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INFECTION OF GUINEA PIGS BY APPLICATION OF VIRUS OF LYMPHOCYTIC CHORIOMENINGITIS TO THEIR NORMAL SKINS.

As shown in Table I, 97 guinea pigs were used in this study. Fifty-seven were exposed by placing a virus suspension on their normal skins. Of this number, 34 had screw-top capsules attached to them. Thirteen were exposed by spreading the virus suspension on their feed and cage litter. Ten were inoculated intracerebrally to establish the potency of the virus. The remaining 17 were not exposed artificially to the virus and were employed as controls to detect cross infection. Twenty-two guinea pigs, to which capsules were attached, died as a result of virus infection; 1 died of unknown causes and…

Diversity of insect-specific viruses in Culex mosquitoes from Papua New Guinea.

Insect-specific viruses (ISVs) are integral components of mosquito viromes, influencing virus evolution, vector competence, and arbovirus ecology. Papua New Guinea (PNG) is a region of exceptional biodiversity, yet ISVs in local mosquito populations remain poorly characterized. Here, we present the first comprehensive metagenomic survey of ISVs in Culex mosquitoes collected across five provinces of PNG between 2019 and 2021. Using unbiased shotgun metagenomic sequencing, 13,871 female Culex mosquitoes representing five species were analyzed across 86 samples. Approximately 1.6 billion paired-e…

[Conservative management of macrodontic cheek teeth in 34 guinea pigs (Cavia porcellus)].

OBJECTIVE: The aim of this study was to evaluate the therapeutic success of a conservative treatment approach for macrodontic molars in guinea pigs. To this end, longitudinal data from conservative management were analyzed and parameters of potential prognostic relevance were examined. MATERIAL AND METHODS: This retrospective study includes 34 guinea pigs diagnosed with macrodontic molars for the first time at the veterinary clinic Posthausen between 2019 and 2025. Diagnosis was based on macroscopic, endoscopic and radiological findings, guided by morphological criteria described in the scien…

Loop-Mediated Isothermal Amplification (LAMP) Assessment for Malaria Diagnosis in Equatorial Guinea.

Malaria diagnosis in Equatorial Guinea (EG) is based on conventional microscopy, sometimes combined with rapid diagnostic tests (RDTs). However, some laboratories, such as the Baney Research Laboratory (BRL) have implemented molecular technology for the diagnosis of infectious diseases. This study aimed to evaluate, for the first time in EG, a LAMP method for malaria diagnosis, comparing its performance with conventional microscopy performed in EG and with LAMP and PCR diagnostic methods performed at the National Centre of Tropical Medicine (NCTM), Spain. A total of 178 blood samples (includin…

Health system effectiveness of symptomatic malaria case management in Papua New Guinea.

Effective case management is crucial for malaria control efforts and is a cornerstone of malaria control programmes. Yet, although efficacious treatments exist, malaria case management often faces challenges, such as poor access to treatment providers, supply-chain issues, non-compliance with guidelines or substandard medication. In Papua New Guinea (PNG), progress in control efforts has stagnated in recent years. This study identifies barriers to and areas for improvement in malaria case management in PNG.A cascade of care model was used to estimate the health system effectiveness of malaria …

Refractive status and histological changes after posterior scleral reinforcement in guinea pig.

AIM: To investigate the refractive and the histological changes in guinea pig eyes after posterior scleral reinforcement with scleral allografts. METHODS: Four-week-old guinea pigs were implanted with scleral allografts, and the changes of refraction, corneal curvature and axis length were monitored for 51d. The effects of methylprednisolone (MPS) on refraction parameters were also evaluated. And the microstructure and ultra-microstructure of eyes were observed on the 9d and 51d after operation. Repeated-measures analysis of variance and one-way analysis of variance were used. RESULTS: The r…

Abdominal ultrasound features and reference values in healthy guinea pigs (Cavia porcellus).

BACKGROUND: This study aimed to determine the ultrasonographic features and reference values of the abdominal anatomy in guinea pigs. METHODS: A complete abdominal ultrasonographic examination was performed in 20 adults and 20 young guinea pigs. The thickness of the wall of the gallbladder, stomach, duodenum, caecum, colon and urinary bladder (UB) was measured. Also, the adrenal glands (AGs) (width of the cranial and caudal poles, length), kidneys (length, width, height), ovaries (length, width), testes (length, width), uterus (width) and seminal glands (width) and the thickness of the spleen…

Endurance training-induced cardiac remodeling in a guinea pig athlete's heart model.

Besides the health benefits of regular exercise, high-level training-above an optimal level-may have adverse effects. In this study, we investigated the effects of long-term vigorous training and its potentially detrimental structural-functional changes in a small animal athlete's heart model. Thirty-eight 4-month-old male guinea pigs were randomized into sedentary and exercised groups. The latter underwent a 15-week-long endurance-training program. To investigate the effects of the intense long-term exercise, in vivo (echocardiography, electrocardiography), ex vivo, and in vitro (histopatholo…

Флуазурон в устной форме применяется к подопытным свинкам: фармакокинетика и эффективность против скульптумы Амблиоммы.

КОЛИЧЕСТВО: Бразильская пятнистая лихорадка (БФС), самая смертоносная болезнь, переносимая клещами в Западном полушарии, вызвана бактерией Рикетсия Рикетсия Рикеттсии и передается укусом скульптума Амблиоммы. Капибара считаются главными хозяевами этого клеща и усилителями Р. Рикеттси, генерирующими новые зараженные линии А. скульптума в районах, эндемичных для БФС. В целях определения возможного режима лечения для контроля тика А. скульптума в капибарах цель этого исследования заключалась в установлении эффективной дозы флуазурона (FLU) для контроля А. скульптума личинки в искусственно заражён

Совпадение в аннотации оригинала
BACKGROUND: Brazilian spotted fever (BSF), the most lethal tick-borne disease in the Western Hemisphere, is caused by the bacterium Ricketts … establish an effective fluazuron (FLU) dose to control A. sculptum larvae in artificially infested guinea pigs. METHODS: In Study I (pharmacokinetic and pharmacodynamic analysis), 24 guinea pigs were divided into four equal groups: control group (CG; untreated) and treated groups receiving FLU administered by gavage in three doses: G1-1 mg/kg, G2-5 mg/ …

Combined Epidemiologic and Entomologic Survey to Detect Urban Malaria Transmission, Guinea, 2018.

Malaria incidence is generally lower in cities than rural areas. However, reported urban malaria incidence may not accurately reflect the level of ongoing transmission, which has potentially large implications for prevention efforts. To guide mosquito net distribution, we assessed the extent of malaria transmission in Conakry, Guinea, in 2018. We found evidence of active malaria transmission.

Polymerized Hemoglobin With Increased Molecular Size Reduces Toxicity in Healthy Guinea Pigs.

Hemoglobin (Hb)-based oxygen (O2) carriers (HBOCs) have been developed as an alternative to red blood cells (RBCs) for use in transfusion me … toxicity of small-MW Polymerized bovine Hb (SPolyHb) to large-MW Polymerized bovine Hb (LPolyHb) in guinea pigs, since they lack the ability to synthesize vitamin C and are more sensitive to oxidative stress than other preclinical animal models. The two PolyHbs used in this study have similar molecular diameters (72 and 69 nm, respectively), but the SPol …

Increased Choroidal Blood Perfusion Can Inhibit Form Deprivation Myopia in Guinea Pigs.

PURPOSE: In guinea pigs, choroidal thickness (ChT) and choroidal blood perfusion (ChBP) simultaneously decrease in experimental myopia, and both increase during recovery. However, the causal relationship between ChBP and myopia requires further investigation. In this study, we examined the changes of ChBP with three different antimyopia treatments. We also actively increased ChBP to examine the direct effect on myopia development in guinea pigs. METHODS: Experiment 1: Guinea pigs wore occluders on the right eye for two weeks to induce form-deprivation myopia (FDM). Simultaneously they receive…

Repeat tick exposure elicits distinct immune responses in guinea pigs and mice.

Ticks deposit salivary proteins into the skin during a bite to mediate acquisition of a blood meal. Acquired resistance to tick bites has been demonstrated to prevent Borrelia burgdorferi sensu lato (s.l.) transmission. However, the mechanism of resistance, as well as the protective antigens, have remained elusive. To address these unknowns, we utilized a guinea pig model of tick resistance and a mouse model of permissiveness. Guinea pigs developed immunity after multiple Ixodes scapularis tick infestations, characterized by rapid tick detachment and impaired feeding. In comparison, mice toler…

Ngari virus (Orthobunyavirus, Peribunyaviridae) in ixodid ticks collected from cattle in Guinea.

Ngari virus is a mosquito-borne virus belonging to the genus Orthobunyavirus (Peribunyaviridae family). This virus is pathogenic to humans and causes severe illness. Ngari virus is present in several African countries, including Madagascar. Here, we report the detection of Ngari virus in ixodid ticks collected from cows in Guinea. A tick survey was conducted in March-November of 2018 in six regions of Guinea. The sample comprised 710 pools, with a total of 2067 ticks belonging to five species collected from 197 cows. At the initial stage, we screened a subsample of tick pools of vector-borne v…

New species of Cyrtodactylus (Squamata: Gekkonidae) from Karkar Island, Papua New Guinea.

We describe a new species of Cyrtodactylus from Karkar Island, an active volcano off the northern coast of New Guinea. It is a member of the C. sermowaiensis species group, which is defined by the absence of enlarged precloacal and femoral scales and the absence of precloacal/femoral pores in males. This species group includes two other species, one of which is widespread along the northern mountain ranges of New Guinea, and the other of which is restricted to Manus Island. The new species differs from each of these in its larger size; more-tuberculate dorsum; wide, even bands across the dorsu…