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[Occupational brucellosis in slaughtering of sheep and goats: study of five cases from a municipal abattoir in south-eastern Sicily].

BACKGROUND: Brucellosis is a world-wild zoonosis of bacterial origin. In Italy, where reporting of the disease is mandatory, the incidence is 2.1 cases every 100,000 inhabitants, but tends to be higher in southern regions. However, the incidence decreased from 1999 to 2002. CASES: Five cases of occupational brucellosis, four slaughter-house operators and one veterinary surgeon, were reported in a slaughter-house in south-eastern Sicily employing 15 people. RESULTS: A study of the slaughtering process for sheep and goats led to identification of 23 different operations, some of which involved

Clinical Coxiella burnetii infection in sable and roan antelope in South Africa.

Various zoonotic microorganisms cause reproductive problems such as abortions and stillbirths, leading to economic losses on farms, particularly within livestock. In South Africa, bovine brucellosis is endemic in cattle, and from 2013-2018, outbreaks of Brucella melitensis occurred in sable. Coxiella burnetii, the agent responsible for the zoonotic disease known as Q-fever and/or coxiellosis, also causes reproductive problems and infects multiple domestic animal species worldwide, including humans. However, little is known of this disease in wildlife. With the expansion of the wildlife industr…

Fatal Brucellosis Infection in a Liver Transplant Patient: A Case Report and Review of the Literature.

Brucellosis is the most common zoonosis, particularly in developing countries. The true incidence of human brucellosis is unknown. The WHO p … ll count of 18990/uL and creatine phosphokinase 10302 U/L. Routine blood cultures were positive for Brucella melitensis. The patient reported consumption of unpasteurized bovine milk. He was treated with doxycycline and ciprofloxacin. Despite antibiotic management, after one month of hospitalization and in the context of septic shock with multiorgan failure, the pati …

[Evaluation of diagnostic value of Brucellacapt test in brucellosis].

Although standard tube agglutination (STA) test is the most preferred method for the serodiagnosis of brucellosis, it may yield false negati … in the serodiagnosis of brucellosis. Twenty-five brusellosis patients whose blood cultures yielded Brucella melitensis growth, and 31 healthy subjects as controls, were included to the study. Serum samples obtained from all of the 56 subjects have been searched by STA, Coombs' and Brucellacapt methods, and threshold titre was accepted as 1/160. As a result, 40% (10/ …

Expression cloning and biochemical characterization of a Rhizobium leguminosarum lipid A 1-phosphatase.

Lipid A of Rhizobium leguminosarum, a nitrogen-fixing plant endosymbiont, displays several significant structural differences when compared … hologs of LpxE are present in some intracellular human pathogens, including Francisella tularensis, Brucella melitensis, and Legionella pneumophila.

The use of mutants of Escherichia coli for the identification of human lymphocyte subpopulations in blood smears.

In previous work we have shown that some bacteria can bind to human lymphocytes and can be used to identify lymphocyte subpopulations in conventionally stained blood smears. These bacteria are of different species or genera, which makes it difficult to study the binding mechanism. Also, the main marker for B cells, Brucella melitensis, is of very small size and highly pathogenic. Here we show that B cells as well as some of the T cell subpopulations can be identified by different mutants obtained from a strain of an Escherichia coli. Two procedures were used to generate mutants. First, E. coli…

Molecular detection of Coxiella burnetii in vaginal and rectal swab samples of small ruminants from Kermanshah Province, western Iran.

BACKGROUND AND PURPOSE: Coxiella burnetii is a zoonotic bacterial pathogen that causes Q fever in humans and coxiellosis in livestock. It re … gests other pathogens may contribute to reproductive losses. Multi-pathogen surveillance, including Brucella melitensis, Chlamydia abortus, and longitudinal sampling are recommended to enhance detection accuracy and elucidate the causes of abortion in livestock.

TRANQUIL уменьшает аутоиммунную демииляцию и подавляет нейроинфламматический сигнал в экспериментальном аутоиммунном энцефаломиелите.

Нейроинфламмация, движущаяся автоматически реактивными клетками Т и активированными глиальными популяциями, лежит в основе патогенеза множественного склероза (MS), однако нынешняя иммунодуляторная терапия редко восстанавливает долгосрочную иммунную терпимость и часто вызывает широкое иммуноусилительное воздействие. Ранее мы сконструировали слабый штамм брюселлы ΔvjbR, выражающий tnaA, позволяющий создать производство триптофана метаболита индола, модулятора активации антигенной клетки (APC) и дифференциации Т-клеток. Здесь мы проверили, улучшит ли этот штамм (TRANQUIL - Technology for Reprogra

Совпадение в аннотации оригинала
Neuroinflammation driven by autoreactive T cells and activated glial populations underlies the pathogenesis of multiple sclerosis (MS), yet current immunomodulatory therapies rarely restore durable immune tolerance and often cause broad immunosuppression. We previously engineered an attenuated Brucella melitensis ΔvjbR strain expressing tnaA, enabling consti…

Optimization of pathogen detection in abscess specimens: a 6-year retrospective study.

BACKGROUND: This study aimed to evaluate the impact of optimized diagnostic protocols on pathogen detection rates in abscess specimens. MET … ield), including anaerobes (33.7%), smear-positive organisms (32.2%), acid-fast bacilli (6.9%), and Brucella melitensis (1.5%). Anatomic distribution analysis revealed perianal abscesses (358 cases, 407 isolates) predominantly associated with E. coli (51.8%), K. pneumoniae (14.7%), and Streptococcus spp. (15.7%), followed by maxillofacial infections (244 cases, 297 i …

Liposomal Amphotericin B-induced Cardiac Arrhythmias in Infantile Visceral Leishmaniasis: A Case Report.

BACKGROUND: Visceral Leishmaniasis is a systemic vector-borne infection with a poor prognosis if not treated. Classical antiparasitic therapy with liposomal amphotericin B (LAmB) is effective, but occasionally not well-tolerated. CASE PRESENTATION: An 11-month-old male infant was admitted to our hospital due to prolonged fever, following an RSV infection. The patient had pale skin and splenomegaly, but was hemodynamically stable. An infectious cause was investigated through serology for Leishmania species , Brucella melitensis , Toxoplasma gondii , EBV, CMV, PB19 and Salmonella species . Afte…

In Silico Evaluation of Lawsonia intracellularis Genes Orthologous to Genes Associated with Pathogenesis in Other Intracellular Bacteria.

Proliferative enteropathy is an enteric disease caused by the bacterium Lawsonia intracellularis, which affects several species of domestic and wild animals. The mechanisms underlying the mechanisms employed by L. intracellularis to cause host cell proliferation are poorly understood, mostly because this bacterium is extremely difficult to isolate and propagate in vitro. Comparative genomics methods for searching for genes orthologous to genes known to be associated with pathogenesis allow identification of genes potentially involved in pathogenesis by the pathogen of interest. The goal of thi

A Retrospective Study of 39 Patients Treated With Anterior Approach of Thoracic and Lumbar Spondylodiscitis: Clinical Manifestations, Anterior Surgical Treatment, and Outcome.

The aim of this study is to report our 39 patients treated with anterior debridement and autologous iliac bone grafting with or without ante … nic infection included Staphylococcus aureus (4 patients), Pseudomonas aeruginosa (3 patients), and Brucella melitensis (1 patient). Fifteen patients had thoracic involvement, 20 had lumbar involvement, and 4 had thoracolumbar junction involvement. Preoperative neurological deficits were noted in 13 of the 39 patients. In terms of Frankel grade, 8 patients have impro …

Bacteriological features of infectious spondylodiscitis at Mohammed V Military Teaching Hospital of Rabat.

To review the bacteriological features of infectious spondylodiscitis and provide recommendations for the initial therapy which remains empi … opionibacterium acnes, Staphylococcus aureus, Staphylococcus epidermidis, Corynebacterium species). Brucella melitensis was isolated in 1 case. Infections caused by pyogenic bacteria were isolated in 15 cases of which 12 (12/15) revealed simple organisms including Gram-positive cocci in 9 cases (9/12) with 3 cases of S. aureus and Gram-negative bacilli in 3 cases (3/ …

Use of shell-vial cell culture assay for isolation of bacteria from clinical specimens: 13 years of experience.

The shell-vial culture assay is performed routinely in our laboratory. Recently we revisited our experience of using the shell-vial culture … d genera, 6 Mycobacterium sp. isolates, 1 Nocardia asteroides isolate, 1 Actinomyces sp. isolate, 1 Brucella melitensis isolate, 2 Francisella tularensis isolates, 1 Mycoplasma pneumoniae isolate, and 1 Legionella pneumophila isolate. Using this protocol, we have also cultured intracellular bacteria such as Chlamydia trachomatis and we have performed the first cultur …

Spinal brucellosis in Hulunbuir, China, 2011-2016.

Purpose: To investigate the demographic, epidemiological, clinical, and laboratory characteristics; treatment options; and outcome of human … ), thoracolumbar (2.9%), and lumbosacral (2.9%). All isolates from blood culture were identified as Brucella melitensis, with 61% biovar 3 and 39% biovar 1 isolates. The antimicrobial therapy for BS lasted for at least 3 months. In the presence of paravertebral or epidural abscess, longer treatment was conducted to avoid possible sequelae. Conclusion: In endemic area …

Roles of genomic island 3 (GI-3) BAB1_0267 and BAB1_0270 open reading frames (ORFs) in the virulence of Brucella abortus 2308.

One of the properties of bacteria is their capacity to acquire large fragments of genomic DNA from other bacteria or to loose important parts of their own genome. Such fragments include genomic islands (GIs); nine GIs are present in Brucella, including genomic island 3 (GI-3), present in B. abortus, B. melitensis and B. ovis. The GI-3 have 29 open reading frames (ORFs) most of them with unknown function. Within the GI-3, the ORFs BAB1_0267 encodes a hypothetical protein sharing a SH3 domain and BAB1_270 a zinc-dependent metallopeptidase. We have obtained deletion mutants for BAB1_0267 and BAB1

Characterization of genomic island 3 and genetic variability of Chilean field strains of Brucella abortus.

One of the capabilities developed by bacteria is the ability to gain large fragments of DNA from other bacteria or to lose portions of their own genomes. Among these exchangeable fragments are the genomic islands (GIs). Nine GIs have been identified in Brucella, and genomic island 3 (GI-3) is shared by two pathogenic species, B. melitensis and B. abortus. GI-3 encodes mostly unknown proteins. One of the aims of this study was to perform pulsed-field gel electrophoresis (PFGE) on field isolates of B. abortus from Chile to determine whether these isolates are clonally related. Furthermore, we fo

Metabolic reprogramming of NK cells in chronic brucellar arthritis: role of the HIF-1α/mTOR axis.

BACKGROUND: Brucellar arthritis is the most common complication of brucellosis, and its chronic progression is closely associated with immun … phenotypes, functions, metabolic profiles, and HIF-1α/mTOR pathway activity. In vitro, heat-killed Brucella melitensis 16M (HKBA) was used to stimulate NK-92 cells and HIF-1α-knockout (KO) cell lines, with mTOR inhibitor intervention, to dissect the regulatory mechanism of the HIF-1α/mTOR axis in NK cell metabolic reprogramming. RESULTS: scRNA-seq revealed enrichme …

[Epidemic outbreak of 81 cases of brucellosis following the consumption of fresh cheese without pasteurization].

BACKGROUND: In spite of the great effort that has been made in recent years in Castilla-La Mancha to control brucelosis, a lack of awareness on the part of producers and consumers leads to major epidemic outbreak, such as the one described below. METHODS: A description of the outbreak is described and a study is conducted of cases and controls to determine the factors which are responsible for the epidemic. Unadjusted and adjusted Odds Ratios (O.R.) are obtained together with their confidence intervals, for the main epidemiological factors studied. RESULTS: A total of 81 cases of brucelosis