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Mitochondria-targeted photodynamic nanoparticles boost antitumor immunity by suppressing mitophagy in osteosarcoma.

Osteosarcoma (OS) responds poorly to immunotherapy owing to its highly immunosuppressive phenotype. Photodynamic therapy (PDT) can induce mitochondrial damage by generating reactive oxygen species (ROS), thereby triggering immunogenic cell death (ICD) and activating antitumor immunity. However, mitochondrial damage readily activates mitophagy, which attenuates oxidative stress and compromises therapeutic efficacy. In this study, we construct a multifunctional nanoparticle (TPSM@IT-4Cl), which co-loads the photosensitizer IT-4Cl and the mitochondrial fission inhibitor Mdivi-1 and can target mit

Bimetallic nanoparticles mediated plasmonic enhanced cardiac electrophysiology by cell based excitation biosensor.

Bradyarrhythmia is a life-threatening cardiac disease due to its intricate pathophysiology and limited therapeutic options. Conventional clinical approaches, including pharmacotherapy and pacemaker implantation, are limited by drug resistance, surgical complications, and the absence of real-time treatment evaluation capabilities. To overcome these challenges, we develop an integrated biosensing-regulating platform capable of simultaneous in situ NIR-II photothermal treatment and electrophysiological signal recording. The platform employs branched Au/Ag bimetallic nanoparticles (NPs) as phototh…

Denosumab-functionalized nanoparticles target macrophages to mitigate age-related osteoarthritis and osteoporosis.

Osteoarthritis (OA) and osteoporosis (OP) are prevalent degenerative diseases in the elderly population, often coexisting and sharing a comm … s, we have prepared folate-modified liposomes (Lip-FA) loaded with DS (DS@Lip-FA) target macrophage nanoparticles. Using humanized mouse models of aging, we observed DS@Lip-FA effects on macrophage polarization and the subsequent impact on cartilage and bone tissues. Our results reveal that aging led to an increase in the pro-inflammatory M1 macrophages and a d …

Intranasal CRISPR lipid nanoparticles targeting MAPK9 attenuate neuroinflammation after traumatic brain injury.

Traumatic brain injury (TBI) induces a sustained neuroinflammatory response involving activated microglia and infiltrating myeloid cells, contributing to secondary brain damage and long-term neurological dysfunction. Modulating these inflammatory responses toward a more reparative phenotype represents a promising therapeutic strategy, but achieving targeted delivery within the injured brain remains a major challenge. Here, we developed a targeted, non-viral gene-editing platform using lipid nanoparticles (LNPs) encapsulating CRISPR-Cas12a components directed against MAPK9, a key mediator of in…

Intravenously Delivered Lipid Nanoparticles Access Acute Spinal Cord Injury via Disrupted Vasculature.

Trauma to the spinal cord disrupts the blood-spinal cord barrier and triggers a secondary injury cascade characterized by inflammation and p … s transient protein production. Here, we investigated whether intravenously administered mRNA-lipid nanoparticles could leverage the injury-induced disruption of the blood-spinal cord barrier to access the injured spinal cord for local transgene expression. After spinal cord injury in a rat, lipid nanoparticles loaded with reporter mRNA were administered intrav …

Magnetically frustrated calcium-substituted SrMn2Fe4O11 R-type hexaferrite nanoparticles for biomedical applications.

This research project was based on the idea of inducement of more magnetic frustration in already frustrated R-type hexagonal ferrite by the … magnetic fields, they become better candidates to be used for biomedical applications. Also, these nanoparticles are characterized to obtain their cytotoxicity, antibacterial activities, and biodistribution properties. The antibacterial analyses were performed on two bacteria Escherichia coli (E. coli), and Staphylococcus aureus (S. aureus) by agar well diffus …

Green synthesis of spheroid silver nanoparticles using cyanobacteria with multifunctional biological activities.

Cyanobacteria-mediated synthesis of silver nanoparticles (AgNPs) offers a sustainable approach for producing functional AgNPs for biomedical applications. However, controlling their physicochemical properties while maintaining robust biological activities remains a critical challenge. Here, we developed a green, one-pot synthesis method to produce uniform, small AgNPs using the novel Cyanobium gracile PCC 6307 and evaluated their biological activities. PCC 6307 was isolated and identified using microscopic examination and sequencing analysis. The aqueous extract of algal biomass (CB) and cell-…

Inhalation of cadmium oxide nanoparticles alters intestinal and pulmonary microbiomes in mice.

Exposure to cadmium (Cd), a toxic heavy metal, is a severe threat to organismal health, causing a wide range of pathological alterations in … mize the toxicity of heavy metals in future therapeutic approaches. KEY POINTS: • Inhalation of CdO nanoparticles significantly alters both gut and pulmonary microbiome composition and diversity in mice. • Microbiome changes are more pronounced in the gut than in the lungs following inhalation exposure. • Partial recovery of microbiome composition occurs after …

Ionizable Cholesterol-Integrated Lipid Nanoparticles for Efficient siRNA Delivery to Solid Tumors.

Lipid nanoparticles (LNPs) are clinically validated platform for the delivery of small interfering RNA (siRNA) therapeutics. However, conventional LNPs typically consist of multiple lipid components, which complicates formulation optimization and limits compositional flexibility. Furthermore, efficient delivery of siRNA to extrahepatic tissues, including solid tumors, remains a major challenge. To address these limitations, we developed a class of ionizable cholesterol derivatives by conjugating biocompatible dimethylated amino acids to cholesterol through a cleavable linker, thereby integrati…

Research Progress of Ferrosoferric Oxide Nanoparticles in Bone Regeneration and Disease Treatment.

Ferrosoferric oxide nanoparticles (Fe3O4NPs), with unique magnetic properties and biocompatibility, have shown great promise for application in the treatment of bone regeneration and diseases in recent years. Bone-related diseases, such as osteoporosis, bone defects and bone tumors, seriously affect the health and quality of life of millions of people around the world, and existing treatments have many limitations, such as low bioavailability, significant side effects, and lack of precision in drug delivery. Fe3O4NPs could realize precise magnetic targeting therapy through an external magnetic…

Selenium nanoparticles modulate gut-brain axis via NRF2 to attenuate Parkinsonian neurotoxicity.

Parkinson's disease (PD) is characterized by progressive dopaminergic neurodegeneration driven by mitochondrial dysfunction, oxidative stress, neuroinflammation, and impaired gut-brain communication. Here, we report a biogenic selenium nanoparticle (Se-NP) platform derived from mussel tissue and demonstrate its nano-enabled neuroprotective efficacy in a rotenone-induced zebrafish model of Parkinsonian neurotoxicity. Selenium was extracted from the tissue of Perna viridis (mussel) and used for the biogenic synthesis of Se-NPs through a green reduction approach under controlled conditions. The m

Biomimetic astragaloside-IV-loaded mesoporous silica nanoparticles for treatment of dilated cardiomyopathy.

Dilated cardiomyopathy (DCM) is currently the most prevalent form of cardiomyopathy; it poses a severe threat to human health and is characterized by poor prognosis and high mortality. In this study, we prepared macrophage-membrane-coated and astragaloside-IV-loaded mesoporous silica nanoparticles (AS@MSN/TA/MM) and assessed their therapeutic effects in vitro and in vivo. The designed AS@MSN/TA/MM were spherical and had an average particle size of approximately 234 nm, a zeta potential of -24.5 mV, a drug loading capacity (LC) of 9.5% ± 0.04%, and an encapsulation efficiency (EE) of 98.9% ± 0.…

Development of hematopoietic stem cell-targeted lipid nanoparticles through lipid composition optimization.

Hematopoietic stem cell (HSC)-targeted gene editing holds significant potential for treating hereditary hematopoietic disorders, yet the efficient and safe delivery of gene-editing tools into HSCs remains a critical challenge. Lipid nanoparticles (LNPs) have emerged as a promising platform for nucleic acid delivery; however, achieving high transfection efficiency in HSCs remains challenging. In this study, we developed HSC-targeted LNPs by integrating Bayesian optimization with our functional amino lipids. The optimized LNPs exhibited markedly improved transfection efficiency while preserving …

Silica-installed redox nanoparticles enable effective oral silymarin therapy for liver fibrosis.

Liver fibrosis is a chronic pathological condition caused by repeated or sustained liver injury, for which effective pathological treatments remain limited. Silymarin, a hydrophobic compound with anti-inflammatory, antioxidant, and antifibrotic properties, has shown therapeutic potential. However, its clinical application is restricted by poor aqueous solubility and low gastrointestinal (GI) bioavailability. In this study, we developed silica-containing redox nanoparticles (siRNP) as an oral nanocarrier system to improve the delivery of silymarin to the liver. Silymarin-loaded siRNP (Sil@siRNP…

Metal oxide nanoparticles for acne vulgaris: mechanisms, synthesis, formulation, and translational prospects.

Acne vulgaris is a chronic inflammatory condition of the pilosebaceous unit resulting from follicular hyperkeratinization, increased sebum p … earch for non-antibiotic strategies that preserve the microbiome. This review evaluates metal oxide nanoparticles as multifunctional agents with acne-targeted, anti-inflammatory, antioxidant, and photocatalytic activities suitable for the follicular microenvironment. The emphasis is placed on zinc oxide, titanium dioxide, copper oxide, iron oxide, magnesium oxi …

Ionic organic molecular cage-supported Pd nanoparticles for aqueous Suzuki-Miyaura coupling.

Spiroborate-linked organic molecular cages bearing bipyridine and phenanthroline ligands stabilize palladium nanoparticles whose dimensions are consistent with exocyclic growth. The cage-supported Pd nanoparticles act as efficient, recyclable catalysts for aqueous Suzuki-Miyaura coupling, affording high conversions under mild conditions while maintaining cage integrity, nanoparticle morphology, and activity over multiple cycles.

Polyphenol-functionalized silver nanoparticles promote differential remineralization and reinforcement of demineralized dentin.

OBJECTIVES: This study aimed to synthesize and characterize AgNPs functionalized with crosslinking polyphenols extracted from grape seed extract (GSE) and green tea leaves (GT), and to evaluate their biocompatibility and their effects on the mechanical and physicochemical properties of demineralized dentin. METHODOLOGY: AgNPs were characterized by TEM, XRD, TGA, and zeta potential analysis to determine particle morphology, crystalline structure, chemical composition, and surface charge. Biocompatibility was assessed using fibroblast cytotoxicity assays. In total, 40 human mid-coronal dentin s

Sodium hyaluronate/lysine nanoparticles loaded with si-VEGF-A inhibits corneal neovascularization.

OBJECTIVE: This study designed sodium hyaluronate/lysine (SH/Lys)-based nanoparticles loaded with vascular endothelial growth factor A (VEGF-A) siRNA (V-SH/Lys), aiming to effectively treat corneal neovascularization (CNV) by specifically inhibiting the expression of VEGF-A. METHODS: The particle size, zeta potential and stability of the V-SH/Lys nanoparticles loaded with si-VEGF-A were tested. The effects of V-SH/Lys nanoparticles on the tube formation and migration of vascular endothelial cells were evaluated through Transwell, cell scratch assay and cell tube formation assay. A rat model o…

Преобразовательный потенциал липидных наночастиц, приспособленных для острой миэлоидной лейкемии.

Технологи, которые занимаются клетками, и инженерная клеточная терапия до сих пор не принесли таких же впечатляющих результатов в острой миэлоидной лейкемии (АМЛ), что и в других гематологическая малигнации. В ходе этого обзора мы анализируем проблемы, связанные с разработкой иммунотерапии для борьбы с отмыванием денег, включая целевую, неопухоль, синдром выброса цитокина (КРС) и «миелиоидный поглотитель». Затем мы обсуждаем появление двух технологий, которые могли бы решить основные проблемы, связанные с проведением расследований по борьбе с отмыванием денег: целенаправленные липидные наночас

Совпадение в аннотации оригинала
T-cell engagers and engineered cell therapies have yet to deliver the same spectacular results in acute myeloid leukemia (AML) that they hav … gies that could address the major challenges with investigational AML therapeutics: targeted, lipid nanoparticles (LNPs) loaded with specific mRNAs and a type of synthetic logic gate (a NOT gate). This approach builds on recent advancements of mRNA-loaded LNPs that developed with the COVID19 vaccines and evolved into vehicles for delivery to T cells. The LNP mo …

Antiparasitic effect of pomegranate peel extract-loaded chitosan nanoparticles on Blastocystis spp.

Blastocystis species (spp.), cosmopolitan anaerobic protists, are recently associated with inflammatory bowel diseases, and colorectal cancer. Serious side effects, variable efficacy in different subtypes, and rising resistance against the sole drug of choice, metronidazole (MTZ), necessitate a search for safer, effective alternatives. Thus, the present work aims to determine the total phenolic content and the reducing power of pomegranate peel extract (PPE) and testing its efficacy either alone or loaded with chitosan nanoparticles (PPE CSNPs) against Blastocystis spp. in vitro and in vivo co…