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Anti-ageing skin effects of Korean bamboo salt on SKH1 hairless mice.

Bamboo salt is generated by baking bamboo and sea salt and is used as a traditional food or medicine. The aim of this study was to investigate the anti-ageing skin effects of Korean bamboo salt and to compare the antioxidant, anti-ageing and anti-inflammatory effects of various salts, including purified salt, solar salt, bath solar salt, Masada solar salt, 1-time baked bamboo salt (1× bamboo salt), and 9-times baked bamboo salt (9× bamboo salt). Based on the content of mineral elements, pH, OH groups and redox potential amperometric analysis, the 9× bamboo salt showed the most antioxidant comp

GPR15LG regulates psoriasis-like inflammation by down-regulating inflammatory factors on keratinocytes.

Psoriasis is a common chronic inflammatory skin disease characterized by aberrant proliferation of keratinocytes and infiltration of immune cells. We previously found that GPR15LG protein is highly expressed in psoriasis lesional skin and it positively regulates psoriatic keratinocyte proliferation. Our data also showed that GPR15LG could regulate the activity of NF-κB pathway, which is associated with psoriatic inflammation. In the present study, we demonstrated that Gpr15lg (ortholog of GPR15LG) knockdown attenuated the severity of imiquimod (IMQ)-induced psoriasis-like inflammation in mice.

Enhancing transdermal delivery of retinyl acetate via transethosomes with emphasis on the impact of edge activators on penetration, safety, and efficacy.

Transethosomes are advanced lipid-based nanocarriers engineered to improve the transdermal delivery of therapeutic agents by combining phospholipids, ethanol, and edge activators (EAs), resulting in enhanced membrane fluidity, deformability, and skin penetration. This study aims to develop and optimize a novel transethosome-based delivery system for retinyl acetate (RA) to overcome its inherent limitations-including poor stability, low bioavailability, and skin irritation-thereby enhancing its therapeutic potential for dermatological and cosmetic applications. The transethosomes were prepared

Cannabinoid Receptor-2 Activation in Keratinocytes Contributes to Elevated Peripheral β-Endorphin Levels in Patients With Obstructive Jaundice.

BACKGROUND: Cholestatic diseases are often accompanied by elevated plasma levels of endogenous opioid peptides, but it is still unclear whether central or peripheral mechanisms are involved in this process, and little is known about the change of pain threshold in these patients. The purpose of this study was to determine the preoperative pain threshold, postoperative morphine consumption, and central and peripheral β-endorphin levels in patients with obstructive jaundice. This study also tests the hypothesis that activation of the cannabinoid receptor-2 (CB2R) in skin keratinocytes by endocan

Anti-adherent effects of Rhizophora apiculata bark and leaf extracts and computational prediction of the effects of its compound on β-tubulin interaction in Acanthamoeba triangularis genotype 4.

BACKGROUND AND AIM: Acanthamoeba, an opportunistic protozoan, exists widely in natural sources and can cause infections in humans and animals. The absence of effective monotherapy after the initial infection leads to chronic disease and recurrence. Tubulin protein is a vital target for design-targeted drug discovery. Anti-tubulin drugs are also used to treat Acanthamoeba infections, although resistance to these drugs has been observed. Therefore, it is necessary to identify a new targeted drug for Acanthamoeba infections. Therefore, this study aimed to assess the in vitro activity of ethanol e

A Case of Possible Concurrence of Dermatitis Herpetiformis and Linear Immunoglobulin A / Immunoglobulin G Bullous Dermatosis.

Dear Editor, Linear immunoglobulin (Ig) A bullous dermatosis (LABD), one subtype of subepidermal autoimmune bullous skin diseases (AIBDs), i … minal domains of BP180 region, BP230, purified laminin 332, and concentrated culture supernatant of HaCaT cells for LAD-1, were all negative. IgA ELISAs of tissue- and epidermal-transglutaminases were negative (1.92 AU/mL and 20.98 AU/mL, respectively; normal range <22.0 AU/mL). The patient was successfully treated with only topical corticosteroids with occas …

Low-level laser therapy prevents medication-related osteonecrosis of the jaw-like lesions via IL-1RA-mediated primary gingival wound healing.

BACKGROUND: Medication-related osteonecrosis of the jaw (MRONJ) is a serious debilitating disease caused by anti-resorption and anti-angiogenesis drugs, significantly affecting patients' quality of life. Recent studies suggested that primary gingival wound healing may effectively prevent the development of MRONJ. This study aimed to evaluate the effects of low-level light therapy (LLLT) on promoting gingival wound healing in extraction sockets of MRONJ-like mice and preventing the occurrence of MRONJ. Furthermore, we explored underlying mechanisms. METHODS: Mice were randomly divided into the

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… To detect the effects of Zol on HaCaT cells, we treated HaCaT cells with different concentrations of zoledronate, 1 μM, 5 μM, 10 μM, 25 μM, 50 μM, and found that 10 μM was the lowest concentration, at which HaCaT cells proliferation was inhibited at 24 h (P < 0.05, Fig. 4 A). … Fig. 4 LLLT promotes Zol-treated HaCaT cell migration. ( A ) CCK8 showed the effects of Zol at different concentrations on HaCaT cell proliferation at 24 h. ( B ) The migration assay of HaCaT cells treated with Zol and LLLT. …

Candidate Regulatory Relationship and Expression Correlation Between miR-33a-5p and ANK3 in Chronic Myeloid Leukemia.

Chronic myeloid leukemia (CML) is a type of bone marrow cancer characterized by the uncontrolled proliferation of myeloid cells. MicroRNAs (miRNAs) are small, non-coding RNA molecules that play a crucial role in the post-transcriptional regulation of gene expression. This study aims to examine the association between miR-33a-5p and Ankyrin 3 (ANK3) in CML and to investigate the regulatory mechanisms of miR-33a-5p in the progression of this disease. mRNA expression profiles were obtained from the GSE100026 dataset within the Gene Expression Omnibus (GEO) repository. Quantitative real-time polym

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… The expression levels of miR-33a-5p were evaluated in K562 and HaCaT cells using RT-qPCR. The results revealed a significantly higher expression of miR-33a-5p in K562 cells compared to HaCaT cells ( Figure 2 ). … The validation experiments were conducted using K562 and HaCaT cell lines, revealing that miR-33a-5p exhibited higher expression levels in K562 cells compared to HaCaT cells. Previous evidence supports a context-dependent role for miR-33a in cancer biology [ 14 ]. …

Post-Translational Modifications in Atopic Dermatitis: Current Research and Clinical Relevance.

Atopic dermatitis (AD) is a chronic and relapsing cutaneous disorder characterized by compromised immune system, excessive inflammation, and skin barrier disruption. Post-translational modifications (PTMs) are covalent and enzymatic modifications of proteins after their translation, which have been reported to play roles in inflammatory and allergic diseases. However, less attention has been paid to the effect of PTMs on AD. This review summarized the knowledge of six major classes (including phosphorylation, acetylation, ubiquitination, SUMOylation, glycosylation, o-glycosylation, and glycati

Trifuhalol A Suppresses Allergic Inflammation through Dual Inhibition of TAK1 and MK2 Mediated by IgE and IL-33.

The activation and degranulation of immune cells play a pivotal role in allergic inflammation, a pathological condition that includes anaphylaxis, pruritus, and allergic march-related diseases. In this study, trifuhalol A, a phlorotannin isolated from Agarum cribrosum, inhibited the degranulation of immune cells and the biosynthesis of IL-33 and IgE in differentiated B cells and keratinocytes, respectively. Additionally, trifuhalol A suppressed the IL-33 and IgE-mediated activation of RBL-2H3 cells through the regulation of the TAK1 and MK2 pathways. Hence, the effect of trifuhalol A on allerg

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… HaCaT cells were treated with 2 μM of LL-37 and irradiated with ultraviolet B (UVB) at 15 mJ/cm 2 after 24 h, followed by further incubation for 8 h to measure mRNA expression. … Detection of IL-33 in UVB-Irradiated and LL-37-Treated HaCaT cells The HaCaT cells were treated with 2 μM of LL-37 (Cayman Chemical, Ann Arbor, MI, USA) for 24 h prior to exposure to UVB (lamp Model G15T8E; 307 nm; Sankyo Denki, Japan) at an energy of 15 mJ/cm 2 , and trifuhalol A was administered ( …

Exploring the Therapeutic Potential of Ganoderma lucidum in Cancer.

Triterpenoids, such as ganoderic acid, and polysaccharides, including β-D-glucans, α-D-glucans, and α-D-mannans, are the main secondary metabolites of the medicinal fungus Ganoderma lucidum. There is evidence of the effects of ganoderic acid in hematological malignancies, whose mechanisms involve the stimulation of immune response, the macrophage-like differentiation, the activation of MAP-K pathway, an IL3-dependent cytotoxic action, the induction of cytoprotective autophagy, and the induction of apoptosis. In fact, this compound has been tested in twenty-six different human cancer cell types

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… Jurkat cells and the immortalized human epidermal HaCat cell line were used for the experiments; the cells were exposed to different concentrations of GLP (0 to 1000 mg/L) for 48 h. … On the other hand, the apoptosis rate in HaCat cells remained unchanged. …

Targeting the AMP-Activated Protein Kinase for Cancer Prevention and Therapy.

Despite the advances in biomedical research and clinical applications, cancer remains a leading cause of death worldwide. Given the limitations of conventional chemotherapeutics, including serious toxicities and reduced quality of life for patients, the development of safe and efficacious alternatives with known mechanism of action is much needed. Prevention of cancer through dietary intervention may hold promise and has been investigated extensively in the recent years. AMP-activated protein kinase (AMPK) is an energy sensor that plays a key role in the regulation of protein and lipid metabol

Curcumin: An age-old anti-inflammatory and anti-neoplastic agent.

Curcumin is a natural anti-inflammatory agent that has been used for treating medical conditions for many years. Several experimental and pharmacologic trials have demonstrated its efficacy in the role as an anti-inflammatory agent. Curcumin has been shown to be effective in treating chronic conditions like rheumatoid arthritis, inflammatory bowel disease, Alzheimer's and common malignancies like colon, stomach, lung, breast, and skin cancers. As treatments in medicine become more and more complex, the answer may be something simpler. This is a review article written with the objective to syst

Potential of Lycii Radicis Cortex as an Ameliorative Agent for Skeletal Muscle Atrophy.

Lycii Radicis Cortex (LRC) is a traditional medicine in East Asia with various beneficial effects, including antioxidant, anti-inflammatory, anti-tumor, anti-diabetic, and anti-depressant properties. However, its potential effects on skeletal muscle atrophy have not been studied. In this study, the protective effects of LRC extract (LRCE) on dexamethasone (DEX)-induced muscle atrophy were investigated in C2C12 myotubes and mice. We evaluated the effect of LRCE on improving muscle atrophy using a variety of methods, including immunofluorescence staining, quantitative polymerase chain reaction (

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… KB has been found to possess the antioxidant and anti-inflammatory properties of common SAs [ 34 , 35 ], and recent studies have demonstrated its ability to protect human keratinocyte HaCaT cells by inhibiting covalent modification by trans-2-nonenal [ 27 ]. … Kukoamine B from Lycii Radicis Cortex protects human keratinocyte HaCaT cells through covalent modification by trans-2-nonenal Plants 2022 12 163 10.3390/plants12010163 36616291 PMC9823295 28. Kim J.Y. Kim H.M. Kim J.H. Guo S. Lee D.H. Lim G.M. Kim W. Kim C.Y. …

Stem Cells as Potential Candidates for Psoriasis Cell-Replacement Therapy.

Recent years have seen considerable progress in explaining the mechanisms of the pathogenesis of psoriasis, with a significant role played in it by the hyper-reactivity of Th1 and Th17 cells, Treg function disorder, as well as complex relationships between immune cells, keratinocytes, and vascular endothelium. The effect of stem cells in the epidermis and stem cells on T cells has been identified and the dysfunction of various types of stem cells may be a prime cause of dysregulation of the inflammatory response in psoriasis. However, exploring these mechanisms in detail could provide a chance

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… Liu et al. confirmed the influence of MSC from lesions on proliferation and apoptosis of HaCaT-cells (aneuploid immortal keratinocyte cell line from adult human skin). … Research note mesenchymal stem cells from skin lesions of psoriasis patients promote proliferation and inhibit apoptosis of HaCaT cells. Genet. Mol. Res. 2015;14:17758–17767. doi: 10.4238/2015.December.21.49. 23. …

Marine Natural Products as Potent Anticancer Agents (2020-2024): Structural Diversity, SARs and Target Prediction.

In recent years, Marine Natural Products (MNPs) have emerged as a significant source for anticancer drug discovery, as many natural products can offer structural diversity, unique mechanisms of action, and relatively low toxicity. This article provides a systematic review of MNPs with reported anticancer activities from 2020 to 2024. These compounds are classified into seven major categories: terpenoids, alkaloids, sterols, polyketides, peptides and proteins, polysaccharides, and macrolides. For each category, we elaborate on the marine sources, structural identification, in vitro anticancer a

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… Compared with the positive control drug doxorubicin (IC 50 = 0.6 μM against A-375 and 22.1 μM against HaCaT), compound 49 exhibited weaker activity against A-375 cells (IC 50 = 2.8 μM) but stronger activity against HaCaT cells (IC 50 = 4.2 μM); compound 50 showed weaker activity against A-375 cells ( …

Апиген как потенциальный модулятор нейроинфламмации в многоразовом склерозе: обзор.

Множественный склероз (MS) — хроническое воспалительное заболевание центральной нервной системы (CNS), характеризующееся нейроинфламмацией и обезвоживанием. Несмотря на то, что в течение последнего десятилетия расширились возможности лечения MS, нынешняя терапия направлена главным образом на периферийные иммунные системы и часто связана со значительными побочными эффектами. Кроме того, эта терапия до сих пор не предлагает решений для последующего нейродегенерации, сопровождающего развитие болезни. Результаты исследований, проведенных с использованием клеточных и животных моделей и исследований

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… Anti-inflammatory, anti-proliferative and anti-psoriatic potential of apigenin in RAW 264.7 cells, HaCaT cells and psoriasis like dermatitis in BALB/c mice Life Sci. 2023 328 121909 10.1016/j.lfs.2023.121909 37414141 24. Zhang X. Wu C. … Effects of Apigenin on RBL-2H3, RAW264.7, and HaCaT Cells: Anti-Allergic, Anti-Inflammatory, and Skin-Protective Activities Int. J. Mol. Sci. 2020 21 4620 10.3390/ijms21134620 32610574 PMC7370139 88. Ishina I.A. Zakharova M.Y. Kurbatskaia I.N. Mamedov A.E. Belogurov A.A. Jr. Gabibov A.G. …

Pectin: A Bioactive Food Polysaccharide with Cancer Preventive Potential.

Pectin is an acidic heteropolysaccharide found in the cell walls and the primary and middle lamella of land plants. To be authorized as a food additive, industrial pectins must meet strict guidelines set forth by the Food and Agricultural Organization and must contain at least 65% polygalacturonic acid to achieve the E440 level. Fruit pectin derived from oranges or apples is commonly used in the food industry to gel or thicken foods and to stabilize acid-based milk beverages. It is a naturally occurring component and can be ingested by dietary consumption of fruit and vegetables. Preventing lo

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… A separate study explored pectin’s cytotoxic and anti-proliferative effects in HaCaT cells. MCP had an IC 50 of about 500 µg/mL, which was cytotoxic, and dose-dependent for both pectin and MCP [ 66 ]. … cell line) | Exhibited a stronger cytotoxic and anti-proliferative effect | [ 67 ] Brain cancer | Pectin extract (GW) | 10–400 μg mL −1 of GW | In vitro (U251-MG and T98 G human glioblastoma cell lines) | Induced cytotoxicity, ↑ cellular ROS levels | [ 68 ] Modified pectin | 50μL stock 3% solution | …

How to Treat Melanoma? The Current Status of Innovative Nanotechnological Strategies and the Role of Minimally Invasive Approaches like PTT and PDT.

Melanoma is the most aggressive type of skin cancer, the incidence and mortality of which are increasing worldwide. Its extensive degree of heterogeneity has limited its response to existing therapies. For many years the therapeutic strategies were limited to surgery, radiotherapy, and chemotherapy. Fortunately, advances in knowledge have allowed the development of new therapeutic strategies. Despite the undoubted progress, alternative therapies are still under research. In this context, nanotechnology is also positioned as a strong and promising tool to develop nanosystems that act as drug ca

Stem cell therapy as a potential treatment option for psoriasis.

Psoriasis is a chronic inflammatory dermatological disorder characterized by white scales and clearly demarcated erythematous plaques. The prevalence of psoriasis varies from country to country and can occur at any age, implying that ethnicity, environmental factors, and genetic background all play a role in its onset. According to the World Psoriasis Day Consortium, 125 million people globally and 2%-3% of the overall community have psoriasis. The introduction of biological treatments has revolutionized the treatment of moderate to severe psoriasis. These novel drugs, particularly those targe

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… Research Note Mesenchymal stem cells from skin lesions of psoriasis patients promote proliferation and inhibit apoptosis of HaCaT cells. Genet Mol Res. 2015;14:17758–17767. doi: 10.4238/2015.December.21.49. 16. Campanati A., Orciani M., Lazzarini R., Ganzetti G., Consales V., Sorgentoni G., et al. …