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Identification of genes potentially involved in LMO2-induced leukemogenesis.

The most common translocations in childhood T cell acute lymphoblastic leukemias involve the LMO2 locus on chromosome 11p13 and cause ectopic expression of the LMO2 gene in thymocytes. Transgenic mice with enforced expression of LMO2 in their thymocytes develop T cell leukemias thus demonstrating the role of LMO2 in leukemogenesis. The physiologic and leukemogenic functions of LMO2 are mediated through its transcriptional regulatory activities, but the identity of the target genes is completely unknown. In this report, we have used cDNA representational difference analysis (cDNA-RDA) to identi

Complications and outcomes after three-column osteotomy: a 5-year follow-up.

BACKGROUND: Three-column spinal osteotomies (3-CO) are powerful techniques used to correct spinal deformity. These surgeries are associated with an elevated risk of complications. While outcomes have been reported in the literature with 2 years follow-up, longer-term outcomes of three-column osteotomies remain understudied. OBJECTIVES: This study aims to examine patient reported outcomes and complications for three-column osteotomies at 5 years. STUDY DESIGN: Retrospective review of a prospectively collected spinal deformity cases database. PATIENT SAMPLE: All consecutive adult patients at

Utility of the anesthetic test dose to avoid catastrophic injury during cervical transforaminal epidural injections.

BACKGROUND CONTEXT: Reports of serious complications from cervical transforaminal epidural corticosteroid injections often consider accidental intra-arterial injection the most likely mechanism of injury. As a result, many physicians have instituted methods to prevent intravascular injections. Routine use of the anesthetic test dose is one such method. The utility of the anesthetic test dose in this function has not been characterized in the current literature. PURPOSE: The aim of this study was to determine the utility of injecting an anesthetic test dose before cervical transforaminal epidu

The Transfemoral and Transhumeral OPRA (Osseoanchored Prostheses for the Rehabilitation of Amputees) Osseointegration Technique.

BACKGROUND: The transfemoral or transhumeral Osseoanchored Prostheses for the Rehabilitation of Amputees (OPRA) osseointegration technique is indicated in patients with transfemoral or transhumeral amputations who have difficulty with use of a conventional socket and traditional prosthesis. Augmentations to the procedure, such as targeted muscle reinnervation (TMR) and/or a regenerative peripheral nerve interface (RPNI), serve to enhance function (in the case of TMR) and/or improve residual-limb neuropathic pain (in the case of combined TMR and RPNI) in the same patient population. DESCRIPTIO

Cochlear Place Specificity of the Auditory Brainstem Response to Narrowband Chirp Versus 2-1-2 Stimuli: High-Pass Noise/Derived Responses.

OBJECTIVES: In recent years, many researchers have recommended using narrowband chirp (NBchirp) stimuli for auditory brainstem response (ABR) audiometry instead of more-standard 2-1-2 cycles linear-gated tones, primarily because NBchirps often result in larger ABR wave V amplitudes. However, the acoustic frequency spectra of currently recommended NBchirps are wider than those for 2-1-2 tones, and it is currently not known whether ABRs to these NBchirps have similar (or poorer) cochlear place specificity compared with 2-1-2 tones. The present study used the high-pass noise/derived response tech

Minimally invasive video-assisted parathyroidectomy versus open minimally invasive parathyroidectomy for a solitary parathyroid adenoma: a prospective, randomized, blinded trial.

BACKGROUND: A variety of minimally invasive parathyroidectomy (MIP) techniques have been currently introduced to surgical management of primary hyperparathyroidism (pHPT) caused by a solitary parathyroid adenoma. This study aimed at comparing the video-assisted MIP (MIVAP) and open MIP (OMIP) in a prospective, randomized, blinded trial. MATERIALS AND METHODS: Among 84 consecutive pHPT patients referred for surgery, 60 individuals with concordant localization of parathyroid adenoma on ultrasound and subtraction Tc99m-MIBI scintigraphy were found eligible for MIP under general anesthesia and we

Clinical Efficacy of Pulsed Radiofrequency Combined with Intravenous Lidocaine Infusion in the Treatment of Subacute Herpes Zoster Neuralgia.

OBJECTIVE: Under the guidance of a digital subtraction angiography (DSA) machine, via fluoroscopic imaging techniques, patients diagnosed with herpes zoster neuralgia at the subacute stage, where self-reported pain lasts between 30 and 90 days, were treated with nerve pulsed radiofrequency surgery combined with intravenous lidocaine infusion or saline infusion as control. This study explores the clinical efficacy, safety, and clinical value of the combined treatment compared with nerve pulsed radiofrequency surgery alone. METHODS: In this study, 72 patients diagnosed with herpes zoster neural

Bioengineered vascular access maintains structural integrity in response to arteriovenous flow and repeated needle puncture.

OBJECTIVE: Tissue-engineered blood vessels (TEBV) have been proposed as an alternative to prosthetic grafts for dialysis access. However, arteriovenous (AV) grafts must withstand extreme flow rates and frequent needle trauma. In a proof-of-concept study, we sought to determine whether scaffold-based TEBV could withstand the hemodynamic and mechanical challenges of chronic dialysis access. METHODS: TEBV were constructed using decellularized arterial scaffolds seeded with autologous ovine endothelial cells (EC) derived from circulating endothelial progenitor cells (EPC) using a novel high-affin

Increased expression of protein kinase Calpha, interleukin-1alpha, and RhoA guanosine 5'-triphosphatase in osteoblasts expressing the Ser252Trp fibroblast growth factor 2 receptor Apert mutation: identification by analysis of complementary DNA microarray.

Apert (Ap) syndrome is a craniofacial malformation characterized by premature fusion of cranial sutures (craniosynostosis). We previously showed that the Ser252Trp fibroblast growth factor receptor 2 (FGFR-2) mutation in Ap syndrome increases osteoblast differentiation and subperiosteal bone matrix formation, leading to premature calvaria ossification. In this study, we used the emerging technology of complementary DNA (cDNA) microarray to identify genes that are involved in osteoblast abnormalities induced by the Ser252Trp FGFR-2 mutation. To identify the signaling pathways involved in this s

Pigmented villonodular synovitis (PVNS) of the knee joint: magnetic resonance imaging (MRI) using standard and dynamic paramagnetic contrast media. Report of 52 cases surgically and histologically controlled.

PURPOSE: Pigmented villonodular synovitis (PVNS) is a rare proliferative disorder of the synovial membrane, exhibiting benign behaviour from a biological point of view. This kind of synovial hyperplasia leads to the formation of villi and nodules characterized by deposit of intracellular haemosiderin. It primarily involves young adults, the peak age being between the second and fourth decade of life. It may appear either in a diffuse or a localized (nodular) form. The joint most affected is the knee and diffuse PVNS is the most common form. Diagnostic imaging techniques, particularly MRI, allo

Posterior vertebral column resection: Exploring practical uses in clinical settings.

BACKGROUND: The purpose of this study was to present our experience in patients who had been treated with posterior vertebral column resection (PVCR) for various spinal deformities. METHODS: Thirty-seven patients who performed PVCR between 2015 and 2018 were evaluated retrospectively. The mean follow-up period was 24 months (range: 12-50 months). The demographic data of the patients, mean blood loss, amount of blood replacement, duration of operation, intensive care and hospitalization period, PVCR level, instrumentation level, amount of preoperative curvature, amount of postoperative curvatu

Трабковая аттенуляция L1 у взрослых пациентов с множественной миеломой: исследование с целью наблюдения за изображением низкодозволенной КТ.

Цель этого исследования заключалась в оценке воздействия трабекулярного сглаживания позвоночного тела L1 в низкодозированной КТ у взрослых пациентов с множественной миеломой (ММ), парящей множественной миеломой (ШМ) и моноклональной гаммопатией неопределенного значения (МГУС). МАТЕРИАЛЫ И МЕТОДЫ. 22 пациентов с MGS и 51 последовательные пациенты с новым диагнозом MM (SMM, n = 21; симптоматическая MM, n = 36). Сканирование КТ проводилось с использованием 128-slice Сканер КТ (Somatom go.Top, Siemens, Мюнхен, Германия). Сканирование всего тела с низкой дозой проводилось в одно время для каждого п

Одинокий квантовый Sodium MRI на 3T для разделения сигналов Sodium и Mono и B-T2.

Из-за десятикратной разницы в концентрации натрия на мембранах, которая активно поддерживается насосом с натрием-потассием (Na+-K+), клеточный ионный баланс очень чувствительн. Разрывы в этом насосе или мембранной целостности, наблюдаемые в таких неврологических расстройствах, как эпилепсия, множественный склероз, биполярное заболевание и легкая травма головного мозга, приводят к увеличению внутриклеточного натрия. Однако это изменение клеточного уровня часто маскируется доминирующим внеклеточным сигналом натрия, что затрудняет проведение различия между популяциями натрия с моно- и биэкспонент

Ультра-высокий контраст (УХК) MRI изображений мозгового, спинного и оптического нерва в множестве склероза с использованием непосредственно приобретенного и синтетического биполярного фильтра (BLAIR).

В этом образовательном обзоре разъясняется базовая физика, лежащая в основе использования последовательности биполярных фильтров ультравысокого контраста (UHC) (BLAIR), включая разделенное вычитаемое восстановление инверсии (dSIR). Эти последовательности могут увеличить контраст, вызванный небольшими изменениями в Т1 в коэффициенте: ten или более по сравнению с обычными IR последовательностями. В иллюстративных случаях эти последовательности использовались для лечения нескольких склерозов (MS) во время рецидива и ремиссии и сравнивались с обычным методом позиционирования (см.T2-weighted вращен

Взаимосвязь между моторными и когнитивными характеристиками и кортикальной активностью у пациентов с множественным склерозом: систематический обзор функциональных почти инфракрасных спектроскопических исследований.

Цель настоящего обзора заключалась в изучении взаимосвязи между характеристиками двигателя/когнитива и корковой активностью у лиц с множественным склерозом (МС) в условиях одноразового и двойного назначения с использованием функциональной почти инфракрасной спектроскопии (FNIRS). МЕТОДЫ: Обзор был предварительно зарегистрирован в рамках ПРОСПЕРО (CRD420261402862) и проводился в соответствии с протоколом PRISMA. На систематической основе проводились обыски в библиотеке " Пубмед " , " Кокрейн " и " Web of Science " ; 18 для решения проблем, связанных с four ключевые

[Operative neurosurgery: personal view and historical backgrounds (3). Anterior circulation--pterional approach].

Under the title of anterior circulation aneurysms and the pterional approach, followings are presented and emphasized along with mentioning their historical development in our present performance status. Pterional approach: head positioning with Mayfield-Kees fixation apparatus using one pin around the processus matoideus and the other 2 pins on the contralateral side behind the hair line the head turned 30 degrees and reclined chin-up l5-20 grade. Skin incision beginning just in front of the tragus ending up at the midline hair line in a curvilinear fashion always including the superficial te

Автоматическое отслеживание активного движения в многоразовом склерозе с использованием неконтролируемого машинного обучения

Справочная информация: Выявление активных повреждений в магнитно-резонансной съемке (MRI) имеет решающее значение для диагностики и планирования лечения множественных склерозов (MS). Активные повреждения МРТ выявляются после применения противопоказательных агентов на основе гадолиния (ГБЦА). Однако недавние исследования показали, что неоднократное применение ГБЦН приводит к накоплению ГД в тканях. Кроме того, администрация ГБКА увеличивает расходы на медицинское обслуживание. Таким образом, для повышения безопасности пациентов и снижения расходов на медицинское обслуживание важное значение име

[A challenge to primary open-angle glaucoma including normal-pressure. Clinical problems and their scientific solution].

Primary open-angle glaucoma (POAG), including normal-tension glaucoma (NTG), is reported by the Tajimi Study to afflict 3.9% of the total population, and this represents about 80% of all total glaucoma cases which, in total, afflict 5.0% of the population. We tried to analyze the clinical problems relating to POAG by looking at the pathogenesis, intraocular pressure (IOP), therapy, neuroprotection and surgery of the disease. To elucidate the pathogenesis of glaucoma progression, we measured retinal nerve fiber layer defect (RNFLD) angles', and divided the NTG cases into 2 groups, enlarged RNFL

Current approach to the diagnosis and treatment of femoral-popliteal arterial disease. A systematic review.

Peripheral arterial disease (PAD) is a common manifestation of atherosclerosis affecting 5 million adults in the United States, with an age-adjusted prevalence of 4% to 15% and increasing up to 30% with age and the presence of cardiovascular risk factors. In this article we focus on lower extremity PAD and specifically on the superficial femoral and proximal popliteal artery (SFPA), which are the most common anatomic locations of lower extremity atherosclerosis. We summarize current evidence and perform a systematic review on the diagnostic evaluation as well as the medical, endovascular and s

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… Technical improvements with digital subtraction techniques that enhance image quality as well as use of supportive modalities, such as intravascular ultrasound, angioscopy, optical coherence tomography and adjunctive hemodynamic measurements, such as pressure gradients, established catheter angiography …

Протокол для оценки региональной патологии оптического нерва грызунов с использованием продольных криосекций и поперечной электронной микроскопии.

Визуальная патология была установлена в качестве одной из главных особенностей демиелинизирующих заболеваний, таких, как множественный склероз (MS). Здесь мы представляем протокол для оценки региональной патологии оптического нерва грызунов с использованием продольных криосекций и поперечной электронной микроскопии. Мы описываем шаги для оптического диссекции нерва, постфиксации, обнаружения миелина, сканирования слайда основного белка миелина и анализа демиелинации. Затем мы подробно описываем процедуры пятна антителных флуоресцирующих веществ, анализ микроскопии для передачи электронов (ТЕА)