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Cryopreserved Off-the-Shelf Allogeneic Adipose-Derived Stromal Cells for Therapy in Patients with Ischemic Heart Disease and Heart Failure-A Safety Study.

The present first-in-human clinical trial evaluated the safety and feasibility of a newly developed and cryopreserved Cardiology Stem Cell Centre adipose-derived stromal cell (CSCC_ASC) product from healthy donors for intramyocardial injection in ten patients with ischemic heart disease and ischemic heart failure (IHF). Batches of CSCC_ASC were isolated from three healthy donors by liposuction from abdominal adipose tissue. Adipose mesenchymal stromal cells were culture expanded in bioreactors without the use of animal constituents, cryopreserved, and stored in vials in nitrogen dry-storage co

The Center for HIV/AIDS Vaccine Immunology (CHAVI) multi-site quality assurance program for cryopreserved human peripheral blood mononuclear cells.

The Center for HIV/AIDS Vaccine Immunology (CHAVI) consortium was established to determine the host and virus factors associated with HIV transmission, infection and containment of virus replication, with the goal of advancing the development of an HIV protective vaccine. Studies to meet this goal required the use of cryopreserved Peripheral Blood Mononuclear Cell (PBMC) specimens, and therefore it was imperative that a quality assurance (QA) oversight program be developed to monitor PBMC samples obtained from study participants at multiple international sites. Nine site-affiliated laboratorie

Application of cryopreserved vein grafts as a conduit between the coronary vein and liver graft to reconstruct portal flow in adult living liver transplantation.

Adult-to-adult living donor liver transplantation is an alternative to donation from a deceased individual, and can help relieve the shortage of liver donations available for adult patients in Asian countries. When transplant candidates have thrombosis and deterioration of the portal vein, living donor liver transplantation is relatively contraindicated because portal veins in the grafts are short and vein grafts may not be available to reconstruct the portal vein. From June 2003 to May 2007, 82 adult living donor liver transplantations were performed at Chang-Gung Memorial Hospital. Three pat

Assessment of selective homing and contribution to vessel formation of cryopreserved peripherally injected bone marrow mononuclear cells following experimental myocardial damage.

In view of a potential clinical use we aimed this study to assess the selective homing to the injured myocardium and the definitive fate of peripherally injected labeled and previously cryopreserved Bone Marrow Mononuclear cells (BMMNCs). The myocardial damage (cryoinjury) was produced in 59 rats (45 treated, 14 controls). From 51 donor rats 4.4 x 10(9) BMMNCs were isolated and cryopreserved (slow-cooling protocols); the number of CD34+ and the viability of pooled cells was assessed by flow-cytometry analysis before and after cryopreservation and simulated delivery through a 23G needle. Seven …

Characteristics of Iberian red deer (Cervus elaphus hispanicus) spermatozoa cryopreserved after storage at 5 degrees C in the epididymis for several days.

The aim of this study was to assess the influence of prolonged cold storage of Iberian red deer epididymides on post-thaw sperm characterist … rmatozoa stored in the epididymis for up to 96 h did not decrease significantly (P>0.05) but, after cryopreservation, a decline in sperm motility was seen in spermatozoa stored for 48 h, or later. A slower decrease in sperm membrane and acrosome integrities after cryopreservation were seen as storage time progressed. Some differences were seen when different metho …

[Fertilization of cryopreserved and thawed human oocytes (Cryo-Oo) by injection of spermatozoa (ICSI)--medical management of sterility and case report of a twin pregnancy].

It is reported on a twin pregnancy and parturition of a healthy girl and a healthy boy following the treatment of the mother by intracytoplasmic injection of spermatozoa (ICSI) into cryopreserved and thawed human oocytes (Cryo-Oo). The couple suffered from a primary sterility because of a severe subfertility of the husband and oligomenorrhea with anovulation of the wife. Before the couple contacted our center for the first time, several pretreatments had been carried out, e.g. intrauterine inseminations (IUI) with husband's and donor sperm and one cycle of in vitro fertilization (IVF) with int

Sperm cryopreservation of sex-reversed rainbow trout (Oncorhynchus mykiss): incorporation of amino acids and anti-oxidants to the extender media to improve the anti-oxidant system.

Anti-oxidants are vital for protecting sperm and can mitigate the negative effects associated with cryopreservation. This study was conducted to evaluate the protective effects of adding anti-oxidants (vitamins and amino acids) to extender in sex-reversed rainbow trout (Oncorhynchus mykiss) sperm. The collected sperm was diluted at the ratio of 1 : 5 by the extenders supplemented with different anti-oxidants including 1.00 mM of ascorbic acid and L-tryptophan, 2.00 mM of cysteine and α-tocopherol, and their combination. After dilution, the semen was aspirated into 0.50 mL straws, and the straw…

Cryopreservation of mesenchymal stem/stromal cells using a DMSO-free solution is comparable to DMSO-containing cryoprotectants: results of an international multicenter PACT/BEST collaborative study.

BACKGROUND AND AIM: An essential aspect of ensuring availability and stability of mesenchymal stem/stromal cells (MSCs) products for clinical use is that these cells are cryopreserved before individual infusion into patients. Currently, cryopreservation of MSCs involves use of a cryoprotectant solution containing dimethyl sulfoxide (DMSO). However, it is recognized that DMSO may be toxic for both the patient and the MSC product. In this Production Assistance for Cellular Therapies (PACT) and Biomedical Excellence for Safer Transfusion (BEST) Collaborative study, we compared a novel DMSO-free s…

Comparative analyses of physicochemical and volatile flavor characteristics in hooked, trawl-net, and radar-net hairtail (Trichiurus haumela) muscles during long-term cryopreservation at -18°C.

Chemical analysis showed that pH, b* values, myosin turbidity, carbonyl content, and surface hydrophobicity elevated in hooked, trawl-net, and radar-net hairtail (Trichiurus haumela, HH, TH, and RH) muscles with the prolonged cryopreservation time (-18℃, 120 d). In contrast, L*, a* values, textural properties, and myosin solubility existed decreasing trends. Microstructural results showed that freezing resulted in disordered myofibrils, decreased collagen fibers, widened myofibrillar space, and increased fragmentation in hairtail muscles. Furthermore, volatile flavor analysis suggested that al…

Effect of Stem Cell Cryopreservation on Day 60 Donor Chimerism in Unrelated Donor Allogeneic Stem Cell Transplantation using Reduced Intensity Conditioning and ATG-based GVHD Prophylaxis.

BACKGROUND: Cryopreservation of hematopoietic stem cell grafts has become more widely adopted. The impact of cryopreservation on clinical outcomes remains ambiguous with existing studies limited by retrospective designs, and heterogeneity in transplant protocol. The implications of cryopreservation of grafts on donor chimerism is also not well researched. METHODS: We retrospectively analysed 154 adults undergoing unrelated donor allogeneic HCT with reduced-intensity conditioning and ATG-based GVHD prophylaxis. Patients received either cryopreserved (n=85) or fresh (n=69) peripheral blood stem…

The effects of mitochondria-targeted (MitoQ and Mito-TEMPO) and untargeted (SOD and CoQ10) antioxidants on ram's sperm quality and fertility potential during the cryopreservation process.

Sperm cryopreservation is a widely used procedure for storing donor semen prior to artificial insemination. However, spermatozoa experience biochemical and functional changes during cryopreservation. The current study aimed to compare the effects of mitochondrial-targeted (MitoQ and Mito-TEMPO) and untargeted (SOD and CoQ10) antioxidants on the quality traits of ram sperm and reproductive performance. Semen samples were collected and diluted in extenders as follows: the first group served as the control, while the other groups were supplemented with 100 U/mL superoxide dismutase (SOD), 1 μM Co…

Factors associated with decision outcomes along the planned oocyte cryopreservation pathway in a cohort of women who sought consultation before and during the COVID-19 pandemic.

PURPOSE: To identify factors associated with three decision outcomes along the planned oocyte cryopreservation (POC) pathway: fertility assessment completion, POC uptake, and multiple POC cycles uptake. METHODS: A single-site retrospective cohort study of 425 patients who sought POC consultation from 2018-2022 before and during the COVID-19 pandemic. RESULTS: The mean age of patients at consultation was 35.2 ± 4.0. 73% (n = 310) of the new consultations occurred since the COVID-19 pandemic. 335 patients completed fertility assessment following consultation. Of the 335 patients (78.8%) comple…

Dose-dependent effects of Nymphaea lotus extract in semen extenders on sperm quality and fertility in Thai native roosters during cooled and cryopreserved storage.

This study evaluated the dose-dependent effects of Nymphaea lotus extract supplementation in semen extenders on sperm quality and fertility in Thai native roosters under cooled and cryopreserved storage conditions. Phytochemical analysis revealed that the ethanolic extract contained high levels of total phenolics (596 mg/g DW) and proanthocyanidins (295 mg/g DW), along with flavonoids and tannins. In vitro antioxidant assays demonstrated strong radical-scavenging and ferric-reducing activities, with IC₅₀ values comparable to standard antioxidants, and significant protection against AAPH-induce

Romiplostim N01 accelerates platelet engraftment in autologousstem cell transplantation using non-cryopreserved peripheral blood stem cells for plasma cell neoplasms.

BACKGROUND: Delayed platelet engraftment remains a major limitation of autologous stem cell transplantation (ASCT) for plasma-cell neoplasms. Romiplostim N01, a thrombopoietin receptor agonist, may enhance early megakaryocytic recovery, while the use of non-cryopreserved peripheral blood stem cells (PBSCs) eliminates dimethyl-sulfoxide-related toxicity and reduces procedural cost. METHODS: This retrospective study evaluated 15 patients receiving non-cryopreserved PBSCs and early Romiplostim N01 after ASCT and compared them with 21 historical controls who received cryopreserved PBSCs and recom

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… Non-cryopreserved PBSCs infusion eliminates DMSO-associated toxicities, supports more predictable scheduling, and reduces dependence on cryopreservation facilities ( 34 ). … Cryopreserved versus non-cryopreserved stem cell autografts in multiple myeloma a restrospective cohort study. Bone Marrow Transplant. (2022) 57:1313–8. doi: 10.1038/s41409-022-01718-2. PMID: 33. …

Non-cryopreserved Kx negative packed red cell concentrates to support hematopoietic stem cell transplantation in McLeod contiguous gene deletion syndrome.

Transfusion management of Kx- individuals with McLeod phenotype (MLP) is highly challenging, particularly as cryopreservation affects red blood cell (RBC) concentrate quality. We developed a concept to provide non-cryopreserved Kx- RBCs over the complete period of hematopoietic stem cell transplantation (HSCT) for treatment of X-linked chronic granulomatous disease (X-CGD) with MLP. An infant with a large deletion affecting 12 protein-coding genes, including DMD, PRRG1, LANCL3, XK, CYBB, and DYNLT3, leading to CGD, Duchenne muscular dystrophy, and MLP, was scheduled for HSCT with the need of K…

A Retrospective Analysis of Fresh versus Cryopreserved Allogenic Bone Marrow Transplant within a Pediatric Population: A Change in Practice Due to the COVID-19 Pandemic.

BACKGROUND: Several adult studies show mixed reports in clinical outcomes between cryopreserved and fresh stem cell products, with majority reporting no significant differences and others report that there are differences in outcomes. There is limited literature reporting its impact on outcomes in pediatric hematopoietic cell transplantation (HSCT). OBJECTIVE: To compare clinical outcomes between fresh vs cryopreserved stem cell treatment in pediatric HSCT. STUDY DESIGN: A retrospective chart review was conducted on allogenic HSCT at Phoenix Children's Hospital between January 1, 2016, and M

Erratum: Tips and tricks and clinical outcome of cryopreserved human amniotic membrane application for the management of medication- related osteonecrosis of the jaw (MRONJ): A pilot study.

[This corrects the article DOI: 10.3389/fbioe.2022.936074.].

Use of Cryopreserved Human Umbilical Cord and Amniotic Membrane Allograft and Portable Negative Pressure Therapy in Dehisced Giant Omphalocele Repair: A Case Study.

BACKGROUND: A giant omphalocele is a rare congenital abdominal wall defect that measures more than 6 to 8 cm in any dimension. The child's abdominal visceral contents as well as the liver which are both covered by the amniotic sac protrude outside the abdomen through the umbilicus. Consequently, closing this defect is extremely challenging due to the risk for wound dehiscence. CASE: Baby C, a 1-year-old male infant, developed a dehisced abdominal wound after secondary repair of a giant omphalocele. Escharification of the amniotic sac was achieved with silver products, followed by intra-abdomi

Stage IV Perineal Pressure Ulcers in Immobile Patients Treated With Surgical Flap Closure Augmented With Cryopreserved Placental Membrane Containing Viable Cells.

INTRODUCTION: Surgical closure of late-stage pressure ulcers (PUs) poses challenges in the immobilized population due to the high rate of complications, including infection, dehiscence, and recurrence. Muscle flap closure is the standard treatment for chronic, late-stage (stage 4) PUs, characterized by the European Pressure Ulcer Advisory Panel and National Pressure Ulcer Advisory Panel as full-thickness tissue loss with exposure of bone, tendon, or muscle. OBJECTIVE: The aim of this study is to evaluate the outcomes associated with the use of a cryopreserved placental membrane containing via