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[Several yellow fever cases in an endemic area in Ivory Coast: serological and epidemiological evidence (author's transl)].

1 - More than hundred febrile haemorrhagic jaundice cases with an unexpectedly high number of death happened in the north-west of the Ivory Coast between October 1977 and December 1977. It was the end of the rainy season and in this area, yellow fever is a dreadful eventuality. 2 - Epidemiological and serological survey was immediately carried out. Presence of numerous yellow fever potential vectors was well known and has been noticed in September 1977; there were some left in December 1977. Therefore no virus was isolated either in September or further. 3 - Haemagglutination-inhibition, compl…

A yellow fever epizootic in Zika forest, Uganda, during 1972: Part 1: Virus isolation and sentinel monkeys.

The results of the yellow fever immunity survey of Central and East Africa reported by SAWYER & WHITMAN in 1936 prompted scientists to undertake well-planned epidemiological studies on yellow fever in eastern Africa. A Yellow Fever Research Institute (the present East African Virus Research Institute) was established at Entebbe in 1936 for this purpose. One of the areas where much work has been carried out is a strip of typical tropical forest, the Zika Forest, 12 kilometres from the Institute. Routine surveillance work, particularly on the biting activity of the yellow fever vector mosquitoes…

Intensive Therapeutic Plasma Exchange for Severe Yellow Fever: What Is the Evidence? Comment on Ho et al. Intensive Therapeutic Plasma Exchange-New Approach to Treat and Rescue Patients with Severe Form of Yellow Fever. Trop. Med. Infect. Dis. 2025, 10, 39.

Recent outbreaks of yellow fever in Brazil, with hundreds of cases despite available vaccination, have drawn attention to the pressing need for effective therapeutic interventions, with a special focus on the critically ill [...].

Yellow Fever in Pregnancy: A Comprehensive Review of the Clinical Implications and Vaccination in the Context of the 2024-2026 Americas Outbreak.

Yellow fever remains a major public health threat in endemic and re-emerging regions of Africa and South America, with recent outbreaks highlighting persistent gaps in prevention and surveillance. Pregnant women represent a particularly vulnerable population, yet the epidemiology, clinical impact, and preventive strategies for yellow fever in pregnancy are insufficiently characterized. Physiological and immunological changes during gestation may influence host responses to infection; however, current evidence does not demonstrate increased susceptibility to or severity of yellow fever during p…

Drought dynamics explain once in a century yellow fever virus outbreak in Brazil with implications for climate change.

While excess rainfall is associated with mosquito-borne disease because it supports mosquito breeding, drought may also counterintuitively increase disease transmission by altering mosquito and host behavior. This phenomenon is important to understand because climate change is projected to increase both extreme rainfall and drought. In this study, we investigated the extent to which seasonally-driven mosquito and primate behavior drove the first yellow fever virus (YFV) epidemic in an urban area in Brazil in nearly a century, coinciding with an equally rare drought, and to assess the role of i…

NS5 Region Cloning Protocol, for the Construction of an Accurate Standard Curve in the Detection and Diagnosis of the Yellow Fever Virus.

The yellow fever virus (YF), belonging to the Flaviviridae family, has an RNA genome of 10,862 nucleotides and continues to cause significant epidemics in Africa and South America despite an effective vaccine. Timely laboratory identification is essential for emergency immunization campaigns, but delays often lead to unconfirmed outbreaks. The presence of other diseases with similar symptoms complicates differential diagnosis, particularly in endemic regions, and the recent resurgence of YF activity has renewed interest in this arboviral disease. Genomic DNA (gDNA) and plasmids with cloned tar…

Community perceptions of yellow fever and its treatment practices in regions with reported outbreaks in Uganda: A qualitative study.

BACKGROUND: Yellow fever (YF), a mosquito-borne viral hemorrhagic fever, is endemic to Uganda and has caused numerous outbreaks in recent years. This study explored local perceptions of YF outbreaks among vulnerable groups in Uganda to inform future public health campaigns. METHODOLOGY: A qualitative study examined community perceptions of YF and its treatment practices. Data were collected in six districts where YF outbreaks were reported in 2010 and 2016. A total of 76 individuals participated, comprising 43 semi-structured interviews, 10 expert interviews, and 4 focus group discussions, in…

The development and validation of a microneutralization assay for the detection and quantification of anti-yellow fever virus antibodies in human serum.

UNLABELLED: The plaque reduction neutralization test (PRNT) has been used widely for the detection and quantitation of yellow fever (YF) virus-neutralizing antibodies in human serum; however, it is labor-intensive and challenging to adapt to high-throughput clinical testing needed for vaccine licensure. Here, we describe the development and validation of a new Vero cell-based YF microneutralization (MN) assay, with immunostaining readout, for the detection and quantification of YF virus-neutralizing antibodies in human serum. Comparison of neutralizing antibody titers measured with the YF MN a…

Трансгенная линия для характеристики выражения GABA-рецептор для изучения нейронной основы olfaction в комаре желтой лихорадки.

Комари Aedes aegypti — важный переносчик болезней, в том числе денге, зика, чикунгунья и желтая лихорадка. Olfaction — это важнейшая форма для комаров, позволяющая им найти хозятники, источники нектара и места для овипозиционирования. GABA является основным нейротрансмиттером в обонятельной обработке мозга насекомых, включая первичный центр обоняния, антенну. Предыдущая работа с Ae. aegypti позволяет предположить, что к обработке запахов может быть применена ингибиция антенн. Однако мало что известно о выражении рецептора GABA в мозгу комара или о том, как они могут быть вовлечены в притяжение

Совпадение в аннотации оригинала
The mosquito Aedes aegypti is an important vector of diseases including dengue, Zika, chikungunya, and yellow fever. Olfaction is a critical modality for mosquitoes enabling them to locate hosts, sources of nectar, and sites for oviposition. GABA is an essential neurotransmitter in olfactory processing in the insect brain, including the primary olfactory cen…

Risk of Arboviral Transmission and Insecticide Resistance Status of Aedes Mosquitoes during a Yellow Fever Outbreak in Ghana.

BACKGROUND: In late 2021, Ghana was hit by a Yellow Fever outbreak that started in two (2) districts in the Savannah region and spread to several other Districts in (3) regions (Oti, Bono and Upper West).Yellow fever is endemic in Ghana. However, there is currently no structured vector control programme for the yellow vector, Aedes mosquitoes in Ghana. Knowledge of Aedes bionomics and insecticide susceptibility status is important to control the vectors. This study therefore sought todetermine Aedes vector bionomics and their insecticide resistance status during a yellow fever outbreak. METHO…

Risk of arboviral transmission and insecticide resistance status of Aedes mosquitoes during a yellow fever outbreak in Ghana.

BACKGROUND: In late 2021, Ghana was hit by a Yellow Fever outbreak that started in two districts in the Savannah region and spread to several other Districts in three regions. Yellow fever is endemic in Ghana. However, there is currently no structured vector control programme for Aedes the arboviral vector in Ghana. Knowledge of Aedes bionomics and insecticide susceptibility status is important to control the vectors. This study therefore sought to determine Aedes vector bionomics and their insecticide resistance status during a yellow fever outbreak. METHODS: The study was performed in two y…

Evaluation of safety and immuno-efficacy of a next generation live-attenuated yellow fever vaccine in cynomolgus macaques.

The increased demand for yellow fever (YF) vaccines over the last decade, along with insufficient availability of specific pathogen-free embryonated eggs required for timely vaccine production, has led to global YF vaccine shortages. A new live-attenuated YF vaccine candidate (generically referred to as vYF) cloned from a YF-VAX® vaccine (YF-17D vaccine) substrain adapted for growth in Vero cells cultured in serum-free media is currently in development. Here, we assessed the safety and immunogenicity of vYF, and its protective activity upon virulent challenge with wild-type yellow fever virus …

Standardization, validation, and comparative evaluation of a faster and high-performance test for quantification of yellow fever neutralizing antibodies.

Although it is considered the reference for quantification of neutralizing antibodies, classical method of the plaque reduction neutralization test (PRNT) is labor intensive, requires specific equipment and inputs, besides a long time for its finalization, even in the micro-PRNT version (in 96-well plates). It has a higher sample throughput, however the smaller wells make the reading of plaques more difficult. With an immunoenzymatic revelation step and a semi-automated reading, the μFRN-HRP (micro Focus Reduction Neutralization - Horseradish Peroxidase) is a faster and more efficient test for

Evidence for serial founder events during the colonization of North America by the yellow fever mosquito, Aedes aegypti.

The Aedes aegypti mosquito first invaded the Americas about 500 years ago and today is a widely distributed invasive species and the primary vector for viruses causing dengue, chikungunya, Zika, and yellow fever. Here, we test the hypothesis that the North American colonization by Ae. aegypti occurred via a series of founder events. We present findings on genetic diversity, structure, and demographic history using data from 70 Ae. aegypti populations in North America that were genotyped at 12 microsatellite loci and/or ~20,000 single nucleotide polymorphisms, the largest genetic study of the r…