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EBNA2 и EBNA-LP: Самые славные протеины.

EBNA2 и EBNA-LP являются самыми ранними вирусными белками запоздалости после заражения клеток B вирусом Эпштейн-Барр (EBV) и имеют важное значение для клеточной трансформации и увековечения. Оба белка совместно выражаются во время состояния запоздалости IIb и III и демонстрируют временное регулирование от вирусных пропагандистов Wp до Cp в течение первых 24 часов инфекции. Недавние достижения коренным образом изменили наше понимание механизмов действий EBNA2, продемонстрировав ее способность пройти жидко-жидкостную фазу разделения для образования ядерных конденсатов, которые реорганизуют хост-

Совпадение в аннотации оригинала
EBNA2 and EBNA-LP are the earliest expressed viral latency proteins following Epstein-Barr virus (EBV) infection of B cells and are essentia … d our understanding of EBNA2's mechanisms of action, revealing its ability to undergo liquid-liquid phase separation to form nuclear condensates that reorganize host chromatin topology and create accessible chromatin domains. EBNA2 functions through sophisticated partnerships with cellular transcription factors including RBP-Jκ and EBF1, exploiting preexisting B c …

The nuclear condensates of ESE3/EHF induce cellular senescence without the associated inflammatory secretory phenotype in pancreatic ductal adenocarcinoma.

Senescent cells are in a stable state of cell cycle arrest, leading to a natural barrier to tumorigenesis. Senescent cells secrete a pool of … Furthermore, high throughput drug screening identified that Bilobetin could effectively promote the phase separation of EHF, which could further induce tumoral senescence but without SASP. In vivo, preclinical translational research uncovered that Bilobetin could ameliorate immunosuppressive tumor microenvironment (TME) and sensitize PDAC to anti-PD-1 therapy. Ove …

Novel HPTLC method for simultaneous estimation from ternary mixture of chlorpheniramine maleate, dextromethorphan hydrobromide and phenylephrine hydrochloride in syrup formulations.

OBJECTIVES: A synergic antihistamine, cough suppressant, and decongestant combination of chlorpheniramine, dextromethorphan, and phenylephrine is used to treat acute respiratory infections caused by seasonal viruses. The effective qualitative and quantitative methods require the simultaneous measurement of a ternary combination in the pharmaceutical syrup dosage form. Therefore, a new, simple, fast and robust high performance thin layer chromatographic (HPTLC) method has been developed and validated for chlorpheniramine maleate (CPM), dextromethorphan hydrobromide (DEXO) and phenylephrine hydr

Simultaneous analysis of antihyperglycemic small molecule drugs and peptide drugs by means of dual liquid chromatography high-resolution mass spectrometry.

OBJECTIVES: The study aimed to evaluate dual liquid chromatography (LC) coupled to high-resolution mass spectrometry (HRMS) for the simultaneous analysis of small and large molecule drugs by development and application of a validated bioanalytical method. METHODS: The oral antihyperglycemic drugs (OAD) dapagliflozin, empagliflozin, glibenclamide, glimepiride, metformin, pioglitazone, repaglinide, saxagliptin, sitagliptin, and vildagliptin, as well as the antihyperglycemic peptides exenatide, human insulin, insulin aspart, insulin degludec, insulin detemir, insulin glargine, insulin glulisine,

Simultaneous determination of dissolved organic nitrogen, nitrite, nitrate and ammonia using size exclusion chromatography coupled with nitrogen detector.

Accurate quantification of dissolved organic nitrogen (DON) has been a challenge due to the cumulative analytical errors in the conventional method via subtracting dissolved inorganic nitrogen species (DIN) from total dissolved nitrogen (TDN). Size exclusion chromatography coupled with an organic nitrogen detector (SEC-OND) has been developed as a direct method for quantification and characterization of DON. However, the applications of SEC-OND method still subject to poor separations between DON and DIN species and unsatisfied N recoveries of macromolecules. In this study, we packed a series

Development, validation, and application of an RP-HPLC method for concurrent quantification of Minoxidil and Finasteride in a topical solution for hair regrowth.

A topical solution comprising of Minoxidil (MXL) and Finasteride (FNS) for alopecia is formulated in the present work, which essentially contains a lipid-Lauroglycol FCC as a penetration enhancer. The objective of the proposed work was to develop a rapid, simple, and robust reverse phase high performance liquid chromatographic (RP-HPLC) method to determine MXL and FNS in the said formulation. Herein, the chromatographic conditions were optimized based on the theoretical principles of separation and physicochemical properties such as pKa and log P of both the Active Pharmaceutical Ingredients (

Engineered nanotopography on electrospun PLLA microfibers modifies RAW 264.7 cell response.

In this study, we created a new method of electrospinning capable of controlling the surface structure of individual fibers (fiber nanotopography). The nanotopographical features were created by a phase separation in the fibers as they formed. To control the phase separation, a nonsolvent (a chemical insoluble with the polymer) was added to an electrospinning solution containing poly-l-lactic acid (PLLA) and chloroform. The nanotopography of electrospun fibers in the PLLA/chloroform solution was smooth. However, adding a small weight (<2% of total solution) of a single nonsolvent (water, ethan…

Interface-induced crystalline ordering and favorable morphology for efficient annealing-free poly(3-hexylthiophene): fullerene derivative solar cells.

A simple approach to fabricate high-efficiency annealing-free poly(3-hexylthiophene): [6,6]-phenyl C(61)-butyric acid methyl ester (P3HT:PCBM) solar cells is reported by using p-type CuI to substitute PEDOT:PSS as anode buffer layer. It is found that the P3HT:PCBM blend films deposited on CuI surface show different orientation of crystalline P3HT domains and phase separation from those deposited on PEDOT:PSS surface. A nanoscale phase separation of P3HT and PCBM with domain sizes about 10-30 nm is formed for the P3HT:PCBM blend films deposited on CuI surface. Absorption and grazing incidence X…

Ammonia-Resistant Carbon-Encapsulated Cobalt Catalyst for Electrocatalytic Nitrate Reduction to Volatile Ammonia.

Electrocatalytic nitrate reduction to ammonia presents a promising route for sustainable nitrogen utilization; however, current efforts remain largely confined to optimizing synthesis efficiency, with insufficient attention paid to the concurrent challenge of ammonia separation-a key bottleneck for scalable deployment. Here, we report carbon-shell-encapsulated cobalt nanoparticles (Co@C), which uniquely enable integrated ammonia synthesis and in situ gas-phase purification under strongly alkaline conditions. In both sequencing batch and continuous-flow electrolyzers, Co@C delivers a robust nit

Engineering Inner-Surface Electrostatic Potential of Robust B←N Dative Cages for Efficient Benzene/Cyclohexane Separation.

Benzene (Bz) and cyclohexane (Cy) pose formidable separation challenges owing to their nearly identical physicochemical properties, while their inherent electronic structural differences offer a viable avenue for selective discrimination. Herein, we report an efficient separation strategy via precise electrostatic potential engineering of confined cage cavities to realize electronic structure matching with the aromatic π-system of Bz. Guided by this principle, a series of robust trigonal prismatic B←N dative cages with continuously tunable inner-cavity electrostatic potential distributions wer

Reversible Switching and Recycling of Thermoresponsive 1,2,4-Triazolium-Based Poly(ionic liquid) Catalysts for Porous Organic Cage Synthesis in Organic Media.

Homogeneous catalysts of high activity and selectivity often face challenges in the separation from feedstocks and products after reactions. In contrast, heterogeneous catalysts are easier to separate, usually at the cost of compromised catalytic performance. By designing catalysts capable of switching between homogeneous and heterogeneous states for catalysis and separation, the merits of both could be synergistically combined. In this study, a thermoresponsive 1,2,4-triazolium-based poly(ionic liquid) (PIL) was applied as a temperature-switchable organocatalyst for the controlled synthesis o

Direct Z-scheme 1D/2D WO2.72/ZnIn2S4 hybrid photocatalysts with highly-efficient visible-light-driven photodegradation towards tetracycline hydrochloride removal.

There are increasing environmental concerns of serious pollution from emission of antibiotic wastewater. Herein, a series of direct Z-scheme WO2.72/ZnIn2S4 (WOZIS) hybrid photocatalysts composed of one-dimensional (1D) WO2.72 (WO) nanorods and two-dimensional (2D) ZnIn2S4 (ZIS) nanosheets have been designed and constructed for tetracycline hydrochloride (TCH) degradation without presence of solid-state electron mediators. The crystalline phase, chemical composition, morphology, optical properties and photocatalytic activity of the as-prepared samples were characterized by the XRD, XPS, SEM, HR

Stable isotope dimethyl labeling combined with LTQ mass spectrometric detection, a quantitative proteomics technology used in liver cancer research.

Liver cancer is a common malignant disease, with high incidence and mortality rates. The study on the proteomics of liver cancer has attracted particular attention. The quantitative study method of proteomics depends predominantly on two-dimensional (2D) gel electrophoresis. In the present study we reported a rapid and accurate proteomics quantitative study method of high repeatability that includes the use of stable isotope labeling for the extraction of proteins and peptides via enzymolysis to achieve new type 2D capillary liquid chromatography-mass spectrometry separation using the separati

Determination of tetracyclines in pig and other meat samples using liquid chromatography coupled with diode array and tandem mass spectrometric detectors.

Two high performance liquid chromatographic methods (HPLC-DAD and LC-MS/MS) were developed to analyze tetracycline (TC) residues in pig meat (pork) samples. The method involved a sample preparation using a solid-liquid extraction (SLE) by McIlvaine buffer, followed by a solid-phase extraction (SPE) clean-up using Strata-XL cartridges. The developed sample clean-up resulted in a selective chromatogram in the HPLC-DAD separation and a reduced matrix effect (ME) in LC-MS/MS analysis. Moreover, HPLC columns packed with core-shell particles were tested for separation, which further enhanced the sen

[Identification of the related substances in docetaxel injection by LC-MS/MS].

The related substances in docetaxel injection were identified by LC-MS/MS. Ethyl acetate was used to extract the injection to remove the pharmaceutical excipients. HPLC separation was carried out on a Hedera ODS-2 column (150 mm x 2.1 mm, 5 microm) with a mobile phase consisting of acetonitrile - 0.1% acetate acid aqueous solution (40: 60). Electrospray ionization source was set in the positive mode for the LC-ESI-MS/MS, and the ion monitoring modes were full scan and product ion scan. According to the mass spectra of the related substances, the fragment profiles were explained, and the chemic

Pressurized liquid extraction of coumarins from fruits of Heracleum leskowii with application of solvents with different polarity under increasing temperature.

Coumarins are nowadays an important group of organic compounds from natural sources that are useful in a number of fields. Because they possess different pharmacological properties, finding the proper extraction conditions for their separation from plant matrices is a very important step. In this report Pressurized Liquid Extraction (PLE) under different temperature conditions and with different types of extraction solvents were tested. As a matrix, fruits of Heracleum leskowii have been used. A simple reverse phase high-performance liquid chromatographic method (RP-HPLC) coupled with a photod

Column switching liquid chromatography and post-column photochemically fluorescence detection to determine imidacloprid and 6-chloronicotinic acid in honeybees.

The determination of imidacloprid and its main metabolite (6-chloronicotinic acid) in honeybees was performed by liquid chromatography with post-column photochemical derivatisation in alkaline medium and fluorescence detection. The compounds were extracted from honeybees with acetone under ultrasound conditions prior to liquid-liquid partition with dichloromethane. The separation of extract components was performed using a 50 mm x 4.6 mm i.d. short column packed with 5 microm Aquasil C(18) using an acetonitrile:water gradient program as mobile phase. Injection of samples in 0.1 mol L(-1) H(3)P

Use of geotextile tubes with chemical amendments to dewater dairy lagoon solids.

Three geo-textile filtration tubes were used to dewater lagoon solids from a first stage dairy lagoon using chemical amendments (aluminum sulfate and a polymer) to enhance the separation process. This experiment had previously been done without chemical amendment. The chemical amendments speeded the dewatering process so that filling could be accomplished sooner, and also increased the removal rate of nutrients, especially phosphorus into the solid phase. Chemically amended slurry was pumped from the lagoon into the tube with the filtered liquid seeping from the tube and returning to the lagoo

Analysis of residues of imidacloprid in tobacco by high-performance liquid chromatography with liquid-liquid partition cleanup.

A practical method for analysis of residues of imidacloprid in baked tobacco leaves has been developed using HPLC with liquid-liquid partition clean-up. Imidacloprid was extracted with ethyl acetate under ultrasound, and cleaned up by liquid-liquid partition with 50 g litre(-1) aqueous sodium chloride followed by dichloromethane. The water-soluble and fat-soluble components in tobacco, which interfere with imidacloprid in HPLC, were removed. The separation was performed on a Supelco LC-18 column (250 mm x 4.6 mm ID) with a mobile phase of acetonitrile + 5 mM ammonium acetate (20 + 80 by volume

Sequence of centromere separation: kinetochore formation and DNA replication in dicentric chromosomes showing premature centromere separation in rat cerebral cells.

A subpopulation of rat cerebral endothelial cells, designated B1, exhibits an array of multicentric chromosomes. Because of the formation of bridges at anaphase, this cell population produced new types of multicentrics at every cell division. These chromosomes showed kinetochore proteins at every centromeric site and all centromeric regions replicated their DNA at the end of the S phase, more or less simultaneously. A new subpopulation of cells, designated B2, obtained from the original sample frozen at Wayne State University displayed several dicentrics. In contrast to B1 these chromosomes ex