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LATS1/2-CD38 Metabolic Rewiring Links Senescence to Intraplaque Thrombosis.

BACKGROUND: Atherothrombosis, which underlies most acute coronary syndromes and is driven by intraplaque thrombosis, preferentially occurs i … s of Lats1/2 using inducible EC-specific knockout mice in a partial carotid ligation model. Spatial multiomics of human and mouse plaques was performed using imaging mass cytometry, COMET sequential immunofluorescence, and spatial metabolomics. RESULTS: Tamoxifen-induced deletion of both Lats1 and Lats2 (homozygous) in ECs caused fatal edema and increased v …

Ketogenic diet improves cognitive impairment in rats with temporal lobe epilepsy by activating the Nrf2/HO-1/GPX4 signaling axis to inhibit ferroptosis.

A ketogenic diet (KD) has demonstrated significant therapeutic efficacy in drug-resistant epilepsy. The molecular mechanisms through which K … the Morris water maze and novel object recognition tests. With respect to the underlying mechanism, multiomics analysis revealed that KDs alter circulating metabolite profiles. Notably, we revealed that deoxycholyl-L-dopa may be a key metabolite for targeting Keap1, xCT and HO-1. Western blot and qPCR results revealed that KDs activated the Nrf2/HO-1/GPX4 si …

Sodium Danshensu Attenuates Renal Ischemia-Reperfusion Injury by Suppressing STAT3-Driven Cytokine Signaling and Apoptosis.

OBJECTIVE: To evaluate the renoprotective effects and underlying molecular mechanisms of sodium danshensu (SDSS) in renal ischemia-reperfusi … confirming the involvement of the STAT3 pathway. RESULTS: The results of network pharmacology and multiomics analysis identified STAT3, P-STAT3, RELA proto-oncogene (RELA), C-C motif chemokine ligand 2 (CCL2), tumor necrosis factor (TNF), BAX, and CASP3 as therapeutic targets involved in inflammation, apoptosis and immune regulation during RIR progression. …

Current Clinical Perspectives of Biomarkers in Respiratory Diseases: A Narrative Review.

Respiratory medicine is transitioning from symptom-driven, standardized care to a more precise, patient-specific approach guided by molecular profiling. This evolution is being enabled by advances in liquid biopsy, multiomics, and artificial intelligence (AI) analytics. Fractional exhaled nitric oxide (FeNO) and blood eosinophils, the two commonly used markers in asthma, are now being joined by more precise airway markers such as galectin-10, which could aid clinicians in making more informed decisions for biological treatments. In chronic obstructive pulmonary disease (COPD) similar progress …

GPR84 and Neuroinflammation: A Receptor Worth Targeting, or A Target Worth Reconsidering?

GPR84 is a medium-chain fatty acid (MCFA)-sensing G-protein-coupled receptor conditionally expressed on innate immune cells - neutrophils, m … tional genetic models, CNS-penetrant pharmacological tools, and integration of spatial and temporal multiomics approaches before the receptor's therapeutic potential can be meaningfully evaluated.

Dysbiosis-Associated Lysophosphatidic Acid Primes Immune Checkpoint Inhibitor-Induced Intestinal Barrier Dysfunction via the Lysophosphatidic Acid Receptor 2-Myosin Light Chain Kinase Axis.

BACKGROUND & AIMS: Immune checkpoint inhibitor therapy is frequently limited by severe gastrointestinal toxicity. Although the immune mechanisms driving late-stage, overt mucosal inflammation are increasingly recognized, the early microbiota-metabolic events that prime epithelial barrier dysfunction remain poorly defined. METHODS: We employed an integrated multiomics approach in a murine model of early immune checkpoint inhibitor-induced injury, with validation in a clinical cohort of patients with immune checkpoint inhibitor colitis. Mechanisms were investigated using in vitro receptor scree…

Mechanistic Landscape and Farm to Fork Control Strategies for Streptococcus suis: An Emerging Foodborne Threat.

Streptococcus suis (S. suis) is an emerging zoonotic agent that now rivals classical food-borne pathogens in its global clinical burden of m … trol of biofilm maturation, and metabolic reprogramming that fuels neutrophil evasion. We integrate multiomics signatures with structural data to map conserved targets such as IdeSui and capsular polysaccharide that underpin next-generation conjugate and nanoparticle vaccines. Diagnostic advances spanning CRISPR-based assays and high-resolution imaging are a …

Регулирование на основе Virobiome микробиота-гут-мозговой оси сигнализирует и нейроиммунный гомеостаз.

Ось человеческого кишечника все чаще признается как сложная двунаправленная коммуникационная система, объединяющая микробные, нейронные, эндокринные и иммунные сети, формирующие неврологическое здоровье. Хотя бактериальный вклад в этот диалог получил широкую окраску, вирусный компонент кишечной экосистемы, виром, стал важным компонентом, связанным с физиологическим регулированием принимающей стороны. В этом обзоре обобщены данные, свидетельствующие о том, что бактериофаги и эукариотические вирусы могут способствовать микробному и иммунальному гомеостазу и оказывать влияние на сигнализацию вдол

Совпадение в аннотации оригинала
The human gut-brain axis (GBA) is increasingly recognized as a complex bidirectional communication system integrating microbial, neural, end … e being explored as potential approaches to modulate virome-microbiome interactions. Integration of multiomics platforms with artificial intelligence-driven modeling will be critical for clarifying the temporal and mechanistic relationships between virome dynamics and neurological function. Collectively, these insights highlight the gut virobiome as a potent …

Cross-species engraftment biases and metabolic divergence in gnotobiotic mice humanized with ulcerative colitis microbiota.

Ulcerative colitis (UC) is a chronic inflammatory disease of the human colon. Dysbiotic gut microbiota play a central role in its pathogenesis, and alterations in microbial composition and function are closely linked to disease activity. Humanized gnotobiotic mice are increasingly used to study how dysbiotic, human-derived microbial communities shape intestinal inflammation. However, the fidelity of microbiota engraftment and its impact on host physiology and metabolism remain incompletely understood. In this study, we performed a multiomics analysis following fecal microbiota transfer (FMT) f…

Pharmacological activation of STAT1-GSDME pyroptotic circuitry reinforces epigenetic immunotherapy for hepatocellular carcinoma.

BACKGROUND: Genomic screening uncovered interferon-gamma (IFNγ) pathway defects in tumours refractory to immune checkpoint blockade (ICB). H … inhibition in 4 ICB-resistant orthotopic and spontaneous models using immune profiling, single-cell multiomics and chromatin immunoprecipitation-sequencing and verified by genetic modulations and co-culture systems. RESULTS: HCC patients showing higher HDAC1/2/3 expressions exhibited deficient IFNγ signalling and poorer survival on ICB therapy. Transient tr …

Shared molecular profiles of post-laser vision correction ectasia and keratoconus with key differences in CADPS, CPT1B, CIITA, and TBC1D4.

INTRODUCTION: Post-laser vision correction (post-LVC) ectasia is a serious complication that is observed in 0.033%-0.66% of corneal refractive surgeries. Similar to keratoconus (KTCN), post-LVC ectasia is classified under the category of "ectatic diseases." We hypothesize that although the mechanistic aspects of post-LVC ectasia and KTCN are distinct, there are notable similarities in the epithelial responses, including shared molecular features. METHODS: A total of 11 post-LVC ectasia, 8 mild myopia (controls), and 28 KTCN patients were included in a retrospective multiomics case-control stu…

24-Nor-ursodeoxycholic acid improves intestinal inflammation by targeting TH17 pathogenicity and transdifferentiation.

BACKGROUND: 24-Nor-ursodeoxycholic acid (NorUDCA) is a novel therapeutic bile acid for treating immune-mediated cholestatic liver diseases, … timulation model combined with interleukin-17A-fate-mapping. Mechanistic studies used molecular and multiomics approaches, flow cytometry and metabolic assays with pathogenic (p) TH17. Pathogenicity of pTH17 exposed to NorUDCA in vitro was evaluated following adoptive transfer in intestinal tissues or the central nervous system (CNS). Key findings were valid …

Kun-Qi birth cohort (KQBC) study for the incidence, aetiology and risk factors of chronic kidney disease in Chinese children: a cohort profile.

PURPOSE: The Kun-Qi birth cohort (KQBC) was established to investigate the incidence, aetiology and risk factors of chronic kidney disease ( … , aetiology and risk factors of early onset CKD. In-depth developmental phenotypes, such as urinary multiomics and ultrasound imaging, should be studied for longitudinal paediatric kidney health. We aim to develop multimodal risk prediction models by integrating genomics and artificial intelligence algorithms. The KQBC also enables prospective cohort studies …

Spatiotemporal disarray of inflammatory microenvironment following spinal cord injury.

Spinal cord injury is a severe neurological condition with far-reaching consequences for both individuals and society. Its progression invol … flammation is essential for designing targeted interventions. Future directions include integrating multiomics datasets, identifying predictive biomarkers for patient stratification, and developing adaptive treatment protocols that fine-tune rather than suppress immune responses to promote neural repair and functional recovery.

Targeting Hepatic Stellate Cell PD-L1 Alters Liver Inflammation and Fibrosis in CCl4 Liver Injury Mouse Model.

BACKGROUND & AIMS: Programmed death-ligand 1 (PD-L1) on hepatic stellate cells (HSCs) is required for HSC activation and suppressing T and B … ion markers; liver inflammation was studied by multiplex immunofluorescence and cytokine profiling. Multiomics was leveraged to determine how targeting PD-L1 altered HSC producing collagens and cytokines/chemokines. RESULTS: Collagen deposition was reduced in CCl4-injured PD-L1HSCKO livers compared with CCl4-injured PD-L1HSCWT livers; myofibroblast density …

An integrated multi-omics biomarker approach using molecular profiling and microRNAs for evaluation of pancreatic cyst fluid.

BACKGROUND: Classification and risk stratification of pancreatic cysts are challenging because of limited radiographic and cytomorphologic f … al utility of miRNAs as biomarkers in PCF analysis. CONCLUSIONS: These findings demonstrate that a multiomics biomarker approach with elevated PCF miRNAs with mutant KRAS, mutant GNAS, and serum CA19-9 may help in better detecting high-risk cysts for early clinical intervention.

Transcriptomic and proteomic signatures of host NK cells delineate distinct immune states across tuberculosis infection statuses.

INTRODUCTION: Although natural killer (NK) cells play crucial roles in the immune response to Mycobacterium tuberculosis (M.tb) infection, systematic investigations delineating the immune characteristics of NK cells across the tuberculosis (TB) disease spectrum are scarce. METHODS: This multiomics study employed transcriptomic, proteomic, and RT-qPCR analyses to characterize and validate CD56+ NK cells from 165 participants stratified by TB infection status (active TB (ATB), latent TB infection (LTBI), and healthy control (HC)). Peripheral blood samples from an independent cohort of 85 partic…

Translating genetic findings to epigenetics: identifying the mechanisms associated with aging after high-radiation exposure on earth and in space.

PURPOSE: Exposure to radiation is a health concern within and beyond the Earth's atmosphere for aircrew and astronauts in their respective austere environments. The biological effects of radiation exposure from a multiomics standpoint are relatively unexplored and stand to shed light on tailored monitoring and treatment for those in these career fields. To establish a reference variable for genetic damage, biological age seems to be closely associated with the effect of radiation. Following a genetic-based study, this study explores the epigenetic landscape of radiation exposure along with its…

Checkpoint Kinase 1 Stimulates Endogenous Cardiomyocyte Renewal and Cardiac Repair by Binding to Pyruvate Kinase Isoform M2 C-Domain and Activating Cardiac Metabolic Reprogramming in a Porcine Model of Myocardial Ischemia/Reperfusion Injury.

BACKGROUND: The regenerative capacity of the adult mammalian hearts is limited. Numerous studies have explored mechanisms of adult cardiomyo … deling, and reduced the infarct area at 28 days after ischemia/reperfusion injury. Mechanistically, multiomics sequencing analysis demonstrated enrichment of glycolysis and mTOR (mammalian target of rapamycin) pathways after rhCHK1 treatment. Co-Immunoprecipitation (Co-IP) experiments and protein docking prediction showed that CHK1 (checkpoint kinase 1) dire …

The effect of lipid metabolism disorder on patients with hyperuricemia using Multi-Omics analysis.

A multiomics study was conducted to investigate how lipid metabolism disorders affect the immune system in Xinjiang patients with hyperuricemia. The serum of 60 healthy individuals and 60 patients with hyperuricemia was collected. This study used LC-MS and HPLC to analyze differential lipid metabolites and enrichment pathways. It measured levels of immune factors tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), carnitine palmitoyltransferase-1 (CPT1), transforming growth factor-β1 (TGF-β1), glucose (Glu), lactic acid (LD), interleukin 10 (IL-10), and selenoprotein 1 (SEP1) using ELISA, a…