Результаты поиска

Scorpine, an anti-malaria and anti-bacterial agent purified from scorpion venom.

A novel peptide, scorpine, was isolated from the venom of the scorpion Pandinus imperator, with anti-bacterial activity and a potent inhibitory effect on the ookinete (ED(50) 0.7 microM) and gamete (ED(50) 10 microM) stages of Plasmodium berghei development. It has 75 amino acids, three disulfide bridges with a molecular mass of 8350 Da. Scorpine has a unique amino acid sequence, similar only to some cecropins in its N-terminal segment and to some defensins in its C-terminal region. Its gene was cloned from a cDNA library.

Biosynthesis and Export of Bacterial Glycolipids.

Complex carbohydrates are essential for many biological processes, from protein quality control to cell recognition, energy storage, and cell wall formation. Many of these processes are performed in topologically extracellular compartments or on the cell surface; hence, diverse secretion systems evolved to transport the hydrophilic molecules to their sites of action. Polyprenyl lipids serve as ubiquitous anchors and facilitators of these transport processes. Here, we summarize and compare bacterial biosynthesis pathways relying on the recognition and transport of lipid-linked complex carbohydr

Evaluation of an automated blood culture detection system for sterility testing of cellular and gene therapy products.

UNLABELLED: Sterility testing of cellular and gene therapy products is required to ensure safe infusion for the recipient. Sterility testing … ted using either formulation or a saline-only growth control inoculated with Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Clostridium sporogenes, Candida albicans, or Aspergillus brasiliensis. Contrived specimens were prepared in blood culture sets consisting of an aerobic bottle and an anaerobic bottle and incubated for a maximum of 14 days. B …

Fermented purple onion (Allium cepa L.) and chive (Allium schoenoprasum) bulb extracts as antibiotic alternatives against toxin-carrying bacteria: In vitro and pathogenicity assessment in chickens.

BACKGROUND: Antibiotic resistance in Escherichia coli and Salmonella spp. poses a significant threat to poultry health and productivity. Ove … ing. METHODS: We fermented fresh purple onion and chive extracts using Lactobacillus plantarum and Bacillus subtilis for 72 hours under anaerobic conditions. Pathogenic E. coli (22) and Salmonella spp. (9) were isolated from 30 diarrheal chicken fecal samples and identified through biochemical tests, in vivo virulence testing in chicks, polymerase chain reaction d …

Blanching Pre-Treatment Promotes High Yields, Bioactive Compounds, Antioxidants, Enzyme Inactivation and Antibacterial Activity of 'Wonderful' Pomegranate Peel Extracts at Three Different Harvest Maturities.

'Wonderful' pomegranate (Punica granatum L.) peel contains a wide range of phytochemicals including vitamins, dietary fibre, phenolic compou … µg/mL against all four bacteria strains tested, which included two Gram-positive bacterial strains (Bacillus subtilis ATCC 6051 and Staphylococcus aureus ATCC 12600) and two Gram-negative bacteria (Escherichia coli 11775 and Klebsiella pneumonia ATCC 13883). A total of 25 metabolites including phenolic acids (4), organic acids (1), flavonoids (4), ellagitannins (13 …

Some novel rare earth metal ions complexes: Synthesis, characterization, luminescence and biocidal efficiency.

New lanthanide complexes (1-3) of the general formulae [Ln(L)(NO3)(H2O)] have been synthesized by reaction of Ln(NO3)3 {Ln = La (1), Sm (2) and Yb (3)} with 2,2'-(((1E,1'E)-thiophene-2,5-diylbis(methaneylylidene))bis(azaneylylidene))diphenol (H2L). Based on elemental analysis, spectroscopic studies (UV-Vis., FT-IR, ESI-MS, 1H/13C NMR), molar conductance and thermogravimetric analysis, the Schiff base ligand was suggested to coordinate Ln(III) ions through the azomethine nitrogens, deprotonated hydroxyl groups, and thiophene sulphur atom. The interaction of the synthetic compounds with CT-DNA h

Wound dressing of chitosan-based-crosslinked gelatin/ polyvinyl pyrrolidone embedded silver nanoparticles, for targeting multidrug resistance microbes.

Wound dressing composed of chitosan, based crosslinked gelatin/ polyvinyl pyrrolidone, embedded silver nanoparticles were fabricated using solution casting method. The membrane was characterized by FTIR, SEM and TGA. Glutaraldehyde (0.5 %) was used for the crosslinking of membrane components and associated with 7-folds boosted mechanical performance, 28 % more hydrolytic stability, 3-folds thickness reduction and morphological roughness. Silver nanoparticles were characterized by UV-vis, XRD and TEM for an average size of 9.9 nm. The membrane with higher concentration of silver nanoparticles s

Novel nanocomposite derived from ZnO/CdS QDs embedded crosslinked chitosan: An efficient photocatalyst and effective antibacterial agent.

A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker]. The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD)

Antibacterial and antiviral naphthazarins from Maharanga bicolor.

Maharanga bicolor, Boraginaceae, is used in the Nepalese ethnomedicine for the treatment of several diseases. In the course of screening investigations the dichloromethane extract of the roots of Maharanga bicolor was found to inhibit the growth of gram positive bacteria. Bio-assay directed fractionation led to the isolation of five active naphthazarins, deoxyalkannin (1), alkannin (2), acetylalkannin (3), alkannin beta-hydroxyisovalerate (4) and alkannin beta-acetoxyisovalerate (5). Compounds 2-5 showed antibacterial activity against multi resistant human pathogenic Staphylococcus and Enteroc

Адаптация эукариот-подобных PRS к прокариот-подобным tRNAPro.

Синтеты Проли-ТРНА (ПРОРС) уникальны среди аминоачил-тРНК (ААРС) two различные структурные архитектуры различных организмов: прокариота-подобные (П-тип) и эукариоте/археон-подобные (Е-тип). Интересно, что Бакиллус Тюрингьенси гавань обоих типов, сосуществует P-тип (BtProRS1) и E-тип PRS (BtProRS2). Несмотря на свои различия, оба фермента формально выражаются и функционируют в vivo. По аналогии с BtProRS1, BtPRS2 выборочно заряжает TRNAPro типа P и демонстрирует более высокий допуск к галоугинону, чем каноническая PRS. Однако эти two Изозимы признают основные идентификационные элементы типа P t

Совпадение в полном тексте источника
… Another scenario was found in Bacillus subtilis , where a second, specialized tyrosyl-tRNA synthetase (TyrZ) is induced to protect cells against high concentrations of D-Tyr. Unlike the housekeeping enzyme TyrS, TyrZ exhibits significantly higher selectivity for L-Tyr over D-Tyr. … The Bacillus subtilis tyrZ gene encodes a highly selective tyrosyl-tRNA synthetase and is regulated by a MarR regulator and T box riboswitch. J. Bacteriol. 2015; 197:1624–1631. 47. Nakamura Y., Ito K.. Control and function of lysyl-tRNA synthetases: diversity and co-ordination. Mol. …

Phytochemical Composition and Bioactivity of Ultrasound-Assisted Ethanolic Extract of Ceratopteris Thalictroides (L.) Brongn: Integrated In Vitro and In Silico Evaluation of Antibacterial, Anticancer, and Anti-Inflammatory Studies.

Ferns and allies are underexplored sources of bioactives. This study evaluates the phytochemical composition and bioactivities of the ultrasound-assisted ethanolic (CT-UAE) extract of the dietary fern Ceratopteris thalictroides (L.) Brongn (C. thalictroides). The extract contained notable levels of total phenolics (4.34 ± 0.16 mg GAE/g), flavonoids (3.74 ± 0.08 mg QE/g), and alkaloids (1.27 ± 0.15 mg AE/g). Volatile and semi-volatile compounds were identified using GC-MS/MS, while LC-MS/MS profiling revealed non-volatile and polar metabolites. The extract exhibited strong antibacterial activit

Microorganisms Associated with the Marine Sponge Scopalina hapalia: A Reservoir of Bioactive Molecules to Slow Down the Aging Process.

Aging research aims at developing therapies that delay normal aging processes and some related pathologies. Recently, many compounds and extracts from natural products have been shown to slow aging and/or extend lifespan. Marine sponges and their associated microorganisms have been found to produce a wide variety of bioactive secondary metabolites; however, those from the Southwest of the Indian Ocean are much less studied, especially regarding anti-aging activities. In this study, the microbial diversity of the marine sponge Scopalina hapalia was investigated by metagenomic analysis. Twenty-s

Совпадение в полном тексте источника
… The iturins are mainly produced by members of the Bacillus subtilis group; however, the production of iturins is strongly associated with B. amyloliquefaciens [ 57 ]. … Secondary metabolite production and the safety of industrially important members of the Bacillus subtilis group. FEMS Microbiol. Rev. 2018;42:721–738. doi: 10.1093/femsre/fuy028. 58. Mondol M.A.M., Shin H.J., Islam M.T. …

Oliveria decumbens Vent. (Apiaceae): Biological screening and chemical compositions.

ETHNOPHARMACOLOGICAL RELEVANCE: Oliveria decumbens Vent. (Apiaceae), a single aromatic species in Iran, is traditionally used for healing in … ure compounds. The bioassays were as follows: antibacterial activity against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhi (using microplate alamar blue assay; MABA), antifungal activity against Aspergillus niger, A. fumigatus, Candida albicans, C. glabarate, Fusarium lini, Microspurum canis, and Trichophyt …

Антимикробный спектр деятельности и механизм действий линейных альфа-гелипических Пептидов, вдохновляемых креветочными антилиполисахаридами.

Креветочные антилипополизакхаридные факторы (АФО) образуют многофункциональное и разнообразное семейство противомикробных защитных пептидов (АМП), состоящих из seven Члены (группы А-G), которые различаются по своей первичной структуре и биохимическим свойствам. Это амфипатические пептиды с two Сохраненные остатки цистейна стабилизируют центральный β-волокн, который, как считается, является основным районом их биологической деятельности. В этом исследовании мы синтезировали three Линейные (без цистейна) пептиды, основанные на аминокислотной последовательности центральных β-волокн недавно иденти

Совпадение в полном тексте источника
… Aspergillus brasiliensis ATCC 16404, Bacillus subtilis ATCC 6633, Candida krusei ATCC 6258, Candida parapsilosis ATCC 22019, Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus ATCC 25932 and ATCC 29737, Trichoderma virens ATCC 9645, Vibrio alginolyticus ATCC 17749 … Isolation and Characterization of Antimicrobial Peptides Derived from Bacillus Subtilis E20-Fermented Soybean Meal and Its Use for Preventing Vibrio Infection in Shrimp Aquaculture. Fish Shellfish. Immunol. 2017;67:270–279. doi: 10.1016/j.fsi.2017.06.006. 39. Strøm M.B., Rekdal O., Svendsen J.S. …

Biopesticides from plants: Calceolaria integrifolia s.l.

The effects of persistent organic pollutants (POPs) on humans and biodiversity are multiple and varied. Nowadays environmentally-friendly pe … richia coli, Enterobacter agglomerans, and Salmonella typhi, and against the Gram-positive bacteria Bacillus subtilis, Sarcinia lutea, and Staphylococcus aureus and their effects compared with those that of kanamycin. Mixtures of isorhamnetin/dunnione/kaempferol/ferulic/gallic acid in various combinations were found to have the most potent bactericidal and fungicid …

Известная эволюция полимер-связывающего пептида LCI для улучшения связывания полипропилена.

Функционализация поверхностей полимеров с помощью полимерных связывающих пептидов предоставляет значительные возможности для направленногоImmobilization ферментов, биоактивных пептидов и антигенов. Применение полимерных связывающих пептидов в качестве адгезивов требует надежного и стабильного связывания при условиях процесса. Молекулярные режимы взаимодействия между материалами поверхностей, пептидами и растворителем часто не понимаются на уровне, который позволяет (semi-) рационально проектировать полимерные связывающие пептиды, что мешает полному использованию их потенциала. Концепция направ

Совпадение в полном тексте источника
… The PP-binding peptide LCI (originating from Bacillus subtilis [ 23 ]) was improved in PP-binding strength through one round of directed evolution to validate the PePevo [ 17 ]. … Solution Structure of LCI, a Novel Antimicrobial Peptide from Bacillus subtilis. Biochemistry. 2011;50:3621–3627. doi: 10.1021/bi200123w. 24. Singh S.K., Kishore N. Thermodynamic insights into the binding of triton X-100 to globular proteins: A calorimetric and spectroscopic investigation. J. …

Biological Activity, Apoptotic Induction and Cell Cycle Arrest of New Hydrazonoyl Halides Derivatives.

BACKGROUND: The hydrazonoyl halides are presently an important target in the field of medicinal chemistry. The interest in the chemistry of … 2e and 2f exhibited high activity against two tested gram positive bacteria Staphylococcus aureus, Bacillus subtilis, and gram negative ones Escherichia coli, and Pseudomonas aeruginosa, the rest of the compounds were found to be moderately active against the tested microorganisms. Regarding their antifungal effect, compound 2c exhibited potent and promising effec …

Deciphering the Potentials of Cardamom in Cancer Prevention and Therapy: From Kitchen to Clinic.

Cardamom (cardamum) is a spice produced from the seeds of several Elettaria and Amomum plants of the Zingiberaceae family. Cardamom has been demonstrated to offer numerous benefits, including its antioxidant, antimicrobial, anti-inflammatory, and other metabolic (anti-diabetic) properties, and its potential to reduce cancer risk. Recently, researchers have extracted and tested multiple phytochemicals from cardamom to assess their potential effectiveness against various types of human malignancy. These studies have indicated that cardamom can help overcome drug resistance to standard chemothera

Совпадение в полном тексте источника
… Name of Bacteria | Reference Helicobacter pylori | [ 133 ] Escherichia coli , Salmonella typhi , Bacillus cereus , Bacillus subtilis , Streptococcus pyogenes , and Staphylococcus aureus | [ 134 ] Staphylococcus aureus and Proteus Mirabilis | [ 135 ] Klebsiella pneumoniae , Pseudomonas aeruginosa , Salmonella … typhi , Shigella dysenteriae , Shigella sonnei , Staphylococcus aureus , Streptococcus-β-haemolytica , Bacillus subtilis , B. megaterium , and Sarcina lutea | [ 136 ] Staphylococcus aureus , Streptococcus pneumonia , S.epidermidis , P. aeroginosa , K. pneumonia , Proteus mirabilis , Enterobacter spp …

[Antibacterial activity of the antifungal peptide Mt6 - 21DLeu derived from Musca domestica against Acinetobacter baumannii and the underlying mechanisms].

OBJECTIVE: To investigate the antibacterial activity of the antifungal peptide Mt6-21DLeu derived from Musca domestica against Acinetobacter baumannii (AB) and unravel its underlying mechanisms, so as to provide insights into development of novel agents against AB. METHODS: The minimum inhibitory concentrations (MICs) of Mt6-21DLeu, M. domestica-derived antifungal peptide-1 (MAF-1A), and polymyxin B were determined against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and AB using the broth microdilution assay, and the antibacterial …

Sophorolipids as anticancer agents: progress and challenges.

Sophorolipids (SLs) are considered effective biosurfactant for cancer treatment, which can efficiently inhibit the viability of various cancer types including breast, lung, liver, cervical and colon cancers. Their mechanism of action targets apoptosis and operates at the level of caspase enzymes, upregulation and downregulation of the B-cell lymphoma (Bcl)-family proteins, and changes in mitochondrial membrane permeability. The binding of SLs to the cancer cell receptors modulates the expression of Bax, APAF1, Bcl-2 and Bcl-x, and triggers the release of cytochrome c into the cytosol which fur

Совпадение в полном тексте источника
… SLs have been reported to exhibit antibacterial activity against both Gram positive and Gram negative bacteria Bacillus subtilis and Escherichia coli , generating ROS [ 27 ]. … Disruption of biofilms of several bacteria including Bacillus subtilis and S. aureus by SLs through elimination of cellular element and the release of intracellular contents have been reported [ 43 ]. …