Результаты поиска

The vaginal microbiome composition during pregnancy in a region compromising different ethnic origins.

BACKGROUND: The vaginal microbiota plays a significant role in pregnancy outcomes and newborn health. Indeed, the composition and diversity … les were collected at each trimester: 11-13 weeks, 20-24 weeks and 28-34 weeks gestation. RESULTS: Lactobacillus species were consistently found to be dominant in the vaginal microbiota throughout all trimesters of pregnancy. Among Lactobacillus species, L. crispatus had the highest abundance in all trimesters (40.6%, 40.8% and 44.4%, respectively). L. iners w …

Evaluation of Probiotic Effects of Lactobacilli on Mutans Streptococci: An In Vitro Study.

AIM: The aim of the present study is to evaluate the probiotic effect of Lactobacillus acidophilus and Lactobacillus rhamnosus on clinical isolates of Mutans Streptococci (MS) and antibiotic susceptibility of these strains to commonly used antibiotics in dentistry. MATERIALS AND METHODS: Plaque samples from permanent first molars were collected and transferred aseptically onto Mitis-Salivarius agar and incubated at 37°C for 24 hours in the presence of 5-10% CO2. Mutans streptococci colonies were identified biochemically using Hi-Strep identification kit. The inhibitory activity of the clinica…

In vitro adhesion to enamel and in vivo colonization of tooth surfaces by Lactobacilli from a bio-yoghurt.

In the Western Scheldt Estuary near the Belgian-Dutch border, middle to late Eocene strata crop out at the current seafloor. Most vertebrae of large Eocene basilosaurid taxa from this area were previously described in several papers. They represent three morphotypes: elongated vertebrae of a large species of Pachycetus (Morphotype 1b), a not-elongated vertebra of a large 'dorudontid' basilosaurid (Morphotype 2) and 'shortened' vertebrae of a new, unnamed taxon (Morphotype 3). This article deals with a still undescribed, smaller vertebra, NMR-16642, from this site. Our first aim was to date it

Biochemical and molecular identification of lactic acid bacteria and yeasts with technological qualities from ogi (a cereal-based fermented food in Nigeria).

BACKGROUND: This study aimed to isolate, characterize, and identify lactic acid bacteria (LAB) and yeasts with technological qualities from … he API-based characterization of the nonpathogenic organisms revealed that the LAB mainly comprised Lactobacillus and Lactococcus genera, while Candida, Cryptococcus, and Trichosporon were the prominent yeast genera. However, the results for molecular identification and percentage of occurrence showed that the LAB involved in the fermentation process mainly inc …

Low-frequency magnetic fields induce oxidative and genotoxic stress and growth stimulation in lactic acid bacteria.

BACKGROUND: Low-frequency magnetic fields are increasingly encountered in environmental and industrial settings, yet their biological effects on probiotic lactic acid bacteria (LAB) remain incompletely characterized. This study evaluated the impact of direct current (DC) and alternating current (AC) magnetic fields on growth, redox homeostasis, and genomic integrity in representative LAB strains. METHODS: Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus pentosus, Lactobacillus casei, and Lacticaseibacillus paracasei were exposed to DC and AC magnetic…

Evaluation of a Plant-Based Infant Formula Containing Almonds and Buckwheat on Gut Microbiota Composition, Intestine Morphology, Metabolic and Immune Markers in a Neonatal Piglet Model.

A controlled-neonatal piglet trial was conducted to evaluate the impact of a plant-based infant formula containing buckwheat and almonds as … es was detected within each diet group in the small and large intestinal regions and fecal samples. Lactobacillus delbrueckii, Lactobacillus crispatus, and Fusobacterium sp. had higher abundance in the small intestine of plant-based formula-fed piglets compared to the dairy-based group. Bacteroides nordii, Enterococcus sp., Lactobacillus crispatus, Prevotella s …

The association between obesity and weight loss after bariatric surgery on the vaginal microbiota.

BACKGROUND: Obesity and vaginal microbiome (VMB) dysbiosis are each risk factors for adverse reproductive and oncological health outcomes in … ial component of the vaginal microbiota in obese women was characterised by a lower prevalence of a Lactobacillus-dominant VMB and higher prevalence of a high diversity (Lactobacillus spp., and Gardnerella- spp. depleted) VMB, compared with non-obese subjects (p<0.001). Obese women had higher relative abundance of Dialister species (p<0.001), Anaerococcus vagin …

Predictive functional analysis reveals inferred features unique to cervicovaginal microbiota of African women with bacterial vaginosis and high-risk human papillomavirus infection.

Mounting evidence suggests that Lactobacillus species may not necessarily be the sine qua non of healthy cervicovaginal microbiota (CVM), especially among reproductive-age African women. A majority of African women have high-diversity non-Lactobacillus-dominated CVM whose bacterial functions remain poorly characterized. Functional profiling of the CVM is vital for investigating human host-microbiota interactions in health and disease. Here, we investigated the functional potential of L. iners-dominated and high-diversity non-Lactobacillus-dominated CVM of 75 African women with and without bact…

Comparison of the bacterial species diversity of spontaneous cocoa bean fermentations carried out at selected farms in Ivory Coast and Brazil.

To compare the spontaneous cocoa bean fermentation process carried out in different cocoa-producing regions, heap and box (one Ivorian farm) … the species diversity of lactic acid bacteria (LAB) and acetic acid bacteria (AAB) was restricted. Lactobacillus fermentum and Leuconostoc pseudomesenteroides were the predominant LAB species, due to their ethanol and acid tolerance and citrate consumption. The levels of mannitol, ascribed to growth of L. fermentum, were fermentation-dependent. Also, enterobac …

Age and Breed Associated Differences in the Duodenal Microbiota of European Meat Pigeons and Shiqi Pigeons and Their Associations with Growth Traits.

The duodenum is a key site of nutrient digestion and absorption in meat pigeons, and its microbial community may be associated with host body weight and breast muscle development. In this study, the duodenal microbiota of European meat pigeons and Shiqi pigeons at 14 and 28 days of age were analyzed using 16S rRNA gene sequencing. At 14 days of age, Firmicutes abundance was significantly higher in Shiqi pigeons than in European meat pigeons, whereas Proteobacteria showed the opposite pattern. Lactobacillus constituted the most abundant microbial group in both breeds. Spearman correlation analy…

The Clinically Significant Changes in the Composition and Functional Diversity of the Vaginal Microbiome in Women with Type 2 Diabetes Mellitus.

Type 2 diabetes mellitus (T2DM) significantly influences the composition and diversity of the vaginal microbiome, with implications for mucosal immunity, infection risk, and genitourinary health. This study aimed to investigate the vaginal microbiome profiles in women with T2DM, with a focus on differences according to menopausal status and associations with Candida colonization and the use of sodium-glucose cotransporter 2 (SGLT2) inhibitors. Compared to healthy controls, women with T2DM exhibited a decreased abundance of Lactobacillus species and increased microbial diversity. Community stat…

Probiotics-Fermented Grifola frondosa Total Active Components: Better Antioxidation and Microflora Regulation for Alleviating Alcoholic Liver Damage in Mice.

Alcoholic liver damage is caused by long-term drinking, and it further develops into alcoholic liver diseases. In this study, we prepared a probiotic fermentation product of Grifola frondosa total active components (PFGF) by fermentation with Lactobacillus acidophilus, Lactobacillus rhamnosus, and Pediococcus acidilactici. After fermentation, the total sugar and protein content in the PFGF significantly decreased, while the lactic acid level and antioxidant activity of the PFGF increased. Afterward, we investigated the alleviating effect of PFGF on alcoholic liver injury in alcohol-fed mice. T…

Is the role of human female reproductive tract microbiota underestimated?

An issue that is currently undergoing extensive study is the influence of human vaginal microbiota (VMB) on the health status of women and their neonates. Healthy women are mainly colonised with lactobacilli such as Lactobacillus crispatus, Lactobacillus jensenii, and Lactobacillus iners; however, other bacteria may be elements of the VMB, particularly in women with bacterial vaginosis. The implementation of culture-independent molecular methods in VMB characterisation, especially next-generation sequencing, have provided new information regarding bacterial diversity in the vagina, revealing a…

RT-PCR-DGGE Analysis to Elucidate the Dominant Bacterial Species of Industrial Spanish-Style Green Table Olive Fermentations.

This paper describes the dominant bacterial species metabolically active through the industrial production of Spanish-style Manzanilla and Gordal olives. For this purpose, samples (brines and fruits) obtained at 0, 15, and 90 fermentation days were analyzed by a culture-independent approach to determine viable cells by reverse transcription of RNA and further PCR-DGGE analysis, detecting at least 7 different species. Vibrio vulnificus, Lactobacillus plantarum group, and Lactobacillus parafarraginis were present in samples from both cultivars; Lactobacillus sanfranciscensis and Halolactobacillu…

Evaluation of two self-care treatments for prevention of vaginal candidiasis in women with HIV.

Vaginal candidiasis (VC) is a common concern for women living with HIV infection. The authors evaluated the effectiveness of two self-care approaches to prophylaxis of VC among HIV-infected women, weekly intravaginal application of Lactobacillus acidophilus or weekly intravaginal application of clotrimazole tablets, in a randomized, double-blind, placebo-controlled trial. VC was defined as a vaginal swab positive for Candida species in the presence of signs/symptoms of vaginitis and the absence of a diagnosis of Trichomonas vaginalis or bacterial vaginosis. Thirty-four episodes of VC occurred …

Identification and characterisation of vaginal lactobacilli from South African women.

BACKGROUND: Bacterial vaginosis (BV), which is highly prevalent in the African population, is one of the most common vaginal syndromes affecting women in their reproductive age placing them at increased risk for sexually transmitted diseases including infection by human immunodeficiency virus-1. The vaginal microbiota of a healthy woman is often dominated by the species belonging to the genus Lactobacillus namely L. crispatus, L. gasseri, L. jensenii and L. iners, which have been extensively studied in European populations, albeit less so in South African women. In this study, we have therefor…

Characteristics of vaginal microbes and classification of the vaginal microbiome.

BACKGROUND: The vaginal microbiome (VMB) has been classified into several discrete community state types, some of which have been associated … iological vagitype (BVT), is proposed to classify community state types of the VMB. FINDINGS: Most Lactobacillus spp., with the exception of Lactobacillus iners , show significant correlations with host gene expression profiles and negative associations with dysbiosis-associated vaginal taxa. Many non- Lactobacillus spp. exhibit varied correlations with Lactob …

Immunochemotherapy response and its association with gut microbiota and immune profiles in advanced gastric cancer.

OBJECTIVE: To evaluate efficacy/safety/survival of PD‑1 inhibitor plus chemotherapy in advanced gastric cancer, and analyze early changes in immune cells and gut bacteria (Lactobacillus, E. coli, Bifidobacterium, Enterococcus) and their association with response. METHODS: Prospective cohort (n = 100). Responders (CR/PR/SD, n = 74) vs non‑responders (PD, n = 26) by RECIST version 1.1. Immune and bacterial levels at baseline and post‑treatment. Logistic regression and ROC. RESULTS: ORR = 36.0%, DCR = 74.0%. Median follow‑up = 8.0 mo; PFS = 11.0 mo. Responders had longer PFS (11.73 vs 2.00 mont…

Shotgun metagenomics of the vaginal microbiome in cervical shortening and preterm birth risk.

Preterm birth (PTB), a leading cause of neonatal morbidity and mortality, is frequently associated with premature cervical remodeling and va … cervical length. Species-level taxonomic profiling and functional pathway analysis revealed reduced Lactobacillus dominance, increased microbial diversity, and enrichment of non-optimal CST IV species, such as Fannyhessea vaginae, Bifidobacterium breve, and Mycobacterium canetti in the short cervix group. Functional profiling showed group differences in pathway …

Роль лактической кислотной бактерии в улучшении поведенческого дефицита, серных уровней витамина D3, B12 и снижении окислительного стресса и демиелиции в модели разложения крысы, вызванной купризоной.

Множественный склероз представляет собой хроническое, подстрекательское и демиелионирующее расстройство, затрагивающее центральную нервную систему, где, по оценкам, в мире насчитывается 2,5 миллиона человек. Появляющиеся исследования подчеркивают значительное влияние микробиота кишечника на иммунную систему, что указывает на потенциальную роль в инициировании и развитии подстрекательских заболеваний. В рамках этого исследования изучалось потенциальное терапевтическое воздействие видов лактобациля и бифидобакерия, изолированных от традиционных молочных продуктов, на демиелинизацию, вызванную ку

Совпадение в аннотации оригинала
Multiple sclerosis constitutes a chronic, inflammatory, and demyelinating disorder affecting the central nervous system, with an estimated g … progression of inflammatory diseases. This study investigated the potential therapeutic effects of Lactobacillus and Bifidobacterium species isolated from traditional dairy products on cuprizone-induced demyelination in a rat model. 48 adults male Wistar rats were randomly assigned to six groups. Demyelination was induced by daily oral administration of 0.6% ( …