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Unraveling the role of PEGylation in the anti-aggregation stability of lipid nanoparticles.

PEGylation is a key strategy to increase the storage stability and prolong the in vivo circulation time of lipid-based drug delivery systems … work offers critical molecular insights and practical guidance for the rational design of PEGylated nanoparticles with optimal stability.

The Role of Glycolipids and their Toxicity in the Context of Nanomaterials and Nanoparticles: A Review of the Literature.

INTRODUCTION: Diseases triggered by glucose and lipid metabolic disorders, such as hyperglycemia and hyperlipidemia, have become a global he … es of literature searches, focusing on glycolipid effects and toxicity of nano-materials. RESULTS: Nanoparticles as drug carriers or nanoparticles enhance bioavailability and activity. Nano-material-based optical reporters aid in detecting lysosome lipid content, facilitating treatment and drug development for glucose and lipid metabolism disorders. Additional …

The multifunctional role of hydroxyapatite nanoparticles as an emerging tool in tumor therapy.

Hydroxyapatite nanoparticles (HANPs) are well-known nanomaterials for bone regeneration or repair. In recent years, HANPs have emerged as a potential tool in tumor therapy because of the numerous advantages the nanoparticles offer, including the diverse physicochemical properties, the selective anti-tumor effect, intrinsic immunomodulatory activity, ability to reverse of drug or immune tolerance, allowance of ion substation, good drug-loading capabilities, etc. Notably, the physicochemical properties of the particles, such as size and shape, significantly influence their anti-tumor efficacy. T…

The effect of alkyl termination on the optical and electronic properties of silicon nanoparticles.

In this study, we use a combination of (time-dependent) density functional theory and many-body perturbation theory methods to study the impact of alkyl termination on the optical and electronic properties of silicon nanoparticles (SiNPs), as well as the effect of increasing particle size. A comparative study of hydrogen and methyl-terminated SiNPs reveals that replacing hydrogen atoms with methyl groups results in a reduction of the fundamental gap, optical gap, and exciton binding energy. The effect of replacing hydrogen by methyl diminishes with the increasing size of the silicon core of th…

Endolysosome-targeted nanoparticle delivery of antiviral therapy for coronavirus infections.

SARS-CoV-2 can infect cells through endocytic uptake, a process that is targeted by inhibition of lysosomal proteases. However, clinically this approach to treat viral infections has afforded mixed results, with some studies detailing an oral regimen of hydroxychloroquine accompanied by significant off-target toxicities. We rationalized that an organelle-targeted approach will avoid toxicity while increasing the concentration of the drug at the target. Here, we describe a lysosome-targeted, mefloquine-loaded poly(glycerol monostearate-co-ε-caprolactone) nanoparticle (MFQ-NP) for pulmonary deli

Lipid nanoparticle screening in nonhuman primates with minimal loss of life.

Understanding how well delivery in mice predicts delivery in nonhuman primates (NHPs) could make lipid nanoparticle (LNP) discovery more efficient. Yet, few LNP-mRNA drug candidates are tested in NHPs, in part because the experiments require more animals than is considered ethical. Here, to minimize animal use, we create a pool of sterile barcoded LNPs that are frozen, aliquoted and administered when an end-of-life NHP-an animal that is independently scheduled for euthanasia due to spontaneous disease-becomes available. We then administer this pool of 45 LNP-aVHH mRNAs with different chemistri

Advances in anthocyanin nanoparticle delivery systems in anti-inflammatory therapies.

Chronic inflammation, a major global health burden, is a significant contributor to various diseases, including liver and kidney failure, in … comprehensive reviews has yet systematically examined the anti-inflammatory activity of ACN-loaded nanoparticles (NPs). In this review, we provide an in-depth summary of the preparation techniques, physicochemical properties, and functional characteristics of ACNs-loaded NPs based on polysaccharides, proteins, lipids, and metal NPs, and critically evaluate the …

An innovative infection method for the accumulation of viral nanoparticles in Nicotiana benthamiana.

Tomato Bushy Stunt Virus (TBSV) naturally infects tomato plants, although it can also infect other plant species, such as Nicotiana benthamiana, a known model system in plant molecular farming. In the presented work, a novel system for TBSV infection of Nicotiana benthamiana plants, designed to produce nanomaterials, was developed and optimized based on a simple foliar spray, without the use of surfactants. Up to now, the standard procedures for the viral infection have been syringe or vacuum infiltration, which are a time-consuming manual procedure or requiring expensive machinery, respective

Preparation and Immunogenicity Evaluation of a Ferritin-Based GnRH Nanoparticle Vaccine.

Objectives: Research on the immunocastration vaccine is of great significance for animal management. In this study, the gonadotropin-releasing hormone (GnRH) ferritin nanoparticle vaccine was constructed using Spy Catcher-Spy Tag (SC-ST) as a delivery system; Methods: The Spy Catcher was constructed to fuse with the expression vector pET-30a-SF of ferritin nanoparticles. Two polypeptides, STG1: Spy Tag-GnRH I-PADRE and STG2: Spy Tag-GnRH I-GnRH II, coupled to SF in vitro to form two nanoparticles, were designed and synthesized to detect castration effects in mice. We mixed them with the adjuva…

[Research progress on lipid nanoparticle messenger RNA delivery system].

Messenger RNA (mRNA) therapeutics involve delivering in vitro transcribed mRNA into specific cells to produce target proteins for the treatment or prevention of diseases. However, the development of mRNA therapeutics relies largely on mRNA delivery systems. Lipid nanoparticles (LNPs) represent the most widely used mRNA carriers in clinical applications. Composed of ionizable lipids, zwitterionic phospholipids, cholesterol, and polyethylene glycol-lipids, LNPs can address critical challenges in mRNA drug development, such as poor in vivo stability and the difficulty in crossing biological barri…

Synergistic Effect of Copper Nanoparticles and Paromomycin in the Treatment of Cutaneous Leishmaniasis.

The interest in nanotechnology applications in medicine, particularly for combating microbial infections, has surged in recent years. This study investigated the in vitro and in vivo antileishmanial effects of copper nanoparticles (CuNPs) that were green synthesised using Capparis spinosa fruit extract, both on their own and in conjunction with paromomycin (PM). CuNPs were synthesised from a methanolic extract of C. spinosa. We assessed the in vitro antileishmanial activity of CuNPs (10-200 μg/mL) as well as the same concentrations of CuNPs (10-200 μg/mL) combined with PM (10-200 μg/mL), targe…

Current advancements in nanoparticles for vaccines and drug delivery for the treatment of tuberculosis.

Since the early centuries until the present, tuberculosis has been a global illness with no cure. However, the sickness remains dormant in t … This article addresses tuberculosis infection diagnosis, pathophysiology, immunology, and advanced nanoparticles to deliver TB vaccines and anti-TB drugs. The challenges and prospects for the future in creating secure and functional nanoparticles for tuberculosis treatment and diagnosis for the good health and well-being of the patient are also discussed.

Дистанционная загрузка автоантигена в наночастицы PLGA для лечения множественного склероза.

Аутоиммунные заболевания, такие, как множественный склероз (МС), затрагивают миллионы людей во всем мире и становятся все более распространенными. Нынешние терапевтические стратегии либо полностью подавляют иммунную функцию, либо обеспечивают лишь скромную эффективность. В последнее время усилия в области исследований сместили акцент на антигенные методы лечения, с тем чтобы содействовать иммунной терпимости и избегать подрыва общей иммунной функции. Здесь мы показываем применение нового метода удалённой нагрузки с использованием наночастиц из наночастицы (PLGA NPs) для загрузки mielin oligode

Совпадение в аннотации оригинала
Autoimmune diseases like multiple sclerosis (MS) affect millions of people worldwide and have been growing in prevalence. Current therapeuti … e show the application of a novel aqueous remote loading method using poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) to load myelin oligodendrocyte glycoprotein (MOG) peptide at high loading and encapsulation efficiency. MOG is a target of autoreactive T cells in MS. These NPs (750 ± 200 nm and - 16.7 ± 0.4 mV zeta potential) slowly and continuously rel …

Липидная наночастица (ЛНП) является посредником при родах MRNA в нейродегенеративных заболеваниях.

Нейродегенеративные заболевания (НДД) характеризуются постепенной потерей нейронов и ухудшением функций клеток. Посланник РНК (МРНК) стал перспективной терапией для лечения НДД, поскольку он может кодировать недостающие или нефункционирующие белки и антивоспалительные цитокины или нейрозащитные белки, чтобы остановить прогрессию этих заболеваний. Вместе с тем эффективная доставка МРНК в центральную нервную систему (КНС) по-прежнему является серьезной проблемой из-за ограниченного проникновения кровеносного барьера (ББД). Липидные наночастицы (LNPs) предлагают эффективное решение путем инкапсул

Совпадение в аннотации оригинала
Neurodegenerative diseases (NDD) are characterized by the progressive loss of neurons and the impairment of cellular functions. Messenger RN … ains a significant challenge due to the limited penetration of the blood-brain barrier (BBB). Lipid nanoparticles (LNPs) offer an efficient solution by encapsulating and protecting mRNA, facilitating transfection and intracellular delivery. This review discusses the pathophysiological mechanisms of neurological disorders, including Parkinson's disease (PD), Alz …

A Multifunctionalized Potyvirus-Derived Nanoparticle That Targets and Internalizes into Cancer Cells.

Plant viral nanoparticles (VNPs) are attractive to nanomedicine researchers because of their safety, ease of production, resistance, and straightforward functionalization. In this paper, we developed and successfully purified a VNP derived from turnip mosaic virus (TuMV), a well-known plant pathogen, that exhibits a high affinity for immunoglobulins G (IgG) thanks to its functionalization with the Z domain of staphylococcal Protein A via gene fusion. We selected cetuximab as a model IgG to demonstrate the versatility of this novel TuMV VNP by developing a fluorescent nanoplatform to mark tumor…

Biodistribution of Polymeric Nanoparticles following in utero Delivery to a Nonhuman Primate.

INTRODUCTION: Monogenic diseases can be diagnosed before birth. Systemic fetal administration of nanoparticles (NPs) grants therapeutic access to developing stem cell populations impacted by these classes of disease. Delivery of editing reagents in these NPs administered before birth has yielded encouraging results in preclinical mouse models of monogenic diseases. METHODS: To translate this strategy clinically, the safety and efficacy of this strategy in larger animals will be necessary. We performed a pilot biodistribution study in 3 fetal nonhuman primates (NHPs) in mid-gestation examining…

The mitochondria-targeted Kaempferol nanoparticle ameliorates severe acute pancreatitis.

Kaempferol (KA), an natural antioxidant of traditional Chinese medicine (TCM), is extensively used as the primary treatment for inflammatory digestive diseases with impaired redox homeostasis. Severe acute pancreatitis (SAP) was exacerbated by mitochondrial dysfunction and abundant ROS, which highlights the role of antioxidants in targeting mitochondrial function. However, low bioavailability and high dosage of KA leading to unavoidable side effects limits clinical transformation. The mechanisms of KA with poor bioavailability largely unexplored, hindering development of the efficient strategi

Cocktail Approach with Polyserotonin Nanoparticles and Peptides for Treatment of Streptococcus mutans.

Dental plaque, formed by a Streptococcus mutans biofilm, is a major contributor to cavity formation. While antimicrobial strategies exist, the growing risk of antibiotic resistance necessitates alternative therapeutic solutions. Polyserotonin nanoparticles (PSeNPs), recently recognized for their photothermal property and promising biomedical applications, open up a new avenue for antimicrobial use. Here, we introduced a UV-initiated synthetic route for PSeNPs with improved yield. Using these PSeNPs, a cocktail treatment to reduce the viability of this cavity-causing bacteria was developed. Thi…

Rethinking the application of nanoparticles in women's reproductive health and assisted reproduction.

Nanoparticles and nanotechnology may present opportunities to revolutionize the prevention, treatment and diagnosis of a range of reproductive health conditions in women. These technologies are also used to improve outcomes of assisted reproductive technology. We highlight a range of these potential clinical uses of nanoparticles for polycystic ovary syndrome, endometriosis, uterine fibroids and sexually transmitted infections, considering in vitro and in vivo studies along with clinical trials. In addition, we discuss applications of nanoparticles in assisted reproductive technology, includin…

Efficacy and Safety of Dual Paclitaxel and Sirolimus Nanoparticle-Coated Balloon.

We evaluated a novel dual active pharmaceutical ingredient (API) drug-coated balloon (DCB), which consists of a coating of nanoparticles encapsulating low-dose paclitaxel (PTX) in combination with sirolimus in a synergistic ratio. Compared to the PTX DCB, the dual API DCB demonstrated similar inhibition of cell proliferation in vitro but at a significantly lower total drug dose (over 13 times lower than sirolimus nanoparticles). Animal experiments demonstrated that the dual API DCB is more effective in inhibiting intimal cell proliferation with insignificant downstream embolic effects and myoc…