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Comparison of the immunogenicity and protective efficacy of ACAM2000, MVA, and vectored subunit vaccines for Mpox in rhesus macaques.

The 2022-2023 mpox outbreak triggered vaccination efforts using smallpox vaccines that were approved for mpox, including modified vaccinia Ankara (MVA; JYNNEOS), which is a safer alternative to live replicating vaccinia virus (ACAM2000). Here, we compare the immunogenicity and protective efficacy of JYNNEOS by the subcutaneous or intradermal routes, ACAM2000 by the percutaneous route, and subunit Ad35 vector-based L1R/B5R or L1R/B5R/A27L/A33R vaccines by the intramuscular route in rhesus macaques. All vaccines provided robust protection against high-dose intravenous mpox virus challenge with t

The zoonotic poxviruses.

Animal poxviruses occasionally produce human skin disease often associated with systemic illness. Lesions are painful blisters and crusted eschars and are usually self-limiting and rarely fatal. Treatment is generally supportive.

Knowledge, attitude, and perception of Pakistani populations toward monkeypox: a cross-section study.

OBJECTIVE: The reappearance of monkeypox in non-endemic countries has preceded critical public health concerns. The public's adherence to preventative measures is influenced by their understanding of the infectious monkeypox virus (Mpox), attitude toward it, and perceptions of it. We conducted this study to evaluate the general population's knowledge, attitude, and perceptions of Mpox in Pakistan. METHODS: From August 15 to August 30, 2022, 3465 participants in this cross-sectional study provided information via an online survey. The survey has 37 questions divided into four categories: socio…

Применение усовершенствованного DdPCR для надежной количественной оценки MPXV в клинических условиях.

Вирус обезьяны (МПXV) представляет собой глобальную угрозу для здоровья людей. Цифровой ПЦР (ddPCR) обладает потенциалом в качестве точного диагностического инструмента для клинической микробиологии. Вместе с тем имеется ограниченная литература о применимости ДДПКВ в клинических условиях. В этом исследовании были проанализированы клинические характеристики пациентов с MPXV во время первоначальной вспышки в Китае в июне 2023 года, и был разработан оптимизированный метод DdPCR с удалением разбавления и/или ингибитора для повышения эффективности обнаружения MPXV. Восемьдесят два образца MPXV были

Совпадение в аннотации оригинала
UNLABELLED: Monkeypox virus (MPXV) poses a global health threat. Droplet digital PCR (ddPCR) holds potential as an accurate diagnostic tool for clinical microbiology. However, there is limited literature on the applicability of ddPCR in clinical settings. In this study, the clinical features of patients with MPXV during the initial outbreak in China in June …

One Health Investigation into Mpox and Pets, United States.

Monkeypox virus (MPXV) is zoonotic and capable of infecting many mammal species. However, whether common companion animals are susceptible to MPXV infection is unclear. During July 2022-March 2023, we collected animal and environmental swab samples within homes of confirmed human mpox case-patients and tested for MPXV and human DNA by PCR. We also used ELISA for orthopoxvirus antibody detection. Overall, 12% (22/191) of animal and 25% (14/56) of environmental swab samples from 4 households, including samples from 4 dogs and 1 cat, were positive for MPXV DNA, but we did not detect viable MPXV o…

Knowledge of human monkeypox viral infection among general practitioners: a cross-sectional study in Indonesia.

After the first, imported, laboratory-confirmed case of monkeypox in human was reported in Singapore on May 2019, countries in Asia started to strengthen disease surveillance systems. One challenge in preventing monkeypox is a lack of knowledge, particularly among healthcare workers. The aim of this study was to assess the knowledge of monkeypox among general practitioners (GPs) in Indonesia. A cross-sectional online survey was conducted. The survey collected participants' knowledge on a 21-item scale and explanatory variables. A two-step logistic regression analysis was employed to assess the

Управление и уход за африканскими общежитиями (Graphiurus kelleni).

Африканские общежития (Graphiurus spp.) представляют собой небольшие ночные грызуны, которые в настоящее время редко встречаются в лабораторных условиях. Их использование может возрасти, поскольку недавно было доказано, что они развивают инфекцию с вирусом обезьянопокс и могут оказаться ценной моделью животных для исследований инфекционных заболеваний. Поскольку африканское общежитие не доступно на рынке, для производства животных, необходимых для исследований, требуется обширная колония разведения. Изменения в муже, которые привели к увеличению производства потомства, были связаны с использов

Совпадение в аннотации оригинала
African dormice (Graphiurus spp.) are small nocturnal rodents that currently are uncommon in laboratory settings. Their use may increase as they have recently been shown to develop an infection with monkeypox virus and may prove to be a valuable animal model for infectious disease research. Because African dormice are not commercially available, an extensive…

Case report of a man with HIV presenting with malignant syphilis.

BACKGROUND: Malignant syphilis is a rare manifestation of secondary syphilis and is commonly associated with human immunodeficiency virus coinfection. It can present with multiorgan involvement, which poses a diagnostic dilemma to clinicians. METHODS: We report a case of a middle aged male who presented with near complete vision loss in his right eye with initial concern for fungal endophthalmitis due to his injecting drug use history. He concurrently had right cheek and forearm ulcerative plaques. RESULTS: He was diagnosed with disseminated syphilis following the punch biopsy of his right c

Recent Advances in Mpox Epidemic: Global Features and Vaccine Prevention Research.

Monkeypox (mpox) is a zoonotic disease (zoonose) caused by the monkeypox virus (MPXV). MPXV, a member of the Orthopoxviridae family, is categorized into two clades, Central Africa (I) and West Africa (II), each of which is further subdivided into subclades a and b. Clade I generally causes more serious illness and higher mortality rates, while Clade II results in milder illness. Historically, mpox epidemics were localized to specific regions and countries in Africa. Since 2022, the mpox epidemic, fueled by MPXV Clade IIb, has swiftly spread across various nations and regions, jeopardizing publ…

Challenges and Counteracting Strategies Including Optimum Health Service Practices for Frontline Nurses During the Mpox Outbreak and Futuristic Vision.

INTRODUCTION: Monkeypox (mpox) is an evolving infectious disease caused by the monkeypox virus (MPXV). On July 23, 2022, the WHO declared the recent mpox outbreaks a public health emergency of international concern (PHEIC), which terminated on May 11, 2023. As of July 11, 2023, 88,288 confirmed cases and 149 deaths have been reported from 112 countries and territories. Currently, mpox is not a PHEIC, as the outbreak and its impacts are nearly over. Nurses played significant roles during the mpox 2022 outbreak as frontline workers. PURPOSE: In light of the impending mpox global outbreak in 202…

Monkeypox: Treatment, Vaccination, and Prevention.

In regions where the disease is endemic, Monkeypox (MPV) transmission related to healthcare has been seen on numerous occasions. This diseas … serious problems for the general public's health. In addition to providing a broad overview of the Monkeypox Virus (MPXV), this study will detail the epidemiology, clinical hallmarks, assessment, and treatment of MPV sufferers.

A Case of Varicella Zoster and Mpox Coinfection in a Patient Living With HIV.

We present a case of recurrent, cutaneous mpox with coinfection of disseminated varicella zoster in an immunocompromised patient with poorly controlled HIV. This case demonstrates the importance of maintaining a high index of suspicion for mpox despite prior infection and vaccination, as suboptimal immune response is possible in immunocompromised patients, and also noting the potential for coinfection necessitating timely diagnosis and appropriate testing.

Enhanced detection of mpox virus in wastewater using a pre-amplification approach: A pilot study informing population-level monitoring of low-titer pathogens.

A recent outbreak of the mpox virus (MPXV) occurred in non-endemic regions of the world beginning in May 2022. Pathogen surveillance systems faced pressure to quickly establish response protocols, offering an opportunity to employ wastewater-based epidemiology (WBE) for population-level monitoring. The pilot study reported herein aimed to: (i) develop a reliable protocol for MPXV DNA detection in wastewater which would reduce false negative reporting, (ii) test this protocol on wastewater from various regions across the United States, and (iii) conduct a state of the science review of the curr