Результаты поиска

Activation of p53 in MDM2-overexpressing cells through phosphorylation.

Overexpressed MDM2 inactivates wild-type (wt) p53 in various human tumors. However, whether and how the wild-type p53 can be activated by an … another Burkitt's lymphoma cell line which carried mutant p53. Activation of p53 was accompanied by phosphorylation of p53 at Ser-15 and elevated p21 and MDM2, both of which were at least partly blocked by wortmannin, a kinase inhibitor against proteins with a PI3 kinase domain. Although MDM2 protein was rapidly cleaved and degraded after anticancer drug treatmen …

Adrenergic-mediated connexin43 phosphorylation in the ocular ciliary epithelium.

PURPOSE: Gap junctions provide metabolic cooperativity between the nonpigmented and pigmented cells of the ciliary epithelium. Connexin43 is the major protein of these junctions. To learn whether the phosphorylation state of this gap junction is sensitive to adrenergic mediators, we exposed isolated intact ciliary epithelia to agonists of the G-protein receptor-coupled system and analyzed the phosphorylation state of connexin43 by western blot. METHODS: The double layer of intact ciliary epithelia was isolated and exposed to isoproterenol, forskolin, and the tumor promoter 12-o-tetradecanoylp…

Trypanosoma cruzi-induced tyrosine phosphorylation in murine peritoneal macrophages.

Trypanosoma cruzi, the etiological agent of Chagas' disease, binds to and invades macrophages and other cells (fibroblasts, muscle cells) via a complicated set of interactions, but the changes induced by parasite-to-cell interactions are largely unknown. This report investigates the ability of T. cruzi to elicit a tyrosine kinase pathway in immature and mature resident murine peritoneal macrophages (MPM) that differ in their susceptibility to parasite infection. T. cruzi stimulated the phosphorylation of tyrosine residues in several endogenous substrates (proteins of 40-42, 53-56, 66, 75, 80, …

Inhibition of epidermal growth factor-induced phosphorylation by trifluoperazine.

We studied the effects of a series of drugs on A431, a cell line with well-characterized growth factor requirements and epidermal growth fac … effect on the binding, internalization, and processing of [125I]-EGF. In addition to the effects on phosphorylation, TFP inhibited cell growth both in the presence and absence of EGF. Morphologically, TFP blocked EGF-induced ruffling. TFP did not alter the EGF-stimulated phosphatidylinositol turnover. In an in vitro experiment using A431 cell membranes, TFP did n …

Фосфорилирование-дефосфорилирование ядерных белков во время инфекции (Q лихорадка).

Предложение о том, что выражение гена может регулироваться фосфорилацией нехистонных хроматиновых белков, было испытано путем изучения увеличения транскрипции в результате лихорадки Q. Некоторые виды деятельности, связанные с ядерным фосфопротеином печени и фосфатазной деятельностью, были изменены после того, как свиньи-морозки были заражены ожогом Коксиеллы. Протеины нонхистоунового хроматина увеличили активность фосфопротеиновых киназ и дифференцировали фосфорилаты. Добавление сперматозоида также стимулировало ядерные фосфопротеиновые киназы неинфекционной и зараженной печени. Заражение сопр

Совпадение в аннотации оригинала
The proposal that gene expression may be regulated by phosphorylation of nonhistone chromatin proteins was tested by studying increased transcription resulting from Q fever. Certain liver nuclear phosphoprotein kinase and phosphatase activities were altered after guinea pigs were infected with Coxiella burnetii. Nonhistone chromatin proteins had increased ph…

Dependence of platelet adenyl cyclase system on oxidative phosphorylation.

The radioactive adenosine 3',5'-monophosphate (cyclic AMP) level derived from 8-14C adenine in intact rabbit platelets decreased in the pres … results suggest that cyclic AMP synthesis in platelets is closely linked to mitochondrial oxidative phosphorylation.

LAT condensation gates PLCγ1 activation via bimodal LAT phosphorylation.

T cells can respond to even a single molecular binding event of antigen to a TCR. A key step in the TCR signaling pathway that definitively exhibits this single-molecule response is the initiation of calcium influx by activation of PLCγ1 in the LAT protein condensate. Here, we describe detailed kinetic measurements examining how protein condensation of LAT regulates activation of PLCγ1 using a reconstituted membrane system. The results reveal that membrane recruitment of PLCγ1 is tightly controlled by the LAT phosphorylation state, with no measurable independent recruitment to PIP2 or PIP3 lip…

Androgen rapidly activates phosphorylation signaling pathways in Sertoli cells.

Androgen-activated phosphorylation signaling pathways are of critical importance during spermatogenesis. The present study comprehensively analyzed androgen-activated phosphorylation signaling pathways in testicular Sertoli cells. Primary cultured mouse Sertoli cells were treated with testosterone for varying durations. Using tandem mass tag-labeled phosphoproteomics technology combined with Mfuzz clustering and functional enrichment analysis, key phosphorylation signaling pathways were identified. Mass spectrometry revealed 13,015 quantifiable phosphorylation sites, and Mfuzz clustering of ex…

SBK2-Driven NDUFV1 Phosphorylation and Translocation Limits Cardiac Hypertrophy.

BACKGROUND: Heart failure remains a major global health burden, driven largely by pathological cardiac hypertrophy. Mitochondrial dysfunctio … ased mitochondrial NDUFV1 promoted complex I activity, respiratory supercomplex assembly, oxidative phosphorylation, mitochondrial fusion, and redox homeostasis. Pharmacological inhibition of complex I or NDUFV1 silencing abolished SBK2-mediated protection. Moreover, a phospho-deficient NDUFV1 mutant (S251A) failed to rescue hypertrophic phenotypes in SBK2-defici …

Single-Oligomer Characterization of Tau Phosphorylation and Mechanical State.

Post-translational modifications have emerged as a key biomolecular process in the onset of neurodegenerative disorders, such as the hyperphosphorylation of tau protein in Alzheimer's disease (AD). High levels of phosphorylation are related to tau malfunction, self-assembly into amyloids forming neurofibrillary tangles in the brain, and cellular toxicity. These molecular processes are also reflected in human biofluids, allowing us to use tau phosphorylation as a biomarker of the disease onset and progression. However, it is not yet clear what structural changes the tau protein undergoes upon p…

CDK-dependent phosphorylation regulates PNKP function in DNA replication.

Okazaki fragment maturation (OFM) stands as a pivotal DNA metabolic process, crucial for genome integrity and cell viability. Dysregulation … kinase 1 and 2 (CDK1/2), which phosphorylates PNKP at multiple residues. Mutation analysis of these phosphorylation sites underscores the importance of CDK-mediated PNKP phosphorylation in DNA replication. Our findings collectively indicate a novel role for PNKP in facilitating Okazaki fragments joining, thus shedding light on its contribution to genome stability …

Acute Kaempferol Stimulation Induces AKT Phosphorylation in HepG2 Cells.

Type 2 diabetes mellitus (T2DM) stands as a prevalent global public health issue caused by deficiencies in the action of insulin and/or insu … lecular incorporation for both KM and KP. Specifically, KM exhibited the capability to increase AKT phosphorylation, a key kinase in insulin signaling, regardless of insulin receptor (IR) activation. Notably, KM showed an additional synergistic effect with insulin in activating AKT. In conclusion, our findings suggest the potential of KM as a promising compound f …

Mechanism of mitochondrial oxidative phosphorylation disorder in male infertility.

Male infertility has become a global concern, accounting for 20-70% of infertility. Dysfunctional spermatogenesis is the most common cause of male infertility; thus, treating abnormal spermatogenesis may improve male infertility and has attracted the attention of the medical community. Mitochondria are essential organelles that maintain cell homeostasis and normal physiological functions in various ways, such as mitochondrial oxidative phosphorylation (OXPHOS). Mitochondrial OXPHOS transmits electrons through the respiratory chain, synthesizes adenosine triphosphate (ATP), and produces reactiv…

Caveolin 3 suppresses phosphorylation-dependent activation of sarcolemmal nNOS.

Mutations of the caveolin 3 gene cause autosomal dominant limb-girdle muscular dystrophy (LGMD)1C. In mice, overexpression of mutant caveolin 3 leads to loss of caveolin 3 and results in myofiber hypotrophy in association with activation of neuronal nitric oxide synthase (nNOS) at the sarcolemma. Here, we show that caveolin 3 directly bound to nNOS and suppressed its phosphorylation-dependent activation at a specific residue, Ser1412 in the nicotinamide adenine dinucleotide phosphate (NADPH)-flavin adenine dinucleotide (FAD) module near the C-terminus of the reduction domain, in vitro. Constit…