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Exploring Relationships Within the Microbiome of Root Canal Infections and the Influence of Associated Clinical Parameters.

AIM: To identify relationships among bacterial species in endodontic infections, to determine their core microbiome, and associated clinical … dodontic core microbiome included Parvimonas micra, Streptococcus sanguinis, Enterococcus faecalis, Porphyromonas endodontalis, Prevotella nigrescens and Fusobacterium nucleatum. Symptomatic cases had a core microbiome of Bacteroidaceae.G.1. sp oral taxon 272 and Haemophilus parainfluenzae. Sinus cases had a core microbiome of Bacteroidaceae.G.1. sp oral taxon …

A Novel Micro/Nano-Roughened Self-Glazed Zirconia Implant With Enhanced Osseointegration and Satisfactory Soft Tissue Sealing: An In Vitro and In Vivo Study.

OBJECTIVES: This study aimed to develop a novel self-glazed zirconia (SZr) dental implant featuring micro/nano-roughened thread and polished neck, and to examine its properties both in vitro and in vivo. MATERIAL AND METHODS: The micro/nano-roughened SZr surfaces (SZr-MN) were manufactured using sandblasting and chemical vapor deposition. Surface topography, elemental composition, roughness, and contact angle were systematically characterized. Streptococcus mutans and Porphyromonas gingivalis were used to evaluate bacterial adhesion. Bone marrow mesenchymal stem cells (BMSCs) and human immort…

Ferritin promotes monocyte osteoclastogenic differentiation to aggravate alveolar bone resorption in periodontitis.

BACKGROUND/PURPOSE: Alveolar bone resorption in periodontitis critically impairs masticatory function and tooth stability, yet the mechanist … ro mechanistic studies where THP-1 monocytes (human acute monocytic leukemia cell line) primed with Porphyromonas gingivalis lipopolysaccharide (P. gingivalis-LPS) were analyzed for ferritin/receptor activator of nuclear factor-κB ligand (RANKL) secretion, and RAW264.7 pre-osteoclasts (murine macrophage-like cell line) were treated with apoferritin ± RANKL to a …

Diagnostic significance of gut Microbiome dysbiosis and biomarker expression in Egyptians with hepatocellular carcinoma.

Egypt has the greatest incidence of hepatitis C virus (HCV) infection globally, which is a considerable trigger of fibrosis, cirrhosis, and … ents. Furthermore, the most enriched genera in HCV-related cirrhosis patients were Bifidobacterium, Porphyromonas, and Bacteroides. MMP9 exhibited the highest diagnostic performance of the five measured biomarkers, discriminating against HCC vs. HCV-related cirrhosis with specificity and sensitivity of 100% and 90%, respectively, at a cut-off value > 166.8. Add …

Comparative evaluation of local drug delivery using tetracycline fibers and antimicrobial photodynamic therapy in Stage II Grade B periodontitis: A clinico-microbiological study.

BACKGROUND: Periodontal therapy aims to eliminate the periopathogens involved in disease progression to restore the lost form and function o … level, and probing pocket depth. Subgingival plaque samples were obtained for microbial analysis of Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Prevotella intermedia at the following periods. RESULTS: There was significant improvement in clinical and microbiological parameters in both the groups at 3 months and 6 months postoperatively …

GWAS of Serum Antibodies to Microbial Biomarker Species of Periodontitis.

Serum antibody levels against microbial biomarkers of periodontitis, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis, are associated especially with the presence of these species in the oral cavity. We investigated the genetic basis of host antibody responses against these species through a genome-wide association study (GWAS) to identify the genetic determinants of this immune reactivity. Serum immunoglobulin A (IgA) and immunoglobulin G (IgG) antibody levels against A. actinomycetemcomitans and P. gingivalis were determined using multiserotype enzyme-linked immunosorbent a…

Microbiome impacts quality of life in patients with endometrial cancer and benign gynecological conditions.

PURPOSE: Patients with endometrial cancer (EC), a prevalent gynecologic cancer in the United States, and benign gynecological conditions suc … cores were higher in the benign group. Vaginal species richness was higher in the EC group. Vaginal Porphyromonas negatively correlated to sexual interest in all patients, while Dialister B positively correlated to sexual interest in the benign group. Patients with EC and worse vaginal atrophy had increased vaginal L. iners, despite adjustment for menopausal st …

Hsa_circ_0007349 upregulates glutamate-ammonia ligase to modulate gingival fibroblast wound healing.

OBJECTIVES: Human gingival fibroblasts (HGFs) are important cells that maintain the structure and integrity of periodontal tissues. Circular … significantly increased in gingival tissues of patients with periodontitis and in HGFs treated with Porphyromonas gingivalis lipopolysaccharide. hsa_circ_0007349 regulates miR-127-5p to affect cell proliferation, migration, and wound healing, and affects extracellular matrix metabolism and periodontal tissue damage via GLUL. CONCLUSION: Hsa_circ_0007349 acts a …

Chemical characterization and antimicrobial activities of Citrus aurantifolia peel oils and Ocimum sanctum ethanolic extract.

Oral diseases affect more than 3.5 billion people globally, representing a major public health burden, particularly in low- and middle-incom … mum sanctum L. (OSE), against five oral pathogens: Lactobacillus acidophilus, Streptococcus mutans, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Candida albicans. Chemical constituents were analyzed using gas chromatography-mass spectrometry (GC-MS) for the peel oils and high-performance liquid chromatography (HPLC) for the extract. GC-M …

ACSL6-Mediated Cell Ferroptosis by Inhibiting AMPK Pathway in Periodontitis Stem Cells.

BACKGROUND: Periodontitis is an immunoinflammatory disease. Ferroptosis is a type of inflammation-associated cell death. The article aims to … , a ferroptosis-related gene, exhibited high expression levels in periodontitis tissues (p < 0.05). Porphyromonas gingivalis lipopolysaccharide (P. gingivalis-LPS) upregulated ACSL6 (p < 0.05), downregulated ferroptosis-related genes (glutathione peroxidase 4 (p < 0.001) and cystine/glutamate transporter (Solute Carrier Family 7 Member 11) (p < 0.01)) and phosp …

3D Peri-Implant Epi-Mucosa-on-a-Chip Reveals Alterations in Epithelial Barrier Function Mediated by Host-Bacteria-Biomaterial Interactions.

Peri-implantitis is characterized by disruption of the epithelial barrier at the implant-mucosa interface, driven by complex interactions be … lammatory mechanical stress, independently impaired epithelial cohesion, whereas the combination of Porphyromonas gingivalis (P. gingivalis) and implant-derived titanium microparticles (i-TiPs) synergistically exacerbated barrier breakdown. i-TiPs also altered matrix architecture and stiffness, further compromising epithelial integrity and potentiating bacteria …

Множественный склероз, излучаемый повторно, ассоциируется с дисбиотическим оральным микробиомом.

ЦЕЛЬ: Множественный склероз (MS) — хроническое аутоиммунное расстройство, характеризующееся воспалением, обезвоживанием и неврологическим расстройством. В то время как роль кишечной микробиоты в MS подробно изучается, связь между пероральной микробиотой и MS по-прежнему недооценивается, особенно в североамериканских группах. Цель этого исследования заключалась в изучении микробиота (бакериального) состава, а также функциональных путей и иммунных профилей пероральной полости у 60 пациентов с релапсирующим MS (RRMS), стратифицированным по состоянию лечения, по сравнению с 44 здоровыми средствами

Совпадение в аннотации оригинала
OBJECTIVE: Multiple sclerosis (MS) is a chronic autoimmune disorder characterized by inflammation, demyelination, and neurological impairmen … a higher Bacteroidota to Firmicutes ratio. Key taxa such as Campylobacter, Lachnoanaerobaculum, and Porphyromonas were enriched in RRMS. Functional profiling revealed 49 differentially abundant pathways, including the enrichment of lipopolysaccharide biosynthesis in MS. Elevated levels of IFN-γ, IL-6, and other cytokines correlated with the altered microbiome. …

Effect of toxins from different periodontitis-associated bacteria on human platelet function.

BACKGROUND: Periodontitis is caused by a dysbiosis of oral bacteria resulting in alveolar bone destruction and teeth loss. The role of plate … ion of the C14-Tri-LAN-Gly peptide from Fusobacterium nucleatum (C14), and lipopolysaccharides from Porphyromonas gingivalis (LPS). METHODS: Light transmission aggregometry was performed after the addition of toxins to platelet-rich plasma in different doses. Flow cytometry was used to identify inhibitory effects of toxins by measuring phosphorylation of the v …

Integrative microbiome and metabolome profiles reveal the impacts of periodontitis via oral-gut axis in first-trimester pregnant women.

BACKGROUND: Periodontitis results from host-microbe dysbiosis and the resultant dysregulated immunoinflammatory response. Importantly, it cl … Lachnoclostridium could discriminate between Perio and Non-Perio groups as the ratio of subgingival Porphyromonas to Rothia did. Furthermore, there were differentially abundant fecal metabolic features pivotally enriched in periodontitis subjects like L-urobilin and kynurenic acid. We revealed a periodontitis-oriented integrative network cluster, which was cent …

Antibacterial and cytotoxicity properties of a polyherbal mouthwash containing Achyranthes aspera and Trachyspermum ammi against selected periodontal pathogens.

BACKGROUND: Chlorhexidine (CHX) is considered as a gold standard for its antibacterial efficacy and substantivity in chemical plaque control … ects of novel polyherbal mouthwash containing A. aspera and T. ammi with 0.2% CHX mouthwash against Porphyromonas gingivalis, Tannerella forsythia, and Aggregatibacter actinomycetemcomitans. METHODS: Ethanolic extracts of A. aspera and T. ammi were prepared by the Soxhlet apparatus method and were subjected to preliminary phytochemical screening. The individua …

HDAC6-Mediated FoxO1 Acetylation And Phosphorylation Control Periodontal Inflammatory Responses.

Post-translational modifications (PTMs) are critical regulators of protein function and cellular signaling. While histone deacetylation by histone deacetylases (HDACs) is well established, the role of specific HDACs in modulating non-histone protein PTMs, particularly in an infectious context, is poorly understood. Here, we reveal a pivotal role for HDAC6 in orchestrating periodontal inflammation through its dual regulatory effects on FoxO1 acetylation and phosphorylation. Using Porphyromonas gingivalis , a key periodontal pathogen, as a model pathogen, we observed that infection induces HDAC6…

Oral microbiota in preschoolers with rampant caries: a matched case-control study.

Rampant caries is identified by rapid onset, severe decay affecting multiple surfaces, and early pulp infection. This case-control study was … , positively correlated with the frequency of candy consumption. Furthermore, Streptococcus mutans, Porphyromonas gingivalis, and Bacteroides acidifaciens were identified as potential oral microbiome markers for differentiating preschoolers with rampant caries from their caries-free peers. B. acidifaciens, typically found in the gut, has been rarely reported in …

Curcumin is effective in managing oral inflammation: An in vitro study.

BACKGROUND: Oral inflammation is among the most prevalent oral pathologies with systemic health implications, necessitating safe and effecti … ormed along with antimicrobial efficacy tests targeting Candida albicans, Streptococcus mutans, and Porphyromonas gingivalis. RESULTS: LPS significantly reduced proliferation and wound healing capacities of HOKs, OKF6, and HPdLF, but not DOKs. Treatment with curcumin-based oral gel mitigated inflammatory responses in HOKs and HPdLF by enhancing proliferation, …

Increased IgA-mediated responses to the gut paracellular pathway and blood-brain barrier proteins predict delirium due to hip fracture in older adults.

INTRODUCTION: Delirium is accompanied by immune response system activation, which may, in theory, cause a breakdown of the gut barrier and b … bacterial cytolethal distending toxin (CDT), LPS-binding protein (LBP), lipopolysaccharides (LPS), Porphyromonas gingivalis, and Helicobacter pylori. RESULTS: Results from univariate analyses showed that delirium is linked to increased IgA responses to all the self-epitopes and antigens listed above, except for LPS. Part of the variance (between 45-48.3%) in …

Alterations in subgingival microbiome and advanced glycation end-products levels in periodontitis with and without type 1 diabetes mellitus: a cross-sectional study.

BACKGROUND: Existing studies predominantly focused on the relationship between periodontitis and type 2 diabetes mellitus (T2DM), with limit … roup: Acinetobacter, Leptotrichia, Raoultibacter, and Veillonella in the Control group; Tannerella, Porphyromonas, Filifactor, and Treponema in the P group; and Lactobacillales in T1DM individuals. Prevotella and Selenomonas were notably influential in the DP group. PICRUSt2 analysis showed pathways alterations were concentrated in cell motility, translation, c …