new chance of regenerative medicine].

Amniotic fluid has been used in prenatal diagnosis for more than decades. It yields a simple and reliable screening and diagnostic tool for a variety of congenital malformations and genetic diseases such as chromosomal aberrations, neural tube defects or storage diseases. Nowadays the widening knowledge provides evidence that amniotic fluid is not only a screening and diagnostic tool, but it may be also the source of the effective therapy of several congenital and adult disorders. A subset of cells, the so-called stem cells were found in the amniotic fluid as well as the placenta, and they pro

Osteogenic and adipogenic differentiation potential of an immortalized fibroblast-like cell line derived from porcine peripheral blood.

Primary fibroblast-like cells isolated from the peripheral blood of a healthy pig were immortalized by transduction of cells with a replication-defective retrovirus vector expressing the E6/E7 proteins of human papillomavirus type 16 (pLXSN-16E6E7). The immortalized cells grow rapidly in cell culture and exhibit a distinct cell surface phenotype that was positive for CD90, CD44, collagen I, and vimentin and negative for CD14 and MHC II. Additionally, these immortalized blood derived-fibroblast-like cells had the potential to differentiate into osteoblasts and adipocytes in vitro as evidenced b

In vitro heterogeneity of osteogenic cell populations at various equine skeletal sites.

Bone cell cultures were evaluated to determine if osteogenic cell populations at different skeletal sites in the horse are heterogeneous. Osteogenic cells were isolated from cortical and cancellous bone in vitro by an explant culture method. Subcultured cells were induced to differentiate into bone-forming osteoblasts. The osteoblast phenotype was confirmed by immunohistochemical testing for osteocalcin and substantiated by positive staining of cells for alkaline phosphatase and the matrix materials collagen and glycosaminoglycans. Bone nodules were stained by the von Kossa method and counted.

Tideglusib-Preactivated Osteoblasts Encapsulated in Ceramic Particle-Reinforced GelMA Hydrogel for Enhanced Critical-Size Bone Defect Regeneration.

PURPOSE: Critical-size bone defects remain a clinical challenge due to limited intrinsic bone regeneration. This study developed a composite … .N, Tb.Th, and BMD in the GPCT group versus others. Histology showed more mature bone with numerous osteocytes, partial degradation of BCP particles, and new bone area fraction >70% in GPCT. CONCLUSION: The composite BCP particle/GelMA scaffold with Tideglusib-pretreated OBs provides mechanical reinforcement and sustained osteogenic stimulation, effectively …

The Crucial Role of Exercise in Promoting Adolescent Bone Health.

This chapter comprehensively summarizes the pivotal role of exercise in fostering optimal bone health during adolescence, a critical period … ing, remodeling, and ossification-are elucidated, detailing the coordinated actions of osteoblasts, osteocytes, and osteoclasts. The interplay of these processes in shaping bone size, density, and strength in response to mechanical forces and hormonal fluctuations during puberty is thoroughly discussed. This chapter further incorporates findings from key stu …

Intracellular Ca2+ waves in mammalian cells.

Intracellular calcium waves refer to the coordinated propagation of increased free calcium ion (Ca2+) concentration in the cytoplasm. Ca2+ i … arized cells with processes, but without excitability: the radial glia, astrocytes, Müller glia and osteocytes as a cell does not connect strongly to sensory-neural structures.

Development of In Vitro Parkinson's Disease Model Mediated by MPP+ and α-Synuclein Using Wharton's Jelly Mesenchymal Stem Cells.

MAIN PROBLEM: The mechanism behind Parkinson's disease (PD) is still unclear, and a cure to stop its progression is yet to be found. This is … SCs were positive for MSC markers and were able to differentiate into adipocytes, chondrocytes, and osteocytes. DAN obtained after the differentiation of WJMSCs for 48 h expressed neuronal markers such as synapsin 1, neuropilin, neurofilament, and MAPT along with dopaminergic markers such as Nurr1, DAT, TH, DDC, and KCNJ6 and were functionally active. Upon d …

Antiresorptive effect of a semisynthetic triterpene from Combretum leprosum Mart. on dexamethasone-induced osteoporosis in rats.

Chronic use of glucocorticoids is one of the most common causes of osteoporosis. Triterpenes have a positive effect on bone metabolism, whic … n in L2. In the analysis of the femurs, CL-P2 promoted an increase in the number of osteoblasts and osteocytes and a reduction in the number of osteoclasts, as well as change in the mineral composition of these bones, suggested by the increase in the carbonate-to-phosphate ratio (CTPR) identified by micro-Raman spectrometry. Histopathological analysis of the …

Оценка взаимодействия между седловатыми стержневыми клетками Уортона и β-Меркаптоэтанолом.

ЦЕЛЬ: Железные мезенхимальные стволовые клетки Уортона (WJ-MSC) являются саморегенеративными и способными дифференцировать в многопоточные стволовые клетки. Могут существовать различные источники мезенхимальных стволовых клеток (МСЦ), задействованных в механизме ремонта поврежденных тканей в организме. WJ-MSC могут проводить различие между остеоцитами, хондроцитами, адипоцитами и миокитными клетками. Кроме того, MSC показывает нейрозащитное воздействие на нейроны. В настоящее время многие методы нейрорегенеративного лечения МСЦ доказали свою эффективность. Исследования показали, что МСЦ в боль

Совпадение в аннотации оригинала
PURPOSE: Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) are self-regenerative and able to differentiate into multipotent stem cells. There may be different sources of mesenchymal stem cells (MSCs) involved in the repair mechanism of damaged tissues in the organism. WJ-MSCs may differentiate into osteocytes, chondrocytes, adipocytes, and myocyte cel…

Transcriptomic integration and ligand-receptor crosstalk reveal the underlying molecular mechanisms between hip cartilage and subchondral bone in osteonecrosis of femoral head.

Osteonecrosis of femoral head (ONFH) is characterized not only by ischemic bone tissue necrosis but also by cartilage degeneration, which pl … asts in the subchondral bone, such as FN1, MMP2, and FGF1. Conversely, ligands from osteoblasts and osteocytes in the subchondral bone frequently paired with chondrocyte receptors, including FN1, COL1A1, and SEMA7A. At the protein level, we identified thirteen ligands and one receptor, with COL3A1 being the most highly expressed ligand and CD82 the only diff …

Effect of Zinc Supplementation on Altered Calcium Homeostasis, Parathyroid Gland, Bone, and Skeletal Muscle Histology Induced by Subchronic Oral Exposure to Glyphosate-Based Herbicide (GOBARA®) in Wistar Rats.

OBJECTIVES: The study was carried out to assess the effect of zinc supplementation on changes in calcium homeostasis, and parathyroid gland, … e was not observed after comparing G1 and G2. Necrotic changes occurred in parathyroid gland cells, osteocytes, and muscle cells in G1 and G2. In ZG1 and ZG2, significant (P > .05) variations in the parameters were not observed and tissue lesions were absent. CONCLUSION: Subchronic GBH exposure impaired calcium homeostasis observed as hypocalcemia, hypovita …

Fenugreek Seed Ethanolic Extract Improves Alveolar Bone Parameters by Attenuating Inflammation in Ovariectomized Rats.

INTRODUCTION: Alveolar bone residual ridge resorption remains a major challenge for dental implant placement in patients with edentulism. Fe … attenuated by reductions in IL-6 and sclerostin, and an increase in TGF-β1. The maturation of bone osteocytes increased along with the increase in ER-α expression and ratio of OPG/RANKL.

Deep coverage and quantification of the bone proteome provides enhanced opportunities for new discoveries in skeletal biology and disease.

Dysregulation of cell signaling in chondrocytes and in bone cells, such as osteocytes, osteoblasts, osteoclasts, and an elevated burden of senescent cells in cartilage and bone, are implicated in osteoarthritis (OA). Mass spectrometric analyses provides a crucial molecular tool-kit to understand complex signaling relationships in age-related diseases, such as OA. Here we introduce a novel mass spectrometric workflow to promote proteomic studies of bone. This workflow uses highly specialized steps, including extensive overnight demineralization, pulverization, and incubation for 72 h in 6 M gua…