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Microbiota transplantation and administration of live biotherapeutic products for the treatment of dysbiosis-associated diseases.

INTRODUCTION: The microbiota composition in humans varies according to the anatomical site and is crucial for maintaining homeostasis and an overall healthy state. Several gastrointestinal, vaginal, respiratory, and skin diseases are associated with dysbiosis. Alternative therapies such as microbiota transplantation can help restore microbiota normal composition and can be implemented to treat clinically relevant diseases. AREAS COVERED: Current microbiota transplantation therapies conducted in clinical trials were included in this review (after searching on MEDLINE database from years 2017 t

Diagnostic Performance of Artificial Intelligence in Predicting Malignant Upgrade of B3 Breast Lesions: Systematic Review and Meta-Analysis.

Background/Objectives: High-risk (B3) breast lesions are a heterogeneous group with uncertain malignant potential. Methods: We systematically reviewed and meta-analyzed the ability of artificial-intelligence (AI) models to predict malignant upgrades (a ductal carcinoma in situ or an invasive carcinoma) after biopsy. A comprehensive search of medical and engineering databases through 27 July 2025 identified retrospective studies that developed or validated AI models for upgrade prediction in cohorts with ≥20 B3 lesions and confirmed outcomes at surgical excision or after ≥24 months of follow-up

A Comparative Review on the Production of Factor VIII in Human and Non-human Hosts.

Hemophilia A (HA) is an inherited condition that is characterized by a lack of coagulation factor VIII (FVIII), which is needed for blood clotting. To produce Recombinant Factor VIII (rFVIII) for treatment, innovative methods are required. This study presents a thorough examination of the genetic engineering and biotechnological methods that are essential for the production of this complex process. Multiple host cells, such as animal, microbial, and human cell lines, are examined. Cultivating genetically modified cells enables the production of rFVIII, with further changes after protein synthe

Pyromania/ Firesetters and Attention Deficit Hyperactivity Disorder in children and adolescents: a systematic review.

BACKGROUND: The following article aims to explore the relationship between Pyromania/Firesetters and Attention Deficit Hyperactivity Disorder (ADHD) in childhood and adolescence. METHOD: A systematic literature review was carried out using search engines such as PubMed, PsycINFO, Cochrane, and Sicelo, to find relevant articles. Inclusion criteria included studies that related ADHD and Pyromania/Firesetters, while exclusion criteria included articles not related to the researched variables, articles from other systematic reviews, and articles not related to childhood and/or adolescence. RESUL…

Adoptive transfer of NK cells engineered with a CD5-based chimeric antigen receptor (SRCD5CAR) to treat invasive fungal infections.

The treatment of invasive fungal infections (IFIs) poses a major health challenge due to their severity, the scarcity and toxicity of current antifungal drugs, and the emergence of drug-resistant strains, thus forcing the search for novel strategies. Approaches involving adoptive transfer of immune cells harboring chimeric antigen receptors (CARs) are gaining momentum in several clinical settings. Based on the fungal β-glucan-binding properties of the lymphocyte scavenger receptor CD5, we assayed NK and T cells engineered with a CD5-based second-generation CAR (SRCD5CAR) for the treatment of I

Machine Learning for Multi-Omics Characterization of Blood Cancers: A Systematic Review.

Artificial Intelligence and machine learning are increasingly used to interrogate complex biological data. This systematic review evaluates their application to multi-omics for the molecular characterization of hematological malignancies, an area with unmet clinical need. We searched PubMed, Embase, Institute of Electrical and Electronics Engineers Xplore, and Web of Science from January 2015 to December 2024. Two reviewers screened records, extracted data, and used a modified appraisal emphasizing explainability, performance, reproducibility, and ethics. From 2847 records, 89 studies met incl

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… Relevant articles were sourced using the search engines identified above, then further deeper searches were conducted using Claude.AI. Claude.AI was also used to obtain the metadata for the publications selected. 2.5. … Acknowledgments This systematic review utilized Claude.AI to identify study references as an additional step to the search engine methods stated in the text. Further evaluation of the studies around the stated criteria and metadata selected studies was also conducted using Claude.AI. …

3D printed skin dressings manufactured with spongin-like collagen from marine sponges: physicochemical properties andin vitrobiological analysis.

The search for innovative materials for manufacturing skin dressings is constant and high demand. In this context, the present study investigated the effects of a 3D printed skin dressing made of spongin-like collagen (SC) extract from marine sponge (Chondrilla caribensis), used in 3 concentrations of SC and alginate (C1, C2, C3). For this proposal, the physicochemical, morphological andin vitrobiological results were investigated. The results demonstrated that, after immersion, C2 presented a higher mass loss and C3 present a higher pH in experimental periods. Also, a higher porosity was obse

Mild hypothermia: Insights and implications for productivity in mammalian and insect cell cultures.

Mammalian cells are the preferred expression system for obtaining recombinant proteins (r-proteins) due to their ability to generate human-like glycosylation patterns. However, their slow growth and lower productivity compared to prokaryotic host cells, coupled with the rising demand for complex therapeutic proteins in the biopharmaceutical market, have driven the search for alternatives to boost productivity. In this context, mild hypothermia (MH) has emerged as a valuable tool for enhancing both the viability and productivity of r-proteins in mammalian as well as some insect-derived cell lin

Multi-strategy improved gazelle optimization algorithm for numerical optimization and UAV path planning.

The Gazelle Optimization Algorithm (GOA) is a recently proposed and widely recognized metaheuristic algorithm. However, it suffers from slow convergence, low precision, and a tendency to fall into local optima when addressing practical problems. To address these limitations, we propose a Multi-Strategy Improved Gazelle Optimization Algorithm (MIGOA). Key enhancements include population initialization based on an optimal point set, a tangent flight search strategy, an adaptive step size factor, and novel exploration strategies. These improvements collectively enhance GOA's exploration capabilit

A Proteomics Outlook on the Molecular Effectors of CAR-T Cell Therapy in Cancer Management.

In the field of cell and immunotherapy, chimeric antigen receptor T-cell (CAR-T cell) therapy is the state-of-the-art therapy. It utilizes genetically engineered T cells expressing receptors against specific tumor cell targets such as CD-19 to induce cytotoxicity in and kill malignant cells. CAR-T cell therapy has demonstrated tremendous success in hematological cancers and promising results in solid tumors. However, CAR-T cell therapy has some limitations, such as causing cytokine release syndrome and neurologic abnormalities, leading to loss of target, and, most importantly, its high cost. P

Bioterrorism potential of haemorrhagic fever viruses - occupational and environmental Implications of filoviruses and arenaviruses.

INTRODUCTION AND OBJECTIVE: Viral haemorrhagic fevers (VHFs), caused by filoviruses (e.g., Ebola virus, Marburg virus) and arenaviruses (e.g., Lassa virus, Machupo virus), represent a significant bioterrorism threat due to their zoonotic origins, high mortality rates, and severe clinical presentations. This review examines the potential use of VHFs as biological weapons, their zoonotic transmission dynamics, and implications for rural and agricultural health. REVIEW METHODS: A comprehensive review was conducted using electronic databases, including PubMed and Scopus, focusing on studies addre

Are Buccal Fat Pad-Derived Stem Cells Effective in Adults for Maxillary Bone Regeneration? A Systematic Review.

Critical-size bone defects in the maxillofacial region remain a major challenge. Although autografts are widely used, they are limited by donor-site morbidity and graft availability. Tissue engineering with mesenchymal stem cells, particularly buccal fat pad-derived stem cells (BFPSCs), offers a promising alternative due to their accessibility and osteogenic potential. This systematic review assesses clinical evidence on the use of BFPSCs for regenerating oro-maxillofacial bone defects in adults. A systematic search was conducted in PubMed/MEDLINE, EMBASE, and Cochrane databases for studies fr

Mediators of Periodontitis complementing the development of Neural Disorders.

As a common oral health concern, periodontitis has been a source of attention for the global health community because of its linkage with systemic and neurological diseases. The purpose of the present study is to reveal the mediating role of specific cytokines, neuropeptides, and pathogens in the association of chronic periodontitis and neural disorders. To find the related literature different search engines namely PMC, Science Direct, PubMed, Research Gate, and Google Scholar were explored for a study period of five months from October 2022 to February 2023. This review offers a summary of t…

Genome Mining Leads to the Identification of a Stable and Promiscuous Baeyer-Villiger Monooxygenase from a Thermophilic Microorganism.

Baeyer-Villiger monooxygenases (BVMOs) are NAD(P)H-dependent flavoproteins that convert ketones to esters and lactones. While these enzymes offer an appealing alternative to traditional Baeyer-Villiger oxidations, these proteins tend to be either too unstable or exhibit too narrow of a substrate scope for implementation as industrial biocatalysts. Here, sequence similarity networks were used to search for novel BVMOs that are both stable and promiscuous. Our genome mining led to the identification of an enzyme from Chloroflexota bacterium (strain G233) dubbed ssnBVMO that exhibits i) the highe

Mechanistic Evaluation of miRNAs and Their Targeted Genes in the Pathogenesis and Therapeutics of Parkinson's Disease.

MicroRNA (miRNA) are usually 18-25 nucleotides long non-coding RNA targeting post-transcriptional regulation of genes involved in various bi … tudy, a systematic evaluation of research articles from year 2017 to 2022 was performed on multiple search engines, and lists of miRNA being dysregulated in PD in different body components were generated. This study highlighted miR-7, miR-124, miR-29 family, and miR-425, showing altered expression levels during PD's progression, further regulating the expressio …

Aripiprazole and its adverse effects in the form of impulsive-compulsive behaviors: A systematic review of case reports.

Aripiprazole is an efficacious treatment for both the positive and negative symptoms of schizophrenia and is also commonly used as a mood st … rts published in this regard. After screening more than 6000 titles and abstracts in ten scientific search engines, we found 35 related records comprising 59 cases. The majority of cases (n = 42, 71.18%) were male, the mean age was 33.83 years (± 13.40), and the average daily dose of aripiprazole was 11.63 mg (± 6.94). The results of our review showed that the …

From classical approaches to new developments in genetic engineering of live attenuated vaccine against cutaneous leishmaniasis: potential and immunization.

Despite the development of a vaccine against cutaneous leishmaniasis in preclinical and clinical studies, we still do not have a safe and effective vaccine for human use. Given this situation, the search for a new prophylactic alternative to control leishmaniasis should be a global priority. A first-generation vaccine strategy-leishmanization, in which live Leishmania major parasites are inoculated into the skin to protect against reinfection, is taking advantage of this situation. Live attenuated Leishmania vaccine candidates are promising alternatives due to their robust protective immune re

The Link Between Apical Periodontitis and Gastrointestinal Diseases-A Systematic Review.

INTRODUCTION: This systematic review aimed to examine a potential link between apical periodontitis (AP) and gastrointestinal diseases (GIDs). METHODS: The protocol of the review has been registered in PROSPERO (CRD42022330771). The following engines were used with the aim of searching for relevant literature: PubMed, Web of Science, Scopus, Cochrane Central Register of Controlled Trials, and grey literature, from inception to May 2022. There were no language restrictions included. Study selection, data collection, and synthesis have been performed by 2 independent reviewers. For the purpose

Exploring female medical students' knowledge, attitudes, practices, and perceptions related to breast cancer screening: a scoping review.

Early detection measures for breast cancer, such as breast self-exams, clinical breast exams, and mammography, have considerable benefits in … ing Reviews (PRISMA-ScR) template. Our analysis included 43 articles from Google Scholar and PubMed search engines, focusing on female medical students. Our results showed that most female medical students had a satisfactory level of knowledge about the most common signs, symptoms, and early detection methods of breast cancer. Generally, their attitude and perc …

Osteosarcopenia and Pain: Do We Have a Way Out?

Osteosarcopenia (OSP) is a geriatric syndrome characterized by the coexistence of osteoporosis and sarcopenia and associated with an increas … actors. Based on this evidence, we conducted a narrative review using the PubMed and Google Scholar search engines to summarize the current knowledge on the molecular mechanisms involved in the pain development in OSP and the potential countermeasures to be taken. The lack of studies addressing this topic highlights the need to conduct new research into the res …